Molecular Detection of Avipoxvirus in Wild Birds in Central Italy

Bertelloni, Fabrizio; Ceccherelli, Renato; Cossato, Margherita Marzoni Fecia Di; Poli, Alessandro; Ebani, Valentina Virginia

doi:10.3390/ani12030338

Cited by 3 publications

(2 citation statements)

References 34 publications

(46 reference statements)

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“…One cycle was performed at 72°C for 10 minutes after these cycles. The clade of the poxvirus-positive sample was determined by amplifying the locus fpv140 by PCR using the forward primer (5'-GAAGTAGAGTTATCGGTTC-3') and the reverse primer M2912 (5'-GGTGATCCATTTCCATTTC-3'), as described elsewhere [13,14]. The fpv140 PCR distinguishes between clades A and B, which are 1,800 and 2,400 base pairs long, respectively.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Khaleefah,

Al-Tameemi,

Kraidi

et al. 2024

Korean J Vet Res

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Bird species, particularly poultry and other bird types, including domestic pigeons, are susceptible to fowl pox, a contagious viral disease. The main goal of this study was to validate clinical avipoxvirus diagnoses using molecular analytical methods. The essential components of the investigation were the clinical signs, visible abnormalities, histological changes, and polymerase chain reaction analysis. Twenty out of 120 pigeons had clinical symptoms, which included yellowish crust or nodules near the feet, eyes, and beak. An erosive epidermal lesion and an epidermal acanthotic papular lesion with basal vacuolation were maculopapular evidence associated with significant epidermal hyperkeratosis, as confirmed by histological analysis. In addition, the results showed keratinocyte necrosis beneath the hyperkeratotic epidermal layer, together with superficial and deep dermal perivascular lymphocytic infiltration. In addition, the P4b core protein gene underwent phylogenetic analysis. The sequence analysis results indicated a high degree of similarity across the local strains, with just minor variations observed. Five sample sequences were selected and submitted to the NCBI database. These sequences were identified as OR187728, OR187729, OR187730, OR187731, and OR187732. All the various strains in this research may be classified under clade A of the chicken pox virus phylogenetic classification. This study presents the first description and characterization of pox virus infections in domestic pigeons inside the Basrah governorate.

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Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Khaleefah,

Al-Tameemi,

Kraidi

et al. 2024

Korean J Vet Res

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“…These are caused by different routes of infection. The cutaneous form is caused by mechanical trauma [ 17 ] and is responsible for nodular lesions in featherless areas, being associated with very low mortality rates [ 18 ]. The diphtheric form, on the other hand, occurs after inhalation or ingestion of the virus [ 17 ] and is characterized by the formation of proliferative nodular lesions in the mucous membranes of the digestive and respiratory tracts [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023

Santos,

Fagulha,

Duarte

et al. 2023

Veterinary Sciences

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Avipoxvirus (APV), a linear dsDNA virus belonging to the subfamily Chordopoxvirinae of the family Poxviridae, infects more than 278 species of domestic and wild birds. It is responsible for causing avian pox disease, characterized by its cutaneous and diphtheric forms. With a high transmission capacity, it can cause high economic losses and damage to the ecosystem. Several diagnostic methods are available, and bird vaccination can be an effective preventive measure. Ten APV-positive samples were analyzed to update the molecular characterization and phylogenetic analysis of viruses isolated in Portugal between 2017 and 2023. A P4b gene fragment was amplified using a PCR, and the nucleotide sequence of the amplicons was determined using Sanger sequencing. The sequences obtained were aligned using ClustalW, and a maximum likelihood phylogenetic tree was constructed. With this study, it was possible to verify that the analyzed sequences are distributed in subclades A1, A2, B1, and B3. Since some of them are quite similar to others from different countries and obtained in different years, it is possible to conclude that there have been several viral introductions in Portugal. Finally, it was possible to successfully update the data on Avipoxviruses in Portugal.

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Molecular and pathological screening of the current circulation of fowlpox and pigeon pox virus in backyard birds

Mohamed,

Elsamadony,

Alghamdi

et al. 2024

Poultry Science

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Molecular Detection of Avipoxvirus in Wild Birds in Central Italy

Cited by 3 publications

References 34 publications

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023

Molecular and pathological screening of the current circulation of fowlpox and pigeon pox virus in backyard birds

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