Molecular detection of circulating cancer cells during surgery in patients with biliary-pancreatic cancer

Miyazono, Futoshi; Takao, Sonshin; Natsugoe, Shoji; Uchikura, Keiichiro; Kijima, Fumio; Aridome, Kuniaki; Shinchi, Hiroyuki; Aikou, Takashi

doi:10.1016/s0002-9610(99)00086-0

Cited by 61 publications

(29 citation statements)

References 16 publications

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“…There have been several studies on the detection of circulating cancer cells in the blood by PCR or RT-PCR. 10 20 detected CEA-mRNA in the circulating peripheral blood in patients with gastric cancer using their own CEA-specific RT-PCR method. Thus, the CEA-specific RT-PCR method is useful for detecting circulating cancer cells in patients with gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Surgical Maneuvers Enhance Molecular Detection of Circulating Tumor Cells During Gastric Cancer Surgery

Miyazono

Natsugoe²,

Takao³

et al. 2001

Annals of Surgery

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ObjectiveTo evaluate the relation between the presence of cancer cells in blood according to the time course during a surgical procedure and liver metastases in patients with gastric cancer. Summary Background DataSeveral studies have reported on the detection of circulating cancer cells in blood by reverse transcriptase-polymerase chain reaction (RT-PCR). However, few reports have examined the relation between molecular detection of circulating cancer cells according to the time course during a surgical procedure and blood-borne metastases. MethodsBlood samples from 57 patients with gastric cancer were obtained from the portal vein, peripheral artery, and superior vena cava before and after tumor dissection. After total RNA was extracted from each blood sample, carcinoembryonic antigen (CEA)-specific RT-PCR was performed. ResultsCEA-mRNA was detected in the blood of 21 (36.8%) of the 57 patients. CEA-mRNA was not detected in the blood obtained from 15 healthy volunteers and 15 patients with benign disease. The positive rate increased in proportion to the depth of tumor. The incidence of positive CEA-mRNA did not differ among the various sites of blood sampling. The appearance of circulating cancer cells was related to the surgical maneuver. A significant relation was found between the detection of CEA-mRNA and blood-borne metastases. ConclusionsA high incidence of positive CEA-mRNA was found in the blood during gastric cancer surgery. Surgical maneuvers are a possible cause of hematogenous metastasis. The authors found that patients with positive CEA-mRNA had a high risk of blood-borne metastasis even after curative resection.Blood-borne metastasis, including liver metastasis, is one of several important prognostic factors in patients with gastric cancer undergoing curative resection.

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Section: Discussionmentioning

confidence: 99%

Surgical Maneuvers Enhance Molecular Detection of Circulating Tumor Cells During Gastric Cancer Surgery

Miyazono

Natsugoe²,

Takao³

et al. 2001

Annals of Surgery

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“…(12,13) The RT-PCR is a useful assay for detecting circulating tumor cells (CTC) within blood specimens from patients with various malignancies, including gastric cancer. (14)(15)(16)(17)(18) We reported that the presence of CTC correlates with tumor progression and outcomes of patients with esophageal, gastric and biliary-pancreatic cancer.(14-18) Several epithelial-specific antigens such as CEA, cytokeratin-19, and cytokeratin-20, have been used generally in many studies of CTC detection in gastric cancer. (15,19) However, recent studies have found false-positive results in RT-PCR assays using these markers for CTC detection.…”

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B7‐H3 expression in gastric cancer: A novel molecular blood marker for detecting circulating tumor cells

et al. 2011

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The clinical significance of B7-H3 expression in gastric cancer remains unclear, although the B7 ligand family plays a critical role in the T cell-mediated immune response. We therefore investigated B7-H3 expression as a blood marker of circulating tumor cells and determined correlations with tumor progression in patients with gastric cancer. B7-H3 expression in gastric cell lines was initially evaluated by immunocytochemistry. Furthermore, we used quantitative RT-PCR to assess B7-H3 mRNA expression in four cell lines and in 95 blood specimens from patients with gastric cancer, as well as in 21 samples of peripheral blood lymphocytes from healthy volunteers. B7-H3 expression in cell lines was identified by immunocytochemistry and quantitative RT-PCR. Blood specimens from patients with gastric cancer contained significantly more copies of B7-H3 mRNA than those from healthy volunteers without cancer (P < 0.0001). Levels of B7-H3 expression significantly correlated with overall stage (P = 0.013). The 5-year survival rate was significantly lower in patients with high B7-H3 expression than with low expression (P = 0.02). Multivariate analysis demonstrated that B7-H3 expression was an independent prognostic factor (P = 0.046). Our results indicate that B7-H3 appears to be a useful blood marker for predicting tumor progression in gastric cancer. (Cancer Sci 2011; 102: 1019-1024 G astric cancer is one of the most common gastrointestinal tract malignancies in Asia.(1-4) New anticancer agents such as S-1, taxanes, capecitabine, irinotecan and oxaliplatin have improved the prognosis of patients with unresectable advanced gastric cancer.(5-9) Nevertheless, the 5-year survival rates of patients with International Union Against Cancer (UICC) stage IIIA, IIIB, and IV gastric cancers are 30.8-54.0%, 16.1-36.5% and 9.2-23.9%, respectively.(10,11) Carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) are commonly used as established tumor markers in blood for patients with gastric cancer. However, the sensitivity and specificity of predicting tumor progression and postoperative recurrence are clinically insufficient and no molecular biomarkers can yet predict the prognosis of patients with gastric cancer. (12,13) The RT-PCR is a useful assay for detecting circulating tumor cells (CTC) within blood specimens from patients with various malignancies, including gastric cancer. (14)(15)(16)(17)(18) We reported that the presence of CTC correlates with tumor progression and outcomes of patients with esophageal, gastric and biliary-pancreatic cancer.(14-18) Several epithelial-specific antigens such as CEA, cytokeratin-19, and cytokeratin-20, have been used generally in many studies of CTC detection in gastric cancer. (15,19) However, recent studies have found false-positive results in RT-PCR assays using these markers for CTC detection. (20,21) Consequently, RT-PCR assays using conventional CTC markers have low specificity for detecting occult CTC from blood specimens. Furthermore, few candidate molecular blood markers of...

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“…Ozawa (10) described the anterior transection technique in 1990. This technique was popularized over the next decade (11)(12)(13)(14)(15), driven by the perceived disadvantages of conventional right liver mobilization, namely impaired hepatic blood flow with rotational displacement of the liver (10), potential avulsion of hepatic veins (13), iatrogenic tumour rupture (13,16), and hematogenous dissemination of malignant cells when the hepatic veins remained patent (16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

Modified hanging manoeuvre facilitates inferior vena cava resection and reconstruction during extended right hepatectomy: A technical case report

Cawich

Thomas

Ragoonanan

et al. 2017

Molecular and Clinical Oncology

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Abstract. Liver resections are safe when performed by specialized hepatobiliary teams. However, complex liver resections are accompanied by significant perioperative risk and they may require modifications of the conventional surgical techniques. We herein report the case of a 54-year-old male patient who underwent an extended right liver resection with en bloc resection and reconstruction of the inferior vena cava. For this complex resection, a modification of the standard operative technique was required. A modified hanging manoeuvre was performed using two 19Fr nasogastric tubes outside the traditional avascular plane to facilitate resection. This modification of the hanging manoeuvre was proven to be feasible and safe, and it is recommended for inclusion in the armamentarium of hepatobiliary surgeons when complex resections are required.

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Molecular detection of circulating cancer cells during surgery in patients with biliary-pancreatic cancer

Cited by 61 publications

References 16 publications

Surgical Maneuvers Enhance Molecular Detection of Circulating Tumor Cells During Gastric Cancer Surgery

Surgical Maneuvers Enhance Molecular Detection of Circulating Tumor Cells During Gastric Cancer Surgery

B7‐H3 expression in gastric cancer: A novel molecular blood marker for detecting circulating tumor cells

Modified hanging manoeuvre facilitates inferior vena cava resection and reconstruction during extended right hepatectomy: A technical case report

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