Molecular detection of Hematodinium sp. in Northeast Pacific Chionoecetes spp. and evidence of two species in the Northern Hemisphere

Jensen, Pamela C.; Califf, Katy J.; Lowe, Vanessa; Hauser, Lorenz; Morado, J. Frank

doi:10.3354/dao02193

Cited by 35 publications

(52 citation statements)

References 49 publications

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“…ITS1 sequences from L. depurator were, on the other hand, very similar to sequences from Callinectes sapidus (i.e. Clade A, Jensen et al 2010). In other words, representatives from both Hematodinium clades may be able to infect L. depurator.…”

Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 71%

“…EU096208, Table 3). Furthermore, Jensen et al (2010) found a sequence FJ844426 from C. angulatus from the NW Atlantic that is identical to sequences from the NE Atlantic subclade (e.g. EF675761, Table 3).…”

Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 97%

“…5, sequence number 2, and Table 3) derived from Chionoecetes opilio are affiliated with Clade B (Jensen et al 2010). It is noteworthy that these 2 sequences were very similar even though they originated from 2 different host crustaceans, and it is, therefore, unlikely that these 2 aberrant sequences were results of methodological artefacts.…”

Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 98%

“…Different Hematodinium genotypes were related to different host species (Small et al 2007b, Hamilton et al 2010, Jensen et al 2010) and geographical regions (Hamilton et al 2010). It is also clear, however, that a single Hematodinium genotype may infect more than one host species (Hamilton et al 2007, Small et al 2007a, Jensen et al 2010.…”

Section: Introductionmentioning

confidence: 99%

“…Sequence similarities were calculated as percentages of identical bases. Evolutionary distances between ITS1 sequences obtained in the present study and sequences taken from GenBank (Clade B as defined by Jensen et al 2010, and sequences from each subclade defined by Hamilton et al 2010) were estimated with an alignment of 13 sequences (4 obtained in the present study and 9 taken from GenBank) using MEGA4 (Tamura et al 2007), whereby positions containing gaps were eliminated from the data set. There were a total of 289 positions in the final alignment (excluding gap-containing positions).…”

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confidence: 99%

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Hematodinium sp. (Alveolata, Syndinea) detected in marine decapod crustaceans from waters of Denmark and Greenland

Eigemann

Burmeister²,

Skovgaard³

2010

Dis. Aquat. Org.

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Five decapod crustacean species were examined for presence of the parasitic dinoflagellate Hematodinium spp. (Alveolata, Syndinea) by morphological methods (colour and pleopod methods) as well as by PCR and nested PCR with Hematodinium-specific primers. Nephrops norvegicus, Pagurus bernhardus and Liocarcinus depurator were sampled by trawling in Danish waters and Chionoecetes opilio and Hyas araneus were sampled by trapping off the west coast of Greenland. The existence of Hematodinium has not previoiusly been documented in Danish waters, but it was detected in all 3 decapod species examined in the present study. Hematodinium sp. was also detected for the first time in H. araneus and the existence of Hematodinium sp. in Greenlandic C. opilio was documented by PCR. Analyses of 26 Hematodinium sp. ITS1 sequences, including sequences from all 5 host species sampled, revealed more than 95% sequence similarity between 24 of the sequences. Two Hematodinium sp. ITS1 sequences from C. opilio were only 81% similar to the 24 other ITS1 sequences. The nested PCR approach resulted in the highest reported percentages of positive samples for Hematodinium sp.in the hosts investigated (between 45 and 87.5%). However, no decapods were found to be infected with Hematodinium sp. based on morphological methods. Consequently, Hematodinium sp. may be more common than previously believed, and, assuming that the DNA found originated from viable and infectious parasite cells, infections may not always be fatal. We suggest that the hosts investigated may have been subject to latent infections that could develop into a fatal disease only if the hosts were physiologically stressed due to other factors. KEY WORDS: Hematodinium · Latent infections · ITS1 · Parasitic dinoflagellates · PCR detection Resale or republication not permitted without written consent of the publisherDis Aquat Org 92: [59][60][61][62][63][64][65][66][67][68] 2010 dinium sequences may warrant species status (Small et al. 2007b,c, Jensen et al. 2010. Different Hematodinium genotypes were related to different host species (Small et al. 2007b, Hamilton et al. 2010, Jensen et al. 2010) and geographical regions (Hamilton et al. 2010). It is also clear, however, that a single Hematodinium genotype may infect more than one host species (Hamilton et al. 2007, Small et al. 2007a, Jensen et al. 2010. However, so far only one other species of Hematodinium has been formally described, namely H. australis, which was described on the basis of morphology, host species, and austral location (Hudson & Shields 1994).In some host species, advanced infections lead to a discoloration of the carapace from reddish-brown to a reddish 'cooked' appearance (e.g. in Nephrops norvegicus and Chionoecetes opilio) and this may be used for detection of the disease (colour method; Meyers et al. 1987, Field et al. 1992. Rapid logarithmic growth of parasitic cells in the host's haemolymph causes a colour change from bluish-transparent to milky-white (e.g. Shields & Squyars 2000) and aggregat...

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Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 71%

Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 97%

Section: Sequence Comparison and Evolutionary Divergencementioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Hematodinium sp. (Alveolata, Syndinea) detected in marine decapod crustaceans from waters of Denmark and Greenland

Eigemann

Burmeister²,

Skovgaard³

2010

Dis. Aquat. Org.

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Lower Slope and Abyssal Benthic Decapods of the Eastern Pacific

Wicksten

2020

Deep-Sea Pycnogonids and Crustaceans of the Americas

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Parasite–copepod interactions in Svalbard: diversity, host specificity, and seasonal patterns

et al. 2022

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Copepods of the genera Calanus and Pseudocalanus are important components of Arctic marine ecosystems. Despite the key roles of these zooplankters, little is known about the organisms they interact with most intimately, their parasites and symbionts. We applied metabarcode sequencing to uncover eukaryotic parasites present within these two copepod genera from three areas around the high Arctic archipelago of Svalbard. Ten distinct parasite groups were observed: four different Apostome ciliates, four different dinoflagellates (Chytriodinium sp., Ellobiopsis sp., Thalassomyces sp., and Hematodinium sp.), a Paradinium sp., and a trematode. Apostome ciliates closely related to Pseudocollinia spp. were the most commonly observed parasite, with overall infection rates of 21.5% in Calanus and 12.5% in Pseudocalanus. Infection by these ciliates varied seasonally, with no infections observed in early winter, but infection rates exceeding 75% in spring. Host specificity varied between parasites, with significant differences in infection rate between the two host copepod genera for four parasites (two ciliates, Chytriodinium, and a trematode). The diverse assemblage of parasites observed in these copepods, and the frequency of infection, with over one in five copepod individuals infected, suggest parasites may be playing a greater role in Arctic plankton communities than generally acknowledged.

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Molecular detection of Hematodinium sp. in Northeast Pacific Chionoecetes spp. and evidence of two species in the Northern Hemisphere

Cited by 35 publications

References 49 publications

Hematodinium sp. (Alveolata, Syndinea) detected in marine decapod crustaceans from waters of Denmark and Greenland

Hematodinium sp. (Alveolata, Syndinea) detected in marine decapod crustaceans from waters of Denmark and Greenland

Lower Slope and Abyssal Benthic Decapods of the Eastern Pacific

Parasite–copepod interactions in Svalbard: diversity, host specificity, and seasonal patterns

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