Molecular Detection of Glycophorins A and B Variant Phenotypes and their Clinical Relevance

Hassan, Siti Nazihahasma; Kannan, Thirumulu Ponnuraj; Mohamad, Suharni; Hassan, Rosline; Rahman, Wan Suriana Wan Ab

doi:10.1016/j.tmrv.2019.02.003

Cited by 8 publications

(8 citation statements)

References 68 publications

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“…Its antigens include sialic acid glycoproteins, such as GPA and GPB, which are connected to proteins through O-and N-. But there are very few studies on the relationship between sugar chain differences and antigenicity [13][14] .…”

Section: Rhd Exonsmentioning

confidence: 99%

Overview of the diversity of erythrocyte blood group antigens

Yang¹,

Feng²

2020

Blood and Genomics

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During the differentiation and maturation of erythrocytes, the surface molecules of erythrocytes are gradually expressed and stabilized. These molecules are to be antigenic in addition to their functions of maintaining cell membrane structural stability, material transport and exchange of cells and signal transmission between cells. The antigenic molecules on the erythrocyte surface are called erythrocyte blood group antigens. The blood group antigens and their corresponding blood group antibodies in vivo are important indicators for clinical blood transfusion and organ transplantation, and also form the basis for research on blood group related diseases. Three hundred and sixty-eight erythrocyte blood group antigens have been confirmed so far, which are classified into 39 blood group systems, 5 blood group collections and 2 blood group series. Based on the diversity of blood group antigens and their composition of glycolipids, glycoproteins and other molecules, this study mainly reviews the classification, molecular structure, antibody response and gene regulation of blood group antigens, and explains the main reasons for the diversity of blood group antigens.

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Section: Rhd Exonsmentioning

confidence: 99%

Overview of the diversity of erythrocyte blood group antigens

Yang¹,

Feng²

2020

Blood and Genomics

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“…Su función principal es la determinación antigénica para los grupos sanguíneos MN y Ss; así mismo, está involucrada en la interacción entre los GR y el endotelio vascular (24).…”

Section: Receptores Que Interactúan Con Proteínas De Los Micronemas De P Falciparum Glicoforina Aunclassified

Receptores del hospedero implicados en la invasión del merozoito de Plasmodium falciparum: Revisión

Franky

Rodríguez

Santofimio

et al. 2019

Revista Investig. Salud Univ. Boyacá

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Introducción. La malaria es un problema de salud pública a nivel mundial y es causada por 5 especies de parásitos apicomplejos del género Plasmodium. La invasión exitosa de los merozoítos al glóbulo rojo es una etapa fundamental en el ciclo de vida del parásito, el cual usa un variado repertorio de ligandos que interactúan de forma específica con receptores presentes en la membrana del glóbulo rojo. Objetivo. Revisar las características moleculares y estructurales de los receptores expresados en la superficie de los glóbulos rojos, implicados en el proceso de invasión del merozoito de Plasmodium falciparum. Método. Revisión descriptiva sobre las características moleculares y estructurales de los receptores de la superficie del glóbulo rojo, los cuales juegan un papel fundamental durante la invasión del merozoíto de Plasmodium falciparum. Esta revisión empezó por la búsqueda de literatura publicada hasta el año 2019 en bases de datos electrónicas, especializadas en la divulgación de investigación biomédica. Se encontraron 127 documentos, de los cuales se seleccionaron 111 y se excluyeron 33 por no cumplir los criterios de inclusión; en total, se analizaron 78 referencias. Conclusión. En esta revisión se resumieron las características moleculares y estructurales de los receptores presentes en el glóbulo rojo importantes en el proceso de invasión del merozoito de P. falciparum. También, se resaltó la importancia de elucidar las diferentes vías de invasión del parásito y así, poder desarrollar alternativas profilácticas o terapéuticas que conduzcan a mitigar o eliminar la malaria

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“…MNS antigens are carried on two sugar-bearing proteins, glycophorin A (GPA) and glycophorin B (GPB), which are encoded by GYPA and GYPB genes respectively. At present, 49 MNS antigens have been recognized [6]. Among them, only M, N, S and s antigens, of which the composition and structure are determined by the sequences in original GYPA and GYPB genes, are regarded as regular antigens, whereas the others are MNS-related variant antigens as their features cannot be extrapolated on the basis of standard sequences of GYPA and GYPB genes.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, novel MNS-related blood group antigens are identified mainly via genotype-based screening of genomic DNA for hybrid glycophorin genes, for example, GYP(A-B), GYP(B-A-B) etc. [6,14], whereas glycophorin transcripts (mRNA) are seldom examined, possibly due to the instability of RNA and technical challenges in RNA manipulation. However, it was reported that accuracy rate of DNA sequencing-based diagnosis, either by whole exome sequencing or whole genome sequencing, is less than 50% for Mendelian diseases whereas RNA sequencing provided a substantial increase in diagnosis rate, indicating information provided by RNA analysis was necessary for phenotype prediction in addition to DNA analysis [15].…”

Section: Introductionmentioning

confidence: 99%

Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors

et al. 2022

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Background and Objectives The molecular basis of MNS blood group variants is not fully clear yet. In this study, we have characterized mRNA variants of GYPA and GYPB genes to reveal whether alternative RNA splicing may cause antigenic diversity of the MNS system. Materials and Methods Total RNA was extracted from peripheral blood of Chinese blood donors and full‐length cDNA products were generated. A nested polymerase chain reaction (PCR)‐based method was established for fragment amplification and Sanger sequencing. Resulted full‐length mRNA sequences were aligned with GYPA or GYPB genomic sequences respectively for exon identification. Amino acid (AA) sequences of GPA and GPB proteins were extrapolated and GYPA‐EGFP, GYPB‐EGFP fusion genes were generated to monitor subcellular distribution of the encoded glycophorin (GP) proteins. Results Totally 10 blood samples were analysed. GYPB mRNAs of all the subjects demonstrated frequent exon insertion or deletion whereas this kind of variation was only observed in 3 of 10 GYPA mRNA samples. None of the reported Miltenberger hybrids was detected in any of the mRNA samples. The alternative splicing resulted in changes of AA sequences in N‐terminal domains where the MNS antigenic motifs resided; however, subcellular localizations of GP‐EGFP fusion proteins showed that the above‐mentioned AA changes did not affect cell surface distribution of the encoded GP proteins. Conclusions Alternative RNA splicing may influence the antigenic features of GP proteins but not their cell surface distribution. Therefore, GYPA and GYPB mRNA characterization might be an invaluable supplement to serological phenotyping and DNA‐based genotyping in MNS blood grouping.

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Molecular Detection of Glycophorins A and B Variant Phenotypes and their Clinical Relevance

Cited by 8 publications

References 68 publications

Overview of the diversity of erythrocyte blood group antigens

Overview of the diversity of erythrocyte blood group antigens

Receptores del hospedero implicados en la invasión del merozoito de Plasmodium falciparum: Revisión

Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors

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