Molecular detection of <i>Helicobacter pylori</i> and clarithromycin resistance in gastric biopsies: a prospective evaluation of RIDA®GENE <i>Helicobacter pylori</i> assay

Poel, Bea Van den; Gils, Sarah; Micalessi, Isabel; Carton, Saskia; Christiaens, Paul; Cuyle, Pieter-Jan; Moons, Veerle; Olmen, Gust Van; Smismans, Annick; Bourgain, Claire; Bossuyt, Peter; Frans, Johan

doi:10.1080/17843286.2019.1685741

Cited by 16 publications

(13 citation statements)

References 35 publications

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“…and the first three are the most frequent mutations. [13][14][15][16] The fluoroquinolone resistance of H. pylori depends on the point mutations in the quinolone resistance-determining region of the gyrA gene at the positions encoding these amino acids, and the most frequent mutations are found at codons 87 and 91 of the gyrA gene. 17 There are regional differences in H. pylori resistance-related mutation sites 18-20 and the regional differences should be focused upon to avoid false negatives.…”

Section: Discussionmentioning

confidence: 99%

Correlation Analysis Among Genotype Resistance, Phenotype Resistance and Eradication Effect of Helicobacter pylori

Cui¹,

Song²,

Suo³

et al. 2021

IDR

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Background: It has not been fully confirmed whether the detection of Helicobacter pylori resistance gene mutation can replace antibiotic drug sensitivity test to guide the clinical individualized treatment. Therefore, we have studied this aspect and discussed the application value of antibiotic sensitivity gene test. Materials and Methods: The biopsy specimen of gastric mucosa from the patients examined by endoscopy and positive for rapid urease test were collected continuously for histopathological analysis, H. pylori culture, antibiotic drug sensitivity test (E-test drug sensitivity test), and antibiotic sensitivity gene test (high-throughput nucleotide sequencing). The participants received triple plus bismuth solution eradication treatment (esomeprazole 20 mg, amoxicillin 1000 mg, clarithromycin 500 mg, and bismuth potassium citrate 220 mg, twice daily for 14 days) for follow-up, and the eradication effect was determined. Results: The 551/602 subjects, who met the inclusion criteria, were subjected to culture for H. pylori and antibiotic drug sensitivity determination; among them 506 were cultured successfully. The results showed that the resistance rates of H. pylori were 38.9% for clarithromycin and 31.0% for levofloxacin. In 489 H. pylori strains, the mutations were detected in clarithromycin and levofloxacin resistance genes, indicating the genotype resistance. The resistance genes of clarithromycin and levofloxacin were consistent with phenotype resistance with respect to sensitivity (81.2% and 69.7% for clarithromycin and levofloxacin, respectively) and specificity (88.9% and 93.7% for clarithromycin and levofloxacin, respectively). The eradication rate of H. pylori in the clarithromycin-resistant group was significantly lower than that in the sensitive group (ITT: 52.1% vs 85.0%, P < 0.001). Conclusion:A correlation was established between the resistance genes of clarithromycin and levofloxacin and their phenotypic resistance and clinical efficacy. The detection of H. pylori resistance genes has a good clinical application prospect.

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Section: Discussionmentioning

confidence: 99%

Correlation Analysis Among Genotype Resistance, Phenotype Resistance and Eradication Effect of Helicobacter pylori

Cui¹,

Song²,

Suo³

et al. 2021

IDR

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“…and Seeplex® H. pylori-ClaR ACE Detection (Seegene, South Korea) are real-time PCR kits for simultaneous detection of H. pylori and its resistance to clarithromycin caused by point mutations in the 23S rRNA gene. [47][48][49][50] It has been reported that the molecular susceptibility results using the GenoType HelicoDR kit (Hain Life Sciences, Germany) on gastric biopsies correlates well with the culturedependent AST results. 51,52 In addition, PyloriAR assay (American Molecular Laboratories), a Next-Generation Sequencing service using formalin-fixed paraffin-embedded gastric biopsies, is now available to test six genes (23S rRNA, gyrA, rdxA, pbp1, 16S rRNA, and rpoB) in the H. pylori genome for variants that are associated with H. pylori antibiotic resistance.…”

Section: Predic Tion Of Antimicrob Ial Suscep Tib Ilit Y Us Ing Molec...mentioning

confidence: 97%

“…pylori ‐ClaR ACE Detection (Seegene, South Korea) are real‐time PCR kits for simultaneous detection of H . pylori and its resistance to clarithromycin caused by point mutations in the 23S rRNA gene 47–50 . It has been reported that the molecular susceptibility results using the GenoType HelicoDR kit (Hain Life Sciences, Germany) on gastric biopsies correlates well with the culture‐dependent AST results 51,52 .…”

Section: Prediction Of Antimicrobial Susceptibility Using Molecular M...mentioning

confidence: 99%

Need for standardization and harmonization of Helicobacter pylori antimicrobial susceptibility testing

Shen

Song

et al. 2022

Helicobacter

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Background and aims As with other infectious diseases, Helicobacter pylori eradication regimens should be guided by susceptibility testing to achieve excellent success rate, especially in the era of high antibiotic resistance. However, susceptibility testing for H. pylori is rarely performed, which can be partly ascribed to the current lack of standardization of testing methods and the lack of unified consensus on the antibiotic resistance breakpoints. The aim of this review was to call for an international consensus on standardization and harmonization of H. pylori susceptibility testing. Methods We summarize and compare the advantages and disadvantages of four different phenotypic antimicrobial susceptibility testing (AST) methods (agar dilution, E‐test, disk diffusion, and broth microdilution) and the molecular susceptibility testing method for H. pylori. Results The standard phenotypic testing methods and the molecular testing methods have their own advantages and disadvantages. Compared to the standard phenotypic methods, the molecular testing method does not require successful H. pylori culture, and therefore, is much more rapid and convenient for clinical use. However, the currently available molecular testing method is only suitable for detecting clarithromycin and quinolone susceptibility profiles in H. pylori. Although the standard AST is time‐consuming, it is currently the only way to test the susceptibility of H. pylori to all the commonly used antibiotics. Conclusion To make H. pylori susceptibility testing become a clinical routine, an international consensus on standardization and harmonization of H. pylori AST is needed. Future efforts are needed for optimizing broth culture of H. pylori, and developing commercial AST plates for achieving high throughput and automated susceptibility testing for H. pylori.

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“…Finally, the presence of other non-H. pylori microorganisms carrying resistance genes or heteroresistance may lead to errors in the detection results. 145,146 With the development of a new generation of high-throughput molecular detection technology, next-generation sequencing (NGS) technology will gradually reveal its value. NGS enables the detection of H. pylori infection with high sensitivity and specificity and effectively predicts antibiotic resistance.…”

Section: Molecular Detectionmentioning

confidence: 99%

Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice

Lin

Shao

Yan

et al. 2023

Clinical Laboratory Analysis

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Helicobacter pylori (H. pylori) is a gram-negative microaerobic bacterium that is a common pathogen widely distributed around the world, especially in developing countries. 1 According to statistics from recent years, the H. pylori infection rate in developing countries exceeds 50%. 2 In West Asia, Africa, and South America, the H. pylori infection rate has been reported to be as high as 66.6%, 70.1%, and 69.4%, respectively. 3 H. pylori infection causes persistent damage to human gastric mucosa, leading to a variety of digestive

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Molecular detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies: a prospective evaluation of RIDA®GENE Helicobacter pylori assay

Cited by 16 publications

References 35 publications

Correlation Analysis Among Genotype Resistance, Phenotype Resistance and Eradication Effect of Helicobacter pylori

Correlation Analysis Among Genotype Resistance, Phenotype Resistance and Eradication Effect of Helicobacter pylori

Need for standardization and harmonization of Helicobacter pylori antimicrobial susceptibility testing

Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice

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