Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae)

Abstract

ABSTRACT. Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and MRS (de Man, Rogosa, and Sharpe) agar, and then randomly select colonies from each plate and stocked them to the mother plate for genomic DNA isolation. Second, we analyzed bacterial colonies using the 16S rRNA gene molecular diagnostic method. Based on bacterial cultures and bacterial 16S rRNA gene markers, we detected four different bacterial species (Bacillus amyloliquefaciens, Escherichia coli, Staphylococcus xylosus, and Serratia liquefaciens) from fecal coliforms of the striped field mouse Apodemus agrarius and A. peninsulae in agricultural areas in South Korea. These results could help us to better understand the pathogen reservoirs of mice and initiate some preventive measures to mitigate the microbial risks associated with mouse fecal matter in agricultural production areas.

Predicting the zoonotic capacity of mammals to transmit SARS-CoV-2

“…Also in the top 10% were two rodent species considered to be human commensals whose geographic ranges are expanding due to human activities: Rattus argentiventer (0.84) and R. tiomanicus (0.79) (Supplementary File 1) [119][120][121]. Additional common rodent species with relatively high probabilities of zoonotic capacity include domesticated guinea pigs (Cavia porcellus), gerbils (Gerbillus gerbillus, Meriones tristrami), and several common mouse species (Apodemus peninsulae, A. flavicollis, and A. sylvaticus), all of which are known reservoirs for other zoonotic diseases [122][123][124]. It is notable that many of these rodent species are regularly preyed upon by carnivore species, such as the red fox (Vulpes vulpes) or domestic cats (Felis catus) who themselves were predicted to have high zoonotic capacity for SARS-CoV-2 by our model.…”

Molecular characterization of antimicrobial resistance genes on farms and in commercial milk with emphasis on the effect of currently practiced heat treatments on viable but nonculturable formation

“…PCR Probes and Protocol for Identification of Bacteria. A previously published adapted PCR protocol using 16S rRNA species-specific probes for screening of E. coli, staphylococci, and streptococci were used (Table 1; Malhotra-Kumar et al, 2005;Lange et al, 2015;Rahman et al, 2017). The PCR amplification was conducted following a previously adopted protocol (Taher et al, 2020).…”

Prevalence of Salmonella spp. and Escherichia coli in the feces of free-roaming wildlife throughout South Korea

“…E . coli , STEC, and other bacterial species have been detected in fecal samples using a modified culture method [ 15 , 61 , 62 ]. First, fecal samples were cultured in a final volume of 10 mL (diluted 1:10) with non-selective buffered peptone water (BPW) overnight at 37°C.…”