Molecular Detection of Diverse Mycoplasmalike Organisms (MLOs) Associated with Grapevine Yellows and Their Classification with Aster Yellows, X-Disease, and Elm Yellows MLOs

Prince, James P.; Davis, R. E.; Wolf, Tony K.; Lee, Ing‐Ming; Mogen, Bradley D.; Dally, E. L.; Bertaccini, Assunta; Credi, R.; Barba, M.

doi:10.1094/phyto-83-1130

Cited by 147 publications

(97 citation statements)

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“…The causal agent was transmitted to periwinkle [Catharanthus roseus (L.) G. Don] by graft inoculation and the infected periwinkles were used as samples. Hydrangea phyllody phytoplasma isolates other than JHP phytoplasma used for RFLP analyses were an Italian isolate (HyPhl) (Lee et al, 1993), Belgium isolate (HYDP) (Schneider et al, 1993), French isolate (HF) (Cousin & Sharma, 1986), and three Canadian isolates (HC) (Hiruki et al, 1994). Additional samples were taken from healthy, greenhouse-grown hortensia and periwinkle seedlings.…”

Section: Methodsmentioning

confidence: 99%

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‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody

Sawayanagi

Horikoshi

Kanehira

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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A phytoplasma was discovered in diseased specimens of f ield-grown hortensia (Hydrangea spp.) exhibiting typical phyllody symptoms. PCR amplification of DNA using phytoplasma specific primers detected phytoplasma DNA in all of the diseased plants examined. No phytoplasma DNA was found in healthy hortensia seedlings. RFLP patterns of amplified 165 rDNA differed from the patterns previously described for other phytoplasmas including six isolates of foreign hortensia phytoplasmas. Based on the RFLP, the Japanese Hydrangea phyllody (JHP) phytoplasma was classified as a representative of a new subgroup in the phytoplasma 165 rRNA group I (aster yellows, onion yellows, all of the previously reported hortensia phytoplasmas, and related phytoplasmas). A phylogenetic analysis of 16s rRNA gene sequences from this and other group I phytoplasmas identified the JHP phytoplasma as a member of a distinct sub-group (sub-group Id) in the phytoplasma clade of the class Mollicutes. The phylogenetic tree constructed from 165 rRNA gene sequences was consistent with the hypothesis that the JHP phytoplasma and its closest known relatives, the Australian grapevine yellows (AUSGY), Phormium yellow leaf (PYL), Stolbur of Capsicum annuum (STOL) and Vergilbungskrankheit of grapevine (VK) share a common ancestor. The unique properties of the DNA from the JHP phytoplasma clearly establish that it represents a new taxon, Candidatus Phytoplasma japonicum '.

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Section: Methodsmentioning

confidence: 99%

“…The RFLP patterns of JHP phytoplasma DNA were compared with those of amplified DNA from the reference strains and previously published data (Ahrens & Seemiiller, 1992;Lee et al, 1993;Prince et al, 1993;Schneider et al, 1993). The group names 'I'-'V' were followed from the previous report (Seemiiller et al, 1994).…”

Section: Methodsmentioning

confidence: 99%

‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody

Sawayanagi

Horikoshi

Kanehira

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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“…Phytoplasma asteris', 'Ca. Phytoplasma pruni' (Prince et al 1993), and 'Ca. Phytoplasma fraxini' (Gajardo et al 2009).…”

Section: Introductionmentioning

confidence: 99%

‘Bois noir’: new phytoplasma disease of grapevine in Iran

Mirchenari¹,

Massah²,

Zirak³

2015

Journal of Plant Protection Research

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Abstract:Recently, grapevines showing symptoms suggesting the 'bois noir' phytoplasma disease were observed in vineyards located in several central provinces of Iran. Polymerase chain reaction assays using phytoplasma universal primer pair P1A/P7A followed by primer pair R16F2n/R16R2 in nested PCR, confirmed the association of phytoplasmas with symptomatic grapevines. The results of RFLP analyses using HpaII, HinfI, MseI, RsaI, and TaqI restriction enzymes, indicated that grapevine phytoplasma isolates in these regions could be related to the 16SrXII group. Sequence analyses of the partial 16S rRNA gene confirmed that Iranian grapevine phytoplasmas are associated with 'Candidatus Phytoplasma solani'. This is the first report of the 'bois noir' disease outbreak in Iran.

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“…Insect vectors can acquire more than one phytoplasma species either by feeding on multiple-infected source plants or by feeding on different plants infected by different phytoplasmas (8,9,16). Phytoplasma infections can only be identified by PCR and nested-PCR, followed by PCR-RFLP according to the ribosomal 16Sr RNA gene by using general and group specific primers (24,25,34).…”

mentioning

confidence: 99%

“…Nucleic acids were extracted from midribs of the infected plants leaves according to chloroform/phenol protocol (34). Direct PCR with ribosomal P1/P7 universal primer pair, followed by nested PCR with R16F2n/R2, R16 (I)F1/R1 and then with R16(V) F1/VR1 primer pairs were carried out.…”

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confidence: 99%

A new threat for Turkish horticulture: phytoplasma diseases and their vectors

Ertunç¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Our country is one of the nations settled in temperate region which enables it high agricultural potential.Agriculture is done as viticulture, field's crops, variable fruits, vegetables and ornamental crops production and constitutes great impact on Turkish economy. Plant diseases are one of the important factors which limit the production of all those crops. Recently phytoplasma diseases are the most important agents that limit the agricultural production in Turkey and in the world. Transmission by vegetative plant material and also by vectors causes epidemics in very short period. Stolbur on potato and tomato, Apple proliferation and Pear decline on pomes fruits, European stone fruit yellows on stone fruits, Flavescense doree, Bois Noir on vineyards are the most important phytoplasma infections in our country. AP, PD and ESFY are transmitted by the vector species belonging to Hemiptera order, Psyllidae family whereas FD and BN are transmitted by the species of Cicadellidae, Cixiidae families.Key words: Phytoplasma, vector, Hemiptera. Türk bahçeciliğinde yeni bir tehdit: fitoplazma hastalıkları ve vektörleriÖzet: Türkiye ılıman iklim kuşağında yer alan ve bu nedenle tarım potansiyeli yüksek ender ülkelerden biridir. Ülkemizde tarım, tarla bitkileri, bağ, çeşitli meyve, sebze ve süs bitkilerinin tarımı şeklinde yapılmakta olup, ülke ekonomisinde önemli yere sahiptir. Tüm bu ürünlerde üretimi kısıtlayan en önemli unsurlardan biri de bitkilerde görülen hastalıklardır. Fitoplazma hastalıkları da son yıllarda ülkemiz ve dünya tarımında üretimi kısıtlayan en önemli etmenleri oluşturmaktadır. Vegetatif olarak üretim materyali ile ayrıca vektör böceklerle kolaylıkla yayılmaları, kısa sürede epidemi oluşturmalarına neden olmaktadır. Patates ve domateste stolbur, yumuşak çekirdekli meyvelerde gözlenen Apple proliferation (AP), Pear Decline (PD), sert çekirdekli meyve türlerinde görülen European stone fruit yellows (ESFY), bağlarda görülen Flavescense doree (FD) and Bois Noir (BN) hastalıkları ülkemizde üretimi kısıtlayan en etkin enfeksiyonlardır. AP, PD ve ESFY enfeksiyonları Hemiptera takımında yer alan ve Psyllidae, FD ve BN hastalıkları ise Cicadellidae, Cixiidae familyasında yer alan türlerle taşınmaktadır.

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Molecular Detection of Diverse Mycoplasmalike Organisms (MLOs) Associated with Grapevine Yellows and Their Classification with Aster Yellows, X-Disease, and Elm Yellows MLOs

Cited by 147 publications

References 0 publications

‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody

‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody

‘Bois noir’: new phytoplasma disease of grapevine in Iran

A new threat for Turkish horticulture: phytoplasma diseases and their vectors

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