Molecular detection of extended spectrum β-lactamases, metallo β-lactamases, and Amp-Cβ-lactamase genes expressed by multiple drug resistant Pseudomonas aeruginosa isolates collected from patients with burn/wound infections

Nasser, Mahfouz; Ogaili, Mahmoud; Palwe, Snehal; Kharat, Arun S.

doi:10.1016/j.burnso.2020.07.003

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…SIM and GIM were detected in 26 (39%) and 20 (37%) isolates respectively. These three enzymes predominate the metallo-beta-lactamases in our isolates, but the rate is comparable with other studies that report a dominance of VIM ( 33 ).…”

Section: Discussionsupporting

confidence: 92%

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

Massik¹,

Hibaoui²,

Benboubker³

et al. 2022

IJM

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Background and Objectives: Carbapenem-resistant Acinetobacter baumannii has recently been identified by the World Health Organization as a critical pathogen. We propose to characterize the molecular characteristics of clinical isolates of A. baumannii resistant to carbapenems collected in a Moroccan hospital. Materials and Methods: Seventy carbapenem-resistant A. baumannii isolates from various samples were received at the mi- crobiology laboratory of the Hospital Center. Antibiotic susceptibility was tested by the diffusion disc method and molecular characterization of antimicrobial resistance was performed by PCR and sequencing. Results: Carbapenemase genes were detected in our isolates: the OXA-51 gene and the ISbA1 sequence were detected in all isolates (100%), the OXA-23 and OXA-58 genes were detected in 82.85% and 10% of isolates respectively, MBL genes were dominated by VIM 39 isolates (55.7%), followed by GIM 26 isolates (37%), SIM 20 isolates (28.5%), IMP 8 isolates (11, 4%), NDM 3 isolates (4%) and for the first time in Morocco SPM with 4 isolates (5.7%). Conclusion: The emergence of resistance of A. baumannii to carbapenems is a serious problem in our hospital which re- quires the establishment of a prevention strategy and strict respect for hygiene to minimize their dissemination.

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Section: Discussionsupporting

confidence: 92%

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

Massik¹,

Hibaoui²,

Benboubker³

et al. 2022

IJM

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“…In another study, resistance to most beta-lactam antibiotics was higher in bla VEB gene-positive P. aeruginosa isolates than in isolates harboring bla PER and bla GES genes [38] . Moreover, Nasser et al (2020) showed a high frequency of bla VEB and bla GES genes in MDR P. aeruginosa isolates compared to other ESBL genes [43] . The higher incidence of MDR in P. aeruginosa isolates harboring the bla VEB gene may be explained by the fact that this gene is carried on plasmids containing other resistant genes.…”

Section: Discussionmentioning

confidence: 99%

High Incidence of MDR and XDR Pseudomonas aeruginosa Isolates Harboring blaGES, blaVEB, and blaPER Extended-Spectrum Beta-Lactamase Genes in Iran

Beiki

Kareshk²,

Askari³

et al. 2022

IEM

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Backgrounds:The ever-increasing incidence of multidrug resistance in ESBL-producing Pseudomonas aeruginosa is one of the most serious public health threats. This study aimed to investigate the antibiotic resistance profile and molecular characteristics of ESBL-producing P. aeruginosa isolates. Materials & Methods: Antimicrobial susceptibility testing was performed for 120 P. aeruginosa clinical isolates using the Kirby-Bauer disk diffusion and broth microdilution assays. Combined disk test (CDT) was applied to screen for ESBL production among P. aeruginosa isolates. PCR assays determined the presence of bla GES , bla PER , and bla VEB genes in all isolates. Findings: The clinical isolates of P. aeruginosa showed the highest resistance to cefotaxime (86.7%) and gentamicin (65.8%). Of 120 P. aeruginosa isolates, 60.8% were MDR, and 53.3% were XDR. The prevalence of these strains was significantly higher in hospitalized patients than in out-patients (p<.001). Also, 58 P. aeruginosa strains (48.3%) were considered as phenotypic ESBL producers. Furthermore, 15, 35, and 24.2% of P. aeruginosa isolates harbored bla GES , bla VEB , and bla PER , respectively. The incidence of MDR (71.4% vs. 41.9%, p= .001) and XDR (63.6% vs. 34.9%, p= .002) was significantly higher in ESBL-producing P. aeruginosa isolates compared to non-ESBL producers. The highest incidence rate of MDR was reported in bla VEB gene-positive P. aeruginosa isolates (95.2%), followed by isolates harboring bla PER (79.3%) and bla GES (55.6%) genes. Conclusion:This study findings show a high prevalence of MDR ESBL-producing P. aeruginosa isolates, indicating the importance of correct identification of these superbugs and judicious use of various antibiotics to prevent their spread.

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“…The studies are less on the molecular screening of ampC gene mutation in P. aeruginosa. A recent study done by Nasser et al (2020) showed that the prevalence rate of ampC gene as 61.5%, 68.6% in Iran and 36.8% from China.…”

Section: Inducible Ampc Beta-lactamasesmentioning

confidence: 99%

Antimicrobial resistance in Pseudomonas aeruginosa

Mohanam¹,

Shanmugam²

2022

MJM

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In recent years, antimicrobial resistance (AMR) has become a global public health concern. The growth of resistant bacteria is increasing dramatically, while the number of new antibiotics accessible is decreasing. This is especially true in the case of Pseudomonas aeruginosa, an important causative agent of healthcare-associated infections. The ability of P. aeruginosa to survive in different environments and on medical devices has made it more resistant to antibiotics. This causes bacteremia in hospitalized patients, ventilator-associated pneumonia, catheter-associated urinary tract infections and wound infections, particularly in patients with severe burns, bed ulcers and immunocompromised individuals. The rise in the AMR rate in both developed and developing countries may be attributed to a number of factors such as variations in the standard health care, large population, awareness about antibiotic resistance, inadequate training on rationale antibiotic usage and inadequate infection control facilities in many hospitals. The emergence of Extensive Drug Resistance (XDR) and Pan Drug Resistance (PDR) among organisms that cause various infections leads to increased treatment costs, morbidity and mortality, leaving no therapeutic options. This review highlights the different mechanisms of antibiotic resistance, including intrinsic and acquired resistance, which are frequently observed in P. aeruginosa.

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Molecular detection of extended spectrum β-lactamases, metallo β-lactamases, and Amp-Cβ-lactamase genes expressed by multiple drug resistant Pseudomonas aeruginosa isolates collected from patients with burn/wound infections

Cited by 10 publications

References 31 publications

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

First report of SPM metallo-β-lactamases producing Acinetobacter baumannii isolates in Morocco

High Incidence of MDR and XDR Pseudomonas aeruginosa Isolates Harboring blaGES, blaVEB, and blaPER Extended-Spectrum Beta-Lactamase Genes in Iran

Antimicrobial resistance in Pseudomonas aeruginosa

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