Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

Díaz-Regañón, David; Villaescusa, Alejandra; Ayllón, Tania; Rodríguez-Franco, Fernando; Baneth, Gad; Calleja-Bueno, Lydia; García-Sancho, Mercedes; Agulla, Beatriz; Sainz, Á.

doi:10.1186/s13071-017-2056-1

Cited by 59 publications

(77 citation statements)

References 44 publications

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“…In southern Italy, Hepatozoon spp.-DNA was detected in 5.1% (10/196) of the analysed cats; further sequencing of the obtained amplicons confirmed the presence of all three described Hepatozoon species affecting domestic cats: H. felis (n = 8), H. canis (n = 1) and H. silvestris (n = 1) [7]. In Spain, both H. felis (n = 9) and H. canis-DNA (n = 1) were detected by PCR/sequencing in blood from 1.6% (10/644) of cats in Madrid [14]. In the Barcelona area, H. felis was detected by PCR and sequencing in blood from 4% (4/100) [23] and 16% (4/25) [24] of cats.…”

Section: Discussionsupporting

confidence: 62%

“…lethargy, fever, weakness and lymphadenopathy), but also severe cases may occur [1,3,29]. In some prevalence studies, some or all cats with positive Hepatozoon PCR results in blood presented clinical signs that might have been associated with the infection [10][11][12]14,[23][24][25], while in other studies all positive cats were apparently asymptomatic [7].…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, in this study, only three H. felis gamonts were observed in the whole blood smear, and no other pathogens could be microscopically detected. Noteworthy, most prevalence studies on feline hepatozoonosis were exclusively based on molecular techniques [7,[10][11][12]14,23,25] and the presence of circulating gamonts was only rarely reported [1,34,36].…”

Section: Discussionmentioning

confidence: 99%

“…So far, three different Hepatozoon species were described in domestic cats in Europe: H. felis, H. canis and H. silvestris [1,7,8]. H. felis has been the most frequently diagnosed species in cases of feline hepatozoonosis in different countries around the world [1,[9][10][11][12][13][14]. Interestingly, while some Hepatozoon species seem to be hostspecific, H. canis, the worldwide distributed Hepatozoon species infecting domestic dogs, is also able to infect cats [3,7,15,16] and several species of wild carnivores [17][18][19][20][21][22], which may represent an infection reservoir for domestic animals.…”

Section: Introductionmentioning

confidence: 99%

“…In Europe, H. felis-DNA was identified by PCR in blood from domestic cats from Italy [7]; Spain [14,23,24]; Portugal [10,11] and Cyprus [9,12]. H. canis-DNA was detected in blood from cats in Italy [7]; Spain [14,16] and France [25]. H. silvestris, the most recently described species, was first detected in European wild cats (Felis silvestris silvestris) from Bosnia and Herzegovina in 2017 [2].…”

Section: Introductionmentioning

confidence: 99%

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First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

Basso

Görner²,

Globokar

et al. 2019

Parasitology International

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A B S T R A C TThree different Hepatozoon (Apicomplexa, Hepatozoidae) species have been described infecting domestic cats in Europe (i.e. H. felis, H. canis and H. silvestris), however, reports on clinical hepatozoonosis are uncommon and treatment protocols are not clearly defined.A six-year-old male European short-hair cat from Austria presented poor general condition, lethargy, anorexia, icterus, a painful abdomen, fever, ruffled hair and a tick infestation, and it had never left Austria. Laboratory tests revealed leukopenia, thrombocytopenia and increased serum levels of symmetric dimethylarginine (SDMA) and bilirubin. In May Grünwald-Giemsa-stained blood smears, structures resembling Hepatozoon gamonts were observed inside neutrophil granulocytes. A PCR targeting a fragment of the 18S rRNA gene of Hepatozoon spp. and DNA sequencing allowed the diagnosis of H. felis-DNA in blood samples. The cat was treated with imidocarb dipropionate (6 mg/kg body weight, repeated after 14 days) and doxycycline monohydrate (5 mg/kg body weight twice a day, p.o., for four weeks) and recovered completely. A broad haematological and biochemical laboratory control after six months showed all evaluated parameters under normal ranges. Coinfection with other feline pathogens (i.e. feline leukaemia virus, feline immunodeficiency virus, feline Coronavirus, Leishmania and Dirofilaria immitis) could not be detected.This study reveals the presence of H. felis in Austria and provides more evidence on the geographical distribution and pathogenicity of this parasite for domestic cats. To the authors' knowledge, this is the first autochthonous case of feline hepatozoonosis in Central Europe.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

Basso

Görner²,

Globokar

et al. 2019

Parasitology International

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First report of Cytauxzoon sp. infection in Germany: organism description and molecular confirmation in a domestic cat

et al. 2020

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Cytauxzoonosis is described as an emerging tick-borne disease of domestic and wild felids caused by protozoans of the genus Cytauxzoon . While in the Americas the condition is described as a fatal disease, in Europe, reports on the clinical expression of the infection are scarce. This study describes the first case of Cytauxzoon sp. infection in Germany, in a domestic cat. A 6-year-old male domestic cat living in Saarlouis (Saarland) was presented with anorexia, lethargy and weight loss. The cat had an outdoor lifestyle and had not travelled abroad. Serum clinical chemistry analysis revealed azotaemia with markedly increased symmetric dimethylarginine, hypercreatinemia, hyperphosphatemia and hypoalbuminemia. Moreover, a mild non-regenerative anaemia was present. Approximately 1 year prior to these findings, the domestic cat was diagnosed with a feline immunodeficiency virus (FIV) infection. These results pointed toward a decreased glomerular filtration rate, presumably as a result of kidney dysfunction. Round to oval signet ring–shaped intraerythrocytic organisms, morphologically suggestive for a piroplasm, were revealed during blood smear evaluation with a degree of parasitaemia of 33.0%. PCR analyses and sequencing of a region of the 18S rRNA gene confirmed the presence of a Cytauxzoon sp. infection, with 99–100% nucleotide sequence identity with previously published Cytauxzoon sp. isolates. As this is the first molecularly confirmed Cytauxzoon sp. infection in a domestic cat in Germany, these findings suggest that cytauxzoonosis should be considered as a differential diagnosis in cases of anaemia in outdoor domestic cats, particularly in areas where wild felid populations are present.

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Piroplasmid infection is not associated with clinicopathological and laboratory abnormalities in cats from Midwestern Brazil

Oliveira

Yang²,

Duarte

et al. 2022

Parasitol Res

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Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

Cited by 59 publications

References 44 publications

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

First report of Cytauxzoon sp. infection in Germany: organism description and molecular confirmation in a domestic cat

Piroplasmid infection is not associated with clinicopathological and laboratory abnormalities in cats from Midwestern Brazil

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