Molecular Detection of Acarapis woodi Using Hive Debris as Innovative and Non-Invasive Matrix

Pietropaoli, Marco; Tofani, Silvia; Formato, Giovanni; Rubino, Roberta C.; Pietrella, Gabriele; Ruggiero, Camilla Di; Milito, Marcella; Merola, Carmine; Amorena, Michele; Cersini, Antonella

doi:10.3390/app12062837

Cited by 5 publications

(3 citation statements)

References 11 publications

(24 reference statements)

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“…Other methods based on DNA analysis have been recently developed to detect A. woodi in bees (Garrido-Bailón et al, 2012;Pietropaoli et al, 2022). These methods for the observation of the interior of the tracheae of the bees are more sensitive and less laborious than those involving the dissection of the specimens.…”

Section: Resultsmentioning

confidence: 99%

Acarapis woodi, endoparasite of honeybees, from being qualified as the pest of the century to a forgotten case in Mexico

Otero Colina,

Hernández-Dionicio,

Utrera-Quintana

et al. 2023

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Objetivo: Este trabajo se realizó para determinar la incidencia de Acarapis woodi (Rennie) infestando las tráqueas de abejas melíferas (Apis mellifera L.) en colonias no tratadas previamente contra el ácaro Varroa destructor Anderson & Trueman, y para discutir el estatus actual del primer ácaro en México, con base en nuestras observaciones y en información histórica. Diseño/metodología/aproximación: Colonias de abejas melíferas (Apis mellifera L.) que nunca habían sido tratadas contra el ácaro V. destructor fueron muestreadas para detectar a A. woodi en el interior de sus tráqueas torácicas. Se tomó una muestra de 20 obreras de cada colonia, se les conservó en etanol 70% y se les examinó máximo dos días deepués de la colecta. Se seleccionaron obreras pecoreadoras que se encontraban en los panales laterales, la entrada de la colmena y la tapa interior porque ahí se encuentran las abejas más viejas, con más probabilidad de estar infestadas. Para el diagnóstico se usó la técnica de diescción y observación al microscopio. Resultados: Ninguna de las colonias examinadas, ninguna de las abejas, presentaron infestación en las tráqueas. Limitaciones del estudio/implicaciones: Aunque solo se observe un apiario, éste mostró la tendencia general observada en México y en muchos otros países en América, sobre todo en sitios tropicales y subtropicales. Después de una onda expansiva de A. woodi, las infestaciones por este ácaro se volvieron menos y menos frecuentes hasta que son muy difíciles de detectar. Hallazgos/conclusiones: La prevalencia cada vez más reducida de A. woodi ha sido atribuida a preferencias climáticas y a tratamientos contra V. destructor. Sin embargo, aquí se postula que esta reducción se debe al desarrollo de resistencia por parte de las abejas melíferas.

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Section: Resultsmentioning

confidence: 99%

Acarapis woodi, endoparasite of honeybees, from being qualified as the pest of the century to a forgotten case in Mexico

Otero Colina,

Hernández-Dionicio,

Utrera-Quintana

et al. 2023

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“…Recently, techniques based on ampli cation and sequencing of the COI gene have been frequently used for the identi cation of A. woodi. In this context, molecular techniques give more sensitive and speci c results, especially in case of low parasite loads (Garrido-Bailón et al 2012; Pietropaoli et al 2022).…”

Section: Introductionmentioning

confidence: 99%

PCR-based detection of the honeybee tracheal mite (Acarapis woodi) in Türkiye

Akpınar

Sevim

et al. 2023

Parasitol Res

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Acarapis woodi (Rennie, 1921) (Acari: Tarsonemidae) is one of the mites that settles in the respiratory system of honeybees (Apis mellifera L. (Hymenoptera, Apidae)) and distributed throughout the world. It causes signi cant economic losses on honey production. In Türkiye, studies on the existence of A. woodi are very limited and so far, no studies on the molecular diagnosis and phylogenetic of it have been reported in Türkiye. This study was conducted to investigate the prevalence of A. woodi in Türkiye, especially in areas where beekeeping is intense. Diagnosis of A. woodi was performed using both microscopic and molecular methods using speci c PCR primers. Adult honeybee samples were collected from 1.193 hives in 40 provinces of Türkiye between 2018 and 2019. Based on identi cation studies, the presence of A. woodi was detected in a total of 3 hives (0.5%) in 2018 and 4 hives (0.7%) in 2019. This is the rst report for determination of A. woodi in Türkiye.

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“…The viruses BQCV, SBV, and CBPV, followed by V. ceranae and L. passim, were the most prevalent pathogens, and many of the investigated hives, despite being asymptomatic, had different degrees of co-infection. By means of an end-point PCR, which was confirmed by Sanger sequencing, Pietropaoli et al [20] detected the presence of A. woodi in samples of honeybees and hive debris collected from apiaries in the Latium region (Central Italy). A potential underestimation of the occurrence of A. woodi in Italian apiaries is hypothesized.…”

mentioning

confidence: 96%

Special Issue: “Frontier Research in Apiculture (Diagnosis and Control of Bee Diseases, Bee Products, Environmental Monitoring)”

Mutinelli

2023

Applied Sciences

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Molecular Detection of Acarapis woodi Using Hive Debris as Innovative and Non-Invasive Matrix

Cited by 5 publications

References 11 publications

Acarapis woodi, endoparasite of honeybees, from being qualified as the pest of the century to a forgotten case in Mexico

Acarapis woodi, endoparasite of honeybees, from being qualified as the pest of the century to a forgotten case in Mexico

PCR-based detection of the honeybee tracheal mite (Acarapis woodi) in Türkiye

Special Issue: “Frontier Research in Apiculture (Diagnosis and Control of Bee Diseases, Bee Products, Environmental Monitoring)”

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