Molecular Detection of Antimicrobial Susceptibility: Changing Paradigm of Laboratory Testing for Multidrug-Resistant Organisms

Malone, Leslie; Stonebraker, Megan; Brzeziński, S.; Stalons, Donald; Hassoun, Ali; Brozka, Pius; Fantin, Nicole; Varma, Kamini; Trotta, Joshua; Grigorenko, Elena L.

doi:10.1093/ofid/ofv133.1149

Cited by 4 publications

(3 citation statements)

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“…This unscrupulous use of antibiotics has abetted the growing number of resistant bacteria in clinical settings. In the present study, half of the respondents would take antibiotics to relieve fever and pain, which was similar to a study whose participants thought antibiotics could relieve most cold symptoms [ 28 ]. Some participants believe antibiotics are less resistant with increasing duration of use.…”

Section: Discussionsupporting

confidence: 76%

Assessment of public knowledge and attitude toward antibiotics use and resistance: a community pharmacy-based survey

Isah,

Aina,

Ben-Umeh

et al. 2023

J of Pharm Policy and Pract

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Background Antimicrobial resistance is a public health challenge affecting all aspects of healthcare systems. Policies to reduce antimicrobial resistance should be implemented and monitored in community pharmacies, because they are patients' first point of care. Public awareness of relevant knowledge and attitudes on antimicrobials is a feasible strategy to mitigate the spread of antimicrobial resistance by exploiting the relationship between pharmacists and patients in the community pharmacy setting. The study evaluated and determined predictors of antibiotic knowledge and attitudes toward antibiotic use and resistance in community pharmacy patients. Methods A cross-sectional design was used to retrieve data in five randomly selected community pharmacies in Lagos and Abuja using a self-administered questionnaire. Descriptive and inferential statistics were utilized for characterizing and determining the associations between predictors and outcomes at p < 0.05. Logistic regression was used to identify predictors of patients’ knowledge and attitude to antibiotic use. Results A total of 964 clients participated in the study: 526(54.7%) were females, and 358(37.3%) were aged 25–34. chlorpheniramine–maleate and levonorgestrel were wrongly identified as antibiotics by 621 (64%) and 490 (50%) respondents, respectively. Many respondents, 448(46.5%), strongly agree that antibiotic creams should be mixed with body creams. The result of the multivariable logistic regression showed secondary education [Odds Ratio (OR): 0.31, 95% CI 0.10–0.97, p value: 0.044], urban residence (OR: 1.45, 95% CI 1.01–2.08, p value: 0.043) and age 34 (OR: 1.55, 95% CI 1.01–2.37, p value: 0.045) were strong predictors of knowledge on antibiotics, while community pharmacy location (OR: 5.48, 95% CI 3.45–8.70, p value: ≤ 0.001), urban residence (OR: 2.57, 95% CI 1.67–3.96, p value: ≤ 0.001), and antibiotic recommender (OR: 0.55, 95% CI 0.35–0.85, p value: 0.008) were predictors of respondents’ attitude to antibiotic use. Conclusions The study established that sociodemographic factors could impact community pharmacy clients' knowledge and attitude toward antibiotic use and resistance and should be considered when developing policies to curb the spread of resistant microbes. Community pharmacies should educate community pharmacy clients on the dangers associated with the misuse of antibiotics with stringent antibiotic stewardship programs and restrict access to antibiotics over-the-counter.

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Section: Discussionsupporting

confidence: 76%

Assessment of public knowledge and attitude toward antibiotics use and resistance: a community pharmacy-based survey

Isah,

Aina,

Ben-Umeh

et al. 2023

J of Pharm Policy and Pract

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“…They are rarely reported as a cause of clinical infection. Facklamia can be associated with invasive diseases such as urinary tract infections, chorioamnionitis, and infective endocarditis (Malone et al 2017;Rahmati et al 2017). The results also showed that Bacteroides abundance was not significantly altered by cephalosporin.…”

Section: Discussionmentioning

confidence: 87%

Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy

et al. 2020

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Abstract. Nurgaziyev M, Atenov Y, Khassenbekova Z, Akpanova S, Rysbekov K, Kozhakhmetov S, Nurgozhina A, Sergazy S, Chulenbayeva L, Ospanova Z, Tuyakova A, Mukhambetganov N, Sattybayeva R, Urazova S, Galymgozhina N, Zhumadilova A, Gulyaev A, Kushugulova A. 2020. Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy. Biodiversitas 21: 5065-5071. Antibiotic treatment can severely affect the gut microbiome for short-term and long-term consequences. Probiotic and prebiotic supplements are widely prescribed to modulate the composition and function of the human gut microbiome. The current study aims to determine the impacts of mare’s milk prebiotics on the diversity of gut bacterial communities and the local immune system when administered during and after a course of antibiotic therapy. Six children aged 4 to 5 years diagnosed with bilateral bronchopneumonia were prescribed cephalosporin (cefuroxime) antibiotics. During the 60 days of the study, three children consumed mare’s milk prebiotics, while the other three did not. Fecal samples were collected daily during antibiotic therapy and every five days after the last day of antibiotic treatment. Total DNA was isolated, and the taxonomic composition of the gut microbiome was analyzed by sequencing the 16S rRNA gene (V1-V3 region). The MULTIPLEX MAP platform was used to evaluate the local immune status. The relative abundance of 11 genera was reduced and did not recover until the last day of the study. The abundance of Bacteroides was not significantly altered in either group. Christensenella, Rothia, Abiotrophia, Acinetobacter, Anaerotruncus, Holdemania, and Turicibacter numbers were significantly increased on day five and remained at the same level during the study period. Cephalosporin administration also reduced the levels of pro-inflammatory and anti-inflammatory cytokines/chemokines (MIP1α, TNFα, GMCSF, GCSF, sCD40L, FGF2, TGFα, IL1α, and IP10).

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“…Procalcitonin has created abundant interest in the previous years as an acute phase protein in bacterial infections, and there are lot of evidence to support its usefulness in specific infections. [3] World Health Organization (WHO) declared Coronavirus disease 2019 (COVID-19) as a global pandemic in March 2020. The pandemic continues to be a public health concern even today.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Acute Phase Proteins in Post-SARS CoV2 Mucormycosis Infection

Dixit,

Shettar,

Joshi

et al. 2024

Ann of Pathol and Lab Med

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Background This study was aimed to estimate the serum levels of acute phase reactants/proteins (APR) which includes serum ferritin, C Reactive Protein, D-dimer and Procalcitonin in mucormycosis infection following SARS CoV2 infection and to determine the clinical, biochemical and histopathological findings in the same. Methods It was an observational cross-sectional study. All cases of mucormycosis following SARS CoV2 infection were reviewed. Demographic and clinical details with comorbidities and relevant laboratory investigations which included serum glucose, serum ferritin, C Reactive Protein, D-dimer, Procalcitonin were noted at the time of diagnosis of mucormycosis. Diagnosis of mucormycosis was based on frozen section examination and histopathological examination. Results Thirty-two cases of mucormycosis following SARS CoV2 infection confirmed on histopathology were studied. Majority of the cases (77%) were with comorbidity especially having diabetic mellitus type 2. C-Reactive protein, Procalcitonin and serum Ferritin were raised in almost all cases with mean of 80.92±62.5mg/l, 17.99±26.87ng/ml. and 464.9±422.03ng/ml respectively. D Dimer was raised in less than 50% cases with a mean of 828.5±245.9 ng/ml. Conclusion Acute phase proteins like C reactive Protein, Procalcitonin, Serum ferritin and D Dimer were raised in Post-SARS CoV2 infected individual who presented now with mucormycosis infection. These markers were raised markedly in critically ill patients thus indicated its pathogenetic role in severe morbidity, thus estimation of serum acute phase reactants can help in predicting the course and severity of illness.

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Molecular Detection of Antimicrobial Susceptibility: Changing Paradigm of Laboratory Testing for Multidrug-Resistant Organisms

Cited by 4 publications

References 1 publication

Assessment of public knowledge and attitude toward antibiotics use and resistance: a community pharmacy-based survey

Assessment of public knowledge and attitude toward antibiotics use and resistance: a community pharmacy-based survey

Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy

Distribution of Acute Phase Proteins in Post-SARS CoV2 Mucormycosis Infection

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