Molecular detection of Brucella spp. from broth culture of clinical samples in Nigeria: Its role in vaccine quality control

Abstract

PCR was employed to detect Brucella spp. from broth cultures of clinical samples using a group specific primer based on IS6501 sequence. The sensitivity and specificity of this assay was confirmed by Southern hybridization analysis using a digoxigenin-labeled DNA probe while reproducibility of the analysis was confirmed by repetition of the test. Also, ERI 1 and ERI 2 primers were used to differentiate Brucella abortus strain 19 from other strains and the relevance in quality control of Brucella vaccine production highlighted.