Molecular detection of Cryptosporidium spp. and the occurrence of intestinal parasites in fecal samples of naturally infected dogs and cats

Alves, Marta Elena Machado; Martins, Felippe Danyel Cardoso; Bräunig, Patrícia; Pivoto, Felipe Lamberti; Sangioni, Luís Antonio; Vogel, Fernanda Silveira Flores

doi:10.1007/s00436-018-5986-4

Cited by 28 publications

(22 citation statements)

References 39 publications

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“…The rate of positive samples is in agreement with other studies conducted in Brazil by only parasitological techniques (Coelho et al, 2009;Funada et al, 2007;Gennari et al, 1999;Huber, Silva, Bomfim, Teixeira, & Bello, 2007;Moreira et al, 2018;Pivoto, Lopes, Vogel, Botton, & Sangioni, 2013;Ragozo et al, 2002). The molecular characterization was performed in just a few studies in Brazil (Alves et al, 2018;Huber et al, 2007;Thomaz, Meireles, Soares, Pena, & Gennari, 2007).…”

Section: Resultssupporting

confidence: 88%

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Neto

Widmer

Lima

et al. 2020

Semina: Ciênc. Agrár.

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We investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders.

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Section: Resultssupporting

confidence: 88%

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Neto

Widmer

Lima

et al. 2020

Semina: Ciênc. Agrár.

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“…C. parvum is considered to be a zoonotic species, rarely found in dogs. [3,6,21,22]. C. felis, as one of the most frequently found species in cats, was also occasionally detected in humans, suggesting the possibility of transmission between humans and cats [4,7,23,24].…”

Section: Resultsmentioning

confidence: 98%

“…Cryptosporidium is a small (4-6µm), widespread, coccidian parasite affecting the epithelium of the digestive and respiratory tracts in mammals, including humans, reptiles, amphibians and birds [1,2]. Cryptosporidium genus contains over 30 so far recognized species and numerous genotypes [3]. Cats may be infected with C. felis, C. muris, C. ryanae and C. parvum and dogs with C. canis, C. parvum, C. ubiquitum and C. andersoni [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

<i>Cryptosporidium</i> spp. in dogs and cats in Poland

Piekara-Stępińska¹,

Piekarska²,

Gorczykowski³

2021

Ann Agric Environ Med.

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Introduction. Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats. Objective. The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland. Materials and method. A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene. Results. Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats. Conclusion. This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.

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“…and G. duodenalis are commonly detected in dogs and cats worldwide [4, 5] . Cryptosporidium canis and C. felis are major Cryptosporidium species in dogs and cats respectively, but C. hominis , C. parvum , C. muris and C. ubiquitum have been occasionally detected in these animals [6–10]. Similarly, dog-adapted assemblages C and D, and cat-adapted assemblage F are the dominant G. duodenalis genotypes in these animals, although zoonotic assemblages A and B have been identified in some studies [2, 11].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China

Dan

Zhu

et al. 2019

Parasites Vectors

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BackgroundThere are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats in Guangdong, China.MethodsPCR and sequence analyses were used to identify and genotype Cryptosporidium spp. and G. duodenalis in fecal samples from 641 dogs and 418 cats in Guangdong. Chi-square test and odds ratio analysis were used to compare the occurrence rates of these pathogens and identify risk factors for infection.ResultsThe overall infection rates of Cryptosporidium spp. and G. duodenalis were 6.9% (44/641) and 9.4% (60/641) in dogs, and 6.2% (26/418) and 3.6% (15/418) in cats. Purebred cats (12.4%; χ2 = 5.110, OR = 2.8, P = 0.024) and dogs (10.8%; χ2 = 5.597, OR = 4.8, P = 0.018) were more likely to be infected by Cryptosporidium spp. and G. duodenalis, respectively. Dogs (12.0%; χ2 = 7.589, OR = 2.6, P = 0.006) and cats (13.6%; χ2 = 8.235, OR = 3.5, P = 0.004) under 6 months had significantly higher infection rates of Cryptosporidium spp. than older animals. Household (13.9%; χ2 = 10.279, OR = 2.6, P = 0.008) and pet shop dogs (11.0%; χ2 = 7.182, OR = 2.0, P = 0.048) had higher occurrence of Cryptosporidium spp., as was the case for G. duodenalis occurrence in experimental dogs (13.4%; χ2 = 9.223, OR = 1.9, P = 0.017). Cryptosporidium canis (n = 42), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in dogs, while C. felis (n = 21), C. parvum (n = 3), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in cats. In contrast, the canine-specific assemblages C (n = 27) and D (n = 26) and the feline-specific assemblage F (n = 14) were almost exclusively the only genotypes of G. duodenalis in dogs and cats, respectively. There was no significant difference in infection rates of Cryptosporidium spp. and G. duodenalis between diarrheal and non-diarrheal pets.ConclusionsWhile domestic pets in Guangdong are infected with zoonotic Cryptosporidium species, they are mainly infected with host-specific G. duodenalis genotypes. Risk factors for infections differ between Cryptosporidium spp. and G. duodenalis and between dogs and cats.

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Molecular detection of Cryptosporidium spp. and the occurrence of intestinal parasites in fecal samples of naturally infected dogs and cats

Cited by 28 publications

References 39 publications

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

<i>Cryptosporidium</i> spp. in dogs and cats in Poland

Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China

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