Molecular detection of Anaplasma platys, Ehrlichia canis, Hepatozoon canis and Rickettsia monacensis in dogs from Maio Island of Cape Verde archipelago

Lauzi, Stefania; Maia, João P.; Epis, Sara; Marcos, Ricardo; Pereira, Cristina Camargo; Luzzago, C.; Santos, Marta; Puente-Payo, Pablo; Giordano, Alessia; Pajoro, Massimo; Sironi, G.; Faustino, Augusto

doi:10.1016/j.ttbdis.2016.05.001

Cited by 38 publications

(34 citation statements)

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“…According to previous surveys, there was not an association with gender (Rojas et al 2014;Lauzi et al 2016;Aktas et al, 2017;Licari et al 2017), although male dogs had a slightly increased, but not significant, risk of contracting H. canis infection in the present study. Due to their predilection for roaming behavior, male dogs may have higher environmental exposure to TBDs.…”

Section: Discussioncontrasting

confidence: 61%

Hepatozoon canis in hunting dogs from Southern Italy: distribution and risk factors

et al. 2020

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Hepatozoon canis is a hemoprotozoan organism that infects domestic and wild carnivores throughout much of Europe. The parasite is mainly transmitted through the ingestion of infected ticks containing mature oocysts. The aims of the present survey were to determine the prevalence of H. canis in hunting dogs living in Southern Italy and to assess potential infection risk factors. DNA extracted from whole blood samples, collected from 1433 apparently healthy dogs living in the Napoli, Avellino, and Salerno provinces of Campania region (Southern Italy), was tested by a quantitative real-time polymerase chain reaction (qPCR) assay to amplify H. canis. Furthermore, the investigated dog population was also screened by qPCR for the presence of Ehrlichia canis, a major tick-borne pathogen in Southern Italy, in order to assess possible co-infections. Two hundred dogs were H. canis PCR-positive, resulting in an overall prevalence of 14.0% (CI 12.2-15.9). Breed category (P < 0.0001), hair coat length (P = 0.015), and province of residence (P < 0.0001) represented significant risk factors for H. canis infection. The presence of H. canis DNA was also significantly associated with E. canis PCR positivity (P < 0.0001). Hunting dogs in Campania region (Southern Italy) are frequently exposed to H. canis, and the infection is potentially associated with close contact with wildlife. Further studies are needed to assess the pathogenic potential of H. canis, as well as the epidemiological relationships between hunting dogs and wild animal populations sharing the same habitats in Southern Italy.

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Section: Discussioncontrasting

confidence: 61%

Hepatozoon canis in hunting dogs from Southern Italy: distribution and risk factors

et al. 2020

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“…One common factor that has been observed in different studies is coinfection even in animals without clinical evidence of infection (LAUZI et al 2016). In the present study, such animals accounted for 13.4% (37/276) of the sample.…”

Section: Agent ------------------------------------Group-------------supporting

confidence: 57%

“…the clinical signs of these infections are not specific, laboratory support is required in order to establish a confirmatory diagnosis. Studies have shown that molecular diagnostic techniques have high sensitivity and specificity for these infections (LAUZI et al, 2016;AZHAHIANAMBI et al, 2018;VLAHAKIS et al, 2018).…”

Section: Parasitologymentioning

confidence: 99%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

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The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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“…While E. canis is generally believed to cause more severe febrile condition in dogs, E. ewingii infection is less severe (Liddell et al, 2003). Signs of the disease in dogs may include but are not limited to fever, anaemia, haemorrhages, oedema, lymphadenopathy, thrombocytopenia, anorexia and weight loss (Cicuttin et al, 2016;Lauzi et al, 2016). The organism is characterised by the presence of intracytoplasmic inclusion bodies (morulae) in the monocytes/granulocytes of infected dogs, which can be detected by microscopic examination of peripheral blood (Dagnone et al, 2009).…”

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confidence: 99%

Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

Daramola

Takeet

Oyewusi

et al. 2018

Acta Veterinaria Hungarica

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Canine ehrlichiosis is an important tick-borne rickettsial disease mainly caused by Ehrlichia canis. This study aimed to detect and characterise E. canis in dogs in Abeokuta, Nigeria by microscopy and nested PCR. Blood samples were collected from 205 dogs, thin smears were made, field-stained, and DNA was extracted from the blood samples. A partial region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced unidirectionally. Ehrlichial morulae were detected in three dogs (1.5%). The PCR test revealed that 47 dogs (22.9%) were positive for E. canis. The lengths of the sequences obtained range from 374 bp to 376 bp with an average G-C content of 37% and 98-99% homology with the reference sequences in GenBank. The aligned autochthonous sequences were less polymorphic. The phylogenetic analysis separated sequences reported previously in Nigeria from the autochthonous sequences. The present work shows that the strain of E. canis detected in the study area is genetically different from those reported in the northern part of Nigeria and more closely related to sequences from Brazil and India.

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Molecular detection of Anaplasma platys, Ehrlichia canis, Hepatozoon canis and Rickettsia monacensis in dogs from Maio Island of Cape Verde archipelago

Cited by 38 publications

References 50 publications

Hepatozoon canis in hunting dogs from Southern Italy: distribution and risk factors

Hepatozoon canis in hunting dogs from Southern Italy: distribution and risk factors

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

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