Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars

Kumar, Santosh; Srivastava, Andarun; Raj, S. K.

doi:10.1556/aphyt.40.2005.3-4.2

Cited by 10 publications

(2 citation statements)

References 4 publications

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“…Deoxy ribonucleic acid sequencing is the process of determining the molecular sequence of particular gene, which determines the order of the four nitrogen bases viz., adenine, guanine, cytosine, and thymine.The sequence of the CP gene of the CMV from paprika (Capsicum annuum L.) contained a single open reading frame of 657 nucleotides potentially coding for 218 amino acids (Bhadramurthy et al, 2009). Indian isolates of CMV, infecting various crops were sequenced and found out its homology with subgroup II of CMV (Kumar et al, 2005;Kumari et al, 2013). High sequence identities and evolutionary tie in coat protein gene has been observed with CMV isolate fromKerala (Mujtaba, 2017).…”

Section: Molecular Detection Of Virus Variabilitymentioning

confidence: 99%

The Virus Causing Infectious Chlorosis in Banana (Musa sp.): A Review

Antony¹,

Shaana²,

Visakh³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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Banana fruit crops are high in potassium, contain a high level of protein and dietary fiber which makes it world's most popular. The crop gives a maximum return to the farmers when good cultivation practices are followed, which include using diseasefree planting material. The crop is infected by several viruses viz., Banana bunchy top virus, Cucumber mosaic virus, Banana streak virus, Banana bract mosaic virus and Banana mild mosaic virus (Tripathi et al., 2016). Among these viruses, CMV causes a devastating effect on tissue culture banana plants. Various symptoms of CMV were reported under natural conditions like diamond-shaped discontinuous lesions, severe mosaic with extreme distortion and reduction of leaf lamina. KAU (2016) opined that infection due to CMV was observed in widely cultivated banana varieties in Kerala, such as Nendran, Palyankodan, Karpooravally and Poovan (Rasthali). Hence, virus indexing is a must to select healthy planting materials. Cucumber mosaic virus is transmitted through more than 60 species of aphids, including Aphis gossypii and Myzuspersicaeetc. The serological methods are widely used in the detection of Cucumber mosaic virus from the field.This review paper is focused on various aspects of novel detection methods of CMV infecting banana.

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Section: Molecular Detection Of Virus Variabilitymentioning

confidence: 99%

The Virus Causing Infectious Chlorosis in Banana (Musa sp.): A Review

Antony¹,

Shaana²,

Visakh³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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“…The occurrence of CMV subgroup II strains is rare in India, except for a few reports (Sudhakar et al 2006;Verma et al 2006). Usually, CMV strains occurring in India belong to subgroup IB (Kumar et al 2005;Pratap et al 2008;Samad et al 2008;Srivastava and Raj 2004).…”

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confidence: 99%

Molecular identification of a new isolate of Cucumber mosaic virus subgroup II from basil (Ocimum sanctum) in India

Khan¹,

Sharma

Afreen

et al. 2011

Phytoparasitica

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Natural occurrence of mosaic disease was observed on basil (Ocimum sanctum L.) in Aligarh, U. P., India, during 2008. The disease could be transmitted by sap inoculations from naturally infected O. sanctum to O. sanctum and some test plant species. Cucumber mosaic virus (CMV) was detected by RT-PCR using coat protein gene specific primers of CMV (Acc. AM180922 & AM180923), which resulted in the expected size~650 bp amplicon in infected samples. The amplicon was cloned, sequenced and data were deposited in GenBank Acc. EU600216. The sequence data analysis revealed 97-99% identities at both nucleotide and amino acid levels with the CMV strains of subgroup II reported worldwide. Based on the high sequence identities and close phylogenetic relationships with CMV subgroup II strains, the virus under study has been identified as a new isolate of CMV subgroup II and designated as CMV-Basil.

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