Molecular detection of human papillomavirus in 594 uterine cervix samples from Moroccan women (147 biopsies and 447 swabs)

Amrani, M.; Lalaoui, K.; Mzibri, Mohammed El; Lazo, Pedro A.; Belabbas, Mohamed Alaoui

doi:10.1016/s1386-6532(02)00227-5

Cited by 20 publications

(21 citation statements)

References 23 publications

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“…HPV DNA was amplified by PCR using consensus primers MY09 and MY11, and HPV genotyping was realized by Southern blot hybridization using specific probes as described by Amrani et al (2).…”

Section: Hpv Detectionmentioning

confidence: 99%

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Status of p16<SUP>INK4a</SUP> and E-Cadherin Gene Promoter Methylation in Moroccan Patients With Cervical Carcinoma

Attaleb¹,

W²,

Khyatti³

et al. 2009

oncol res

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Aberrant methylation of tumor suppressor gene promoters has been extensively investigated in cervical cancer. Transcriptional silencing, as a main consequence of hypermethylation of CpG islands, is the predominant mechanism of p16 INK4a and E-cadherin gene inactivation in malignant epithelial tumors. This study was conducted to evaluate the promoter methylation status of p16 INK4a and E-cadherin genes in 22 specimens of cervical carcinomas, four cervical cancer cell lines (HeLa, SiHa, Caski, C33A), and 20 human papillomavirus negative specimens, obtained from normal cervical swabs, using the methylation-specific PCR approach. Hypermethylation of the 5′ CpG island of the p16 INK4a and E-cadherin genes were found in 13 (59.1%) and 10 (45.5%) of 22 cervical cancer samples, respectively. Furthermore, our findings did not show any correlation between promoter methylation of p16 INK4a and E-cadherin genes and clinicopathological parameters, including HPV infection, phenotypic distribution, and stage of the disease. However, hypermethylation of E-cadherin gene promoter appears to be age related in cervical cancer, whereas the frequency of aberrant methylation of p16 INK4a gene promoter is unchanged according to the age of patients. Thus, caution must be made to use these markers in the diagnosis of cervical cancer. However, dietary or pharmaceutical agents that can inhibit these epigenetic events may prevent or delay the development of cervical cancer.

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“…HPV DNA was amplified by PCR using consensus primers MY09 and MY11, and HPV genotyping was realized by Southern blot hybridization using specific probes as described by Amrani et al (2).…”

Section: Hpv Detectionmentioning

confidence: 99%

“…Among the new cases estimated each year, approximately 80% occur in developing areas. In Morocco, cervical cancer is the second most common cancer among women and represents a major public health problem (2).…”

Section: Introductionmentioning

confidence: 99%

Status of p16<SUP>INK4a</SUP> and E-Cadherin Gene Promoter Methylation in Moroccan Patients With Cervical Carcinoma

Attaleb¹,

W²,

Khyatti³

et al. 2009

oncol res

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“…[3][4][5] We have therefore carried out a case-control study on the role of human papillomavirus (HPV) infection, as well as a broad range of lifestyle factors, in the etiology of CC in Algiers.…”

mentioning

confidence: 99%

Cervical carcinoma in Algiers, Algeria: Human papillomavirus and lifestyle risk factors

Hammouda¹,

Muñóz

Herrero

et al. 2004

Intl Journal of Cancer

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We conducted a hospital-based case-control study in Algiers, Algeria. A total of 198 cervical carcinoma (CC) cases (including 15 adeno-and adenosquamous carcinomas) and 202 age-matched control women were included. Human papillomavirus (HPV) DNA in cervical cells was evaluated using a PCR assay. Odds ratios and corresponding confidence intervals were computed by means of unconditional multiple logistic regression models. HPV infection was detected in 97.7% of CC cases and 12.4% of control women (OR ‫؍‬ 635). Nineteen different HPV types were found. HPV 16 was the most common type in both CC cases and control women,

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“…Previous studies in Moroccan women have been reported an inconsistent prevalence of HPV with 15.7% [6] in Rabat and 42.5-43.1% in Fez region [7,8]. However, the prevalence was much higher, ranging from 62% to 92%, among Moroccan women with CC [9][10][11][12]. The joint United Nations Programme on HIV/AIDS (UNAIDS) estimated that 30,000 (22,000 -40,000) Moroccans were living with HIV/AIDS at 2012, and women aged 15 years old and older living with HIV was 11,000 (8,500 -14,000).…”

Section: Introductionmentioning

confidence: 88%

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

Ouladlahsen¹,

Fayssel

Bensghir³

et al. 2018

J Infect Dev Ctries

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Introduction: Women infected with human immunodeficiency virus (HIV) have a higher risk of contracting human papillomavirus (HPV) infections and are more prone to develop cervical cancer. The objective of this study was to determine the prevalence of HPV and its association with risk factors among Moroccan women living with HIV/AIDS. Methodology: We enrolled 251 HIV-infected non-pregnant women in Morocco from February 2013 to September 2016. Sociodemographic, lifestyles, behavioral and clinical data were collected. Polymerase chain reaction followed by sequencing were performed for molecular detection and HPV genotyping in cervical samples, respectively. Results: Abnormal cervical smears were found in 34/246 patients (13.82%). The overall prevalence of HPV was 74.50%. HPV 58 was the most prevalent (39.29%) followed by HPV 18 (10.71%), HPV 70 (8.93%), HPV 33 (7.14%), HPV 6 (6.25%) and other genotypes (< 3%). Overall, high-risk HPV (HR-HPV) types were present in 75% (84/112) of patients and the prevalence of HR-HPV types in samples with abnormal Pap was higher than in normal Pap (55/83, 66.27% vs. 28/83,33.33%, p < 0.0001). Univariate analyses showed that none of the socio-demographic and behaviors factors was associated with HPV infection. Moreover, Pap results were not affected by HPV status (p = 0.532). Whereas, CD4 T-cell counts above 200/mm 3 at enrolment were apparently not protective to HPV infection. We found a high prevalence of HPV infection and HR-HPV types among HIV-positive women that significantly associated with abnormal Pap. Conclusion: Our findings suggest a high prevalence of HPV infection with high-risk types was observed among HIV-positive women warrant to implement a regular screening by Pap smear.

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Molecular detection of human papillomavirus in 594 uterine cervix samples from Moroccan women (147 biopsies and 447 swabs)

Cited by 20 publications

References 23 publications

Status of p16<SUP>INK4a</SUP> and E-Cadherin Gene Promoter Methylation in Moroccan Patients With Cervical Carcinoma

Status of p16<SUP>INK4a</SUP> and E-Cadherin Gene Promoter Methylation in Moroccan Patients With Cervical Carcinoma

Cervical carcinoma in Algiers, Algeria: Human papillomavirus and lifestyle risk factors

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

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