Molecular Detection of Human Papillomavirus and Viral DNA Load after Radiotherapy for Cervical Carcinomas

Kahla, Saloua; Kochbati, L.; Sarraj, Sana; Daya, Imen Ben; Maâlej, M.; Oueslati, Ridha

doi:10.5301/tj.5000401

Cited by 8 publications

(11 citation statements)

References 25 publications

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“…In the present study, the HPV test was based on qRT-PCR, which con rmed that 85.3% of patients were HPV-positive(266/312). Among the pre-operative HPV-positive patients, 85.3% of them were HPV-16/18 positive, which was concordance with other studies(70%-90%) [13,15,[19][20][21].…”

Section: Discussionsupporting

confidence: 92%

“…The infection of hr-HPV was crucial for carcinogenesis in cervical cancer [4,10,14,15]. Though the association between HPV infection status and the prognosis of early-stage cervical had been studied through HPV tests, the conclusions remained controversial for decades [7,9,13,16,17]. HC2-based or PCR-based HPV tests were adopted by most studies, but HC2 could only do a typing test, while PCR could do both typing and quantifying tests [18].…”

Section: Discussionmentioning

confidence: 99%

“…In our study, post-operative persistence of high HPV-DNA level within 12 m was an independent risk factor for local recurrence(p = 0.011). Kahla et al (2016) also found that high initial HPV-16/18 viral load was associated with a poor DFS(p = 0.04), but not with a poor OS(p = 0.2) [13]. However, Mahantshetty et al(2018) found that both of HPV-16 viral load and HPV-18 viral load do not have impact on DFS(p = 0.65, p = 0.14) or OS(p = 0.93, p = 0.32) [12].…”

Section: Discussionmentioning

confidence: 99%

“…However, HPV viral load also had an impact on the prognosis of cervical cancer patients [7][8][9][10][11]. Though many previous studies try to clarify the in uence of HVP viral load, conclusions about the prognostic value of HPV viral load remained controversial for years [7,12,13].…”

Section: Introductionmentioning

confidence: 99%

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The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Zeng¹,

Zuo²,

Li³

et al. 2020

Preprint

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Background: High-risk HPV(hr-HPV) infection is important for the development of invasive cervical cancer. As a developing country with the largest population in the world, China has a great burden of cervical cancer. The cervical cancer screening strategies consisted of cytology and virology, which has been studied by HPV-typing test and HPV-DNA quantitative test since decades ago. The cervical cancer incidence rate has been declined due to the early treatment of precancerous lesions, but postoperative HPV infection is still an issue for cervical cancer patients. This study aims to investigate the association between HPV infection status and recurrence of early-stage cervical cancer through a novel PCR-based HPV test, which could do both HPV typing-test and DNA quantitative-test. Methods: Patients diagnosed with cervical cancer staged IB1- IIA2, who were treated by radical hysterectomy and lymphadenectomy at Cancer Hospital Chinese Academy of Medical Sciences(CAMS) between January 2014 and December 2016 were accrued. The clinicopathological factors, pre- and post-operative HPV infection status, and the prognosis were investigated. Cox regression was used to identify factors associated with LRFS, MFS and OS. Results: A total of 312 patients were enrolled in this study, who were treated by radical resection and accepted pre- and post-operative HPV tests, with a median follow-up time of 60 months(range 14~79 months). The 5-year LRFS rate, MFS rate, and OS rate were 97.8%, 98.4%, and 98.7%. The pre-operative HPV infection rate was 85.3%(266/312), 74 patients had a high level of HPV-DNA(>5x106 copy number/104 cells). Twenty-nine patients had a postoperative persistent high level of HPV-DNA(9.3%). Postoperative persistent high level of HPV-DNA within 12 months(p=0.013), postoperative persistence of HPV-16/18 within 24 months(p=0.004), and deep stromal invasion(>2/3)(p=0.007) were associated with a poor LRFS.Conclusion: Pre-operative HPV-16/18 infection and high level of HPV-DNA were not associated with local recurrence of cervical cancer. Most initial HPV-positive patients had HPV cleared within 24 months postoperatively. Postoperative HPV-16/18 persistence within 24 months, postoperative persistence of high HPV-DNA level within 24 months, and deep stromal invasion(>2/3) were independent risk factors for local recurrence of cervical cancer.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Zeng¹,

Zuo²,

Li³

et al. 2020

Preprint

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“…Therefore, the HR HPV RT PCR assay provides a potential tool to compare the efficacy of vaginitis treatment and to facilitate the diagnosis of HPV infections. However, the significance of HPV viral load in the prediction of the response to current therapeutic approaches of HPV infections, such as radiotherapy, has not reached a consensus, which warrants further investigations.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the performance in detection of HPV infections between the high‐risk HPV genotyping real time PCR and the PCR‐reverse dot blot assays

Zhang

Dai

Chen

et al. 2017

Journal of Medical Virology

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A new multiplex real-time PCR assay, the high-risk HPV genotyping real time PCR assay (HR HPV RT-PCR), has been developed to detect 15 high-risk HPV types with respective viral loads. In this report, a total of 684 cervical specimens from women diagnosed with vaginitis were assessed by the HR HPV RT-PCR and the PCR reaction and reverse dot blot (PCR-RDB) assays, using a PCR-sequencing method as a reference standard. A total coincidence of 97.7% between the HR HPV RT PCR and the PCR-RDB assays was determined with a Kappa value of 0.953. The HR HPV RT PCR assay had sensitivity, specificity, and concordance rates (accuracy) of 99.7%, 99.7%, and 99.7%, respectively, as confirmed by PCR-sequencing, while the PCR-RDB assay had respective rates of 98.8%, 97.1%, and 98.0%. The overall rate of HPV infection, determined by PCR-sequencing, in women diagnosed with vaginitis was 49.85%, including 36.26% of single infection and 13.6% of multiple infections. The most common infections among the 15 high-risk HPV types in women diagnosed with vaginitis were HPV-52, HPV-16, and HPV-58, with a total detection rate of 10.23%, 7.75%, and 5.85%, respectively. We conclude that the HR HPV RT PCR assay exhibits better clinical performance than the PCR-RDB assay, and is an ideal alternative method for HPV genotyping. In addition, the HR HPV RT PCR assay provides HPV DNA viral loads, and could serve as a quantitative marker in the diagnosis and treatment of single and multiple HPV infections. . 2 Meta-analyses have shown that there is a positive association between vaginitis and cervical HPV infection, and that the pooled prevalence of vaginitis can be as high as 32%. [3][4] In addition to single infection, cervical co-infections of multiple HPV genotypes is common. 5 J Med Virol. 2018;90:177-183.wileyonlinelibrary.com/journal/jmv

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Increased human papillomavirus viral load is correlated to higher severity of cervical disease and poorer clinical outcome: A systematic review

Fobian,

Mei,

Crezee

et al. 2024

Journal of Medical Virology

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Cervical cancer is the fourth most common cancer in women worldwide and is caused by persistent infection with high‐risk types of human papillomavirus (HPV). HPV viral load, the amount of HPV DNA in a sample, has been suggested to correlate with cervical disease severity, and with clinical outcome of cervical cancer. In this systematic review, we searched three databases (EMBASE, PubMed, Web of Science) to examine the current evidence on the association between HPV viral load in cervical samples and disease severity, as well as clinical outcome. After exclusion of articles not on HPV, cervical cancer, or containing clinical outcomes, 85 original studies involving 173 746 women were included. The vast majority (73/85 = 85.9%) reported that a higher viral load was correlated with higher disease severity or worse clinical outcome. Several studies reported either no correlation (3/85 = 3.5%), or the opposite correlation (9/85 = 10.6%); possible reasons being different categorization of HPV viral load levels, or the use of specific sampling methods. Despite variations in study design and populations, the above findings suggest that HPV viral load is correlated to clinical outcome, and may become an important biomarker for treatment selection and response monitoring for cervical cancer.

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Molecular Detection of Human Papillomavirus and Viral DNA Load after Radiotherapy for Cervical Carcinomas

Abstract: Our data provide prospective evidence that ionizing radiation can affect the HPV viral load and this might offer the best strategies for assessment of therapeutic efficacy.

Cited by 8 publications

References 25 publications

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

Comparison of the performance in detection of HPV infections between the high‐risk HPV genotyping real time PCR and the PCR‐reverse dot blot assays

Increased human papillomavirus viral load is correlated to higher severity of cervical disease and poorer clinical outcome: A systematic review

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Molecular Detection of Human Papillomavirus and Viral DNA Load after Radiotherapy for Cervical Carcinomas

Abstract: Our data provide prospective evidence that ionizing radiation can affect the HPV viral load and this might offer the best strategies for assessment of therapeutic efficacy.

Cited by 8 publications

References 25 publications

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2&nbsp;Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2&nbsp;Cervical Squamous Cell Carcinoma

Comparison of the performance in detection of HPV infections between the high‐risk HPV genotyping real time PCR and the PCR‐reverse dot blot assays

Increased human papillomavirus viral load is correlated to higher severity of cervical disease and poorer clinical outcome: A systematic review

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The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma

The Prognostic Value of High-risk Human Papillomavirus Infection Status in Surgically Treated Stage IB1-IIA2 Cervical Squamous Cell Carcinoma