Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran

Momtaz, Hassan; Rahimi, Ebrahim; Moshkelani, S.

doi:10.17221/5916-vetmed

Cited by 89 publications

(76 citation statements)

References 19 publications

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“…Previous studies conducted in cattle disagree: some have reported that the tet(A) determinant is dominant in E. coli isolates recovered from cattle (23)(24)(25), whereas others found tet(B) to be dominant (26)(27)(28). In the present study, the prevalences of tet(A) and tet(B) were almost equal at 46.5% and 45.1%, respectively, which is consistent with other reports that showed a similar distribution pattern for the tet gene in E. coli isolates recovered from animals (23,29). The degree of resistance to tetracycline is associated with the presence of tet(B) (10).…”

Section: Discussionsupporting

confidence: 90%

Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle

Shin

Jung

et al. 2015

Appl Environ Microbiol

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bThe aim of this study was to investigate the prevalence and transferability of resistance in tetracycline-resistant Escherichia coli isolates recovered from beef cattle in South Korea. A total of 155 E. coli isolates were collected from feces in South Korea, and 146 were confirmed to be resistant to tetracycline. The tetracycline resistance gene tet(A) (46.5%) was the most prevalent, followed by tet(B) (45.1%) and tet(C) (5.8%). Strains carrying tet(A) plus tet(B) and tet(B) plus tet(C) were detected in two isolates each. In terms of phylogenetic grouping, 101 (65.2%) isolates were classified as phylogenetic group B1, followed in decreasing order by D (17.4%), A (14.2%), and B2 (3.2%). Ninety-one (62.3%) isolates were determined to be multidrug resistant by the disk diffusion method. MIC testing using the principal tetracyclines, namely, tetracycline, chlortetracycline, oxytetracycline, doxycycline, and minocycline, revealed that isolates carrying tet(B) had higher MIC values than isolates carrying tet(A). Conjugation assays showed that 121 (82.9%) isolates could transfer a tetracycline resistance gene to a recipient via the IncFIB replicon (65.1%). This study suggests that the high prevalence of tetracycline-resistant E. coli isolates in beef cattle is due to the transferability of tetracycline resistance genes between E. coli populations which have survived the selective pressure caused by the use of antimicrobial agents.A ntimicrobial resistance in humans and animals is considered a problem worldwide. Resistance to antimicrobial agents impedes the effective prevention and treatment of infectious disease, and thus, many governments have planned and implemented national programs for monitoring resistance in humans and animals (1-4). Surveillance data show that the inadequate selection and extensive use of antimicrobials result in the emergence and spread of resistant bacteria, particularly multidrug-resistant bacteria, and increase resistance to newer compounds, such as tetracycline-class antimicrobials (5).The tetracyclines are one of the most widely used classes of antimicrobial agents in human and veterinary medicine because they have several advantages, which include a broad spectrum of activity, low cost, oral administration, and few side effects (6). After chlortetracycline was introduced into clinical medicine in 1948, many derivatives, such as tetracycline, oxytetracycline, doxycycline, and minocycline, were developed, and today, these derivatives are widely used to treat disease and as growth promoters in the food animal industry. However, the widespread and indiscriminate use of tetracyclines has subjected bacterial populations to selection pressure and increased the prevalence of tetracycline resistance (6, 7).Tetracycline resistance is generally caused by the acquisition of a tetracycline resistance (tet) gene, as these genes are associated with primary resistance mechanisms, which involve active efflux pumps, ribosomal protection, and enzyme inactivation (8). To date, more than 40 different resist...

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Section: Discussionsupporting

confidence: 90%

Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle

Shin

Jung

et al. 2015

Appl Environ Microbiol

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“…The ndings from this study are inline previous studies regarding the frequency of bla TEM in members of Enterobacteriaceae family [40,41,42]. The isolates also exhibited frequent occurrence of other antibiotic resistant genes including aacA2, aadA, strA and strB which confer against aminoglycosides.…”

Section: Discussionsupporting

confidence: 88%

Occurrence and antibiogram signatures of members of the Enterobacteriaceae family recovered from vegetables, river water and hospital effluents in Amathole and Chris Hani District Municipalities in the Eastern Cape Province, South Africa.

Mpaka¹,

Adefisoye²,

Nontongana³

et al. 2020

Preprint

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Background Rising incidences of antimicrobial resistance has become a major public health issue globally resulting in increasing health-care costs and severe and lethal diseases. Several reports have documented alarming increases of antimicrobial resistance in the Enterobacteriaceae family, hence, this study evaluated the occurrence and antibiogram signatures of Enterobacteriaceae isolates recovered from vegetables, hospital effluents and river water samples in two district municipalities in the Eastern Cape Province, South Africa. Results Out of 142 presumptive isolates, 105 were confirmed to belong to the Enterobacteriaceae family. From these, 45% were identified as E. coli, 24% as Enterobacter spp., 20% as Citrobacter spp. and 11% as Klebsiella spp. All the isolates demonstrated high resistance against ampicillin at a frequency of 91.4%, followed by nalidixic acid (86.7%), tetracycline (82.9%), cefuroxime (81.9%) and doxycycline (81.9%). The beta-lactam resistance gene blaTEM was detected in 77.8% of the E. coli isolates and 33.3% of the Klebsiella isolates. The sul1 gene was detected in 23.1% of the Enterobacter species, while sul2 gene was detected in 70% of the Klebsiella species. All the Enterobacter species were positive for the strB gene. Conclusions We conclude that the vegetables, hospital effluents and river water in the two District Municipalities are reservoirs of multidrug resistant members of the Enterobacteriaceae family and a potential health hazard for consumers.

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“…; Momtaz et al . ). The chosen gene tetW is chromosomally encoded and common in Gram positive bacteria (Scott et al .…”

Section: Discussionmentioning

confidence: 97%

“…those grazing with and those grazing without cattle, quantification by culture independent qPCR was performed for one common tetracycline resistance gene and one sulphonamide resistance gene. qPCR has previously been used in several investigations to quantify levels of resistance genes (Pei et al 2006;Momtaz et al 2012). The chosen gene tetW is chromosomally encoded and common in Gram positive bacteria (Scott et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in faecal samples from buffalo, wildebeest and zebra grazing together with and without cattle in Tanzania

et al. 2015

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Molecular detection of antimicrobial resistance genes in E. coli isolated from slaughtered commercial chickens in Iran

Cited by 89 publications

References 19 publications

Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle

Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle

Occurrence and antibiogram signatures of members of the Enterobacteriaceae family recovered from vegetables, river water and hospital effluents in Amathole and Chris Hani District Municipalities in the Eastern Cape Province, South Africa.

Antimicrobial resistance in faecal samples from buffalo, wildebeest and zebra grazing together with and without cattle in Tanzania

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