Molecular detection of a polymorphism in the p53 codon 72 gene and its association with prostate cancer

Abstract

The role of the current study in detecting polymorphisms in the gene P53codon72, a gene that has morphological polymorphism and has an important role in the incidence of diseases, especially cancers such as prostate cancer, samples for the study were collected from 50 patients with prostate cancer from the auditors of the Imam Hussein Center for Cancer and Diseases Blood in Al-Hussein, peace be upon him, Teaching Hospital - Holy Karbala Governorate - Iraq After diagnosis by a specialist surgeon and 50 phenotypically healthy individuals as a control group, the (DNA) was extracted from the blood of both healthy and all patients study groups, and molecular detection of polymorphism of nucleotides was performed in Genes of the study using PCR-RFLP Molecular detection results in P53codon72 The results showed that there were highly significant differences between the genotype (Pro-Pro) and between the infected and healthy groups as well as the genotype (Arg-Arg), while there was no significant relationship between the genotype (Arg-Pro) and between the infected and healthy groups. The results also showed highly significant differences between the three genotypes (Arg/ Arg, Aro/Pro, Pro/Pro) and between the two groups sick and healthy.