Molecular detection of <i>Bacteroides forsythus</i> in human periodontitis

Lotufo, Roberto Fraga Moreira; Flynn, Jane; Chen, C.; Slots, Jørgen

doi:10.1111/j.1399-302x.1994.tb00052.x

Cited by 52 publications

(46 citation statements)

References 32 publications

(52 reference statements)

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“…For example, 14 whole-genome DNA probes were validated by hybridization with 249 strains representing 51 species (90), while commercially available oligonucleotide probes were validated against panels containing fewer species (195,253,255,282). A whole-genome probe to B. forsythus showed no detectable reactivity with 75 strains representing 14 oral species (172). This limited evaluation means that some crossreactions with untested plaque species probably exist, and could contribute to the large number of false-positive results found when DNA probes are compared to culture results obtained on the same plaque samples (15,135,142,158,172,191,195,196,221,235,251,255,296,297,315,345; K. Backman, Letter, J. Periodontol.…”

Section: Dna Probesmentioning

confidence: 99%

“…The sensitivity ranged from 0.17 for A. actinomycetemcomitans to 0.86 for B. forsythus and Streptococcus sanguis, and the specificity ranged from 0.17 for P. intermedia to 1.0 for C. rectus. These ranges indicate the typical finding with DNA probes, namely, that they do not correlate well with culture or serological data, usually being positive when the culture or serological findings are negative, i.e., many false positives (142,158,172,179,191,195,221,255,345). The panel of 18 probes developed by Papapanou showed that only B. forsythus, P. gingivalis, T. denticola, and C. rectus (Wolinella recta) were associated with periodontal disease in 148 Chinese subjects who had never received any periodontal treatment (220).…”

Section: Specific Plaque Hypothesismentioning

confidence: 99%

“…A 5-to 10-min chairside test, called the BANA test (167a), can be used to diagnose an anaerobic infection involving P. gingivalis, B. forsythus, and T. denticola (151,156). While the BANA test does not indicate which of the three species is present, this may not be necessary since these species coexist in the same plaques (97,172,265,279) and all are anaerobes. A positive BANA assay was one of the significant predictors of whether a clinician would recommend a tooth for periodontal surgery or for extraction (165).…”

Section: Enzyme Assaysmentioning

confidence: 99%

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Periodontal Disease as a Specific, albeit Chronic, Infection: Diagnosis and Treatment

Loesche

Grossman²

2001

Clin Microbiol Rev

398

336

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Periodontal disease is perhaps the most common chronic infection in adults. Evidence has been accumulating for the past 30 years which indicates that almost all forms of periodontal disease are chronic but specific bacterial infections due to the overgrowth in the dental plaque of a finite number of mostly anaerobic species such as Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola. The success of traditional debridement procedures and/or antimicrobial agents in improving periodontal health can be associated with the reduction in levels of these anaerobes in the dental plaque. These findings suggest that patients and clinicians have a choice in the treatment of this overgrowth, either a debridement and surgery approach or a debridement and antimicrobial treatment approach. However, the antimicrobial approach, while supported by a wealth of scientific evidence, goes contrary to centuries of dental teaching that states that periodontal disease results from a “dirty mouth.” If periodontal disease is demonstrated to be a risk factor for cardiovascular disease and stroke, it will be a modifiable risk factor since periodontal disease can be prevented and treated. Since the antimicrobial approach may be as effective as a surgical approach in the restoration and maintenance of a periodontally healthy dentition, this would give a cardiac or stroke patient and his or her physician a choice in the implementation of treatment seeking to improve the patient's periodontal condition so as to reduce and/or delay future cardiovascular events

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Section: Dna Probesmentioning

confidence: 99%

Section: Specific Plaque Hypothesismentioning

confidence: 99%

Section: Enzyme Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Periodontal Disease as a Specific, albeit Chronic, Infection: Diagnosis and Treatment

Loesche

Grossman²

2001

Clin Microbiol Rev

398

336

View full text Add to dashboard Cite

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“…This organism is often isolated from active periodontitis sites (5), and it is also reported that the presence of B. forsythus is a risk factor for periodontal disease (7,8,29). B. forsythus is frequently isolated with Porphyromonas gingivalis, which is one of the important periodontopathic bacteria (4,16), and these two bacteria are known to have a synergistic effect in animal models (27,34).…”

mentioning

confidence: 99%

Humoral Immune Responses to S-Layer-Like Proteins of Bacteroides forsythus

Yoneda

Hirofuji

Motooka

et al. 2003

Clin Vaccine Immunol

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Bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an S-layer outside the outer membrane. The S-layer-like antigens were recently isolated from B. forsythus, and they were found to be 270-and 230-kDa proteins in the envelope fraction. In this study, these proteins were confirmed to be specific to B. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adult and early-onset periodontitis in Western immmunoblot analysis. We compared the immunoglobulin G (IgG) responses against the purified S-layer-like antigen by enzyme-linked immunosorbent assay. IgG responses against this antigen were low in healthy control subjects, but they were significantly higher in subjects with adult and early-onset periodontitis. Together with the fact that the IgG responses against the crude extract of B. forsythus did not rise significantly in patients with periodontitis, S-layer-like proteins are considered to be specific antigens of B. forsythus and may play an important role in the progression of periodontitis.

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“…Several methods, including random amplified polymorphic DNA (RAPD), arbitrary primed PCR (AR-PCR), restriction fragment length polymorphism (RFLP), PCR-RFLP and DNA sequencing, have recently been used for the authentication of biological materials (Lotufo et al 1994;Lakra et al 2007;Taylor and Ford, 2007). Although they have been proven to be efficient in taxonomic identification and in distinguishing genuine crude drugs from their substitutes or adulterants in previous reports, the application of these methods is limited by the high cost of the fine quality template DNA that is required in these experiments.…”

Section: Discussionmentioning

confidence: 99%