Molecular Detection and Serological Evidence of Tick-Borne Encephalitis Virus in Serbia

Potkonjak, Aleksandar; Petrović, Tamaš; Ristanović, Elizabeta; Lalić, Ivica; Vračar, Vuk; Savić, Sara; Turkulov, Vesna; Canak, G; Milosevic, Vesna; Vidanović, Dejan; Jurišić, Aleksandar; Petrović, Aleksandra; Petrović, Vladimir

doi:10.1089/vbz.2017.2167

Cited by 28 publications

(28 citation statements)

References 49 publications

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“…Different seroprevalences of TBEV in dogs were reported in European countries: Belgium 0.1% (Roelandt et al, 2011), Czech Republic 3.3% (Klimeš et al, 2001), Denmark 4.8% (Lindhe et al, 2009), Finland 6.7% (Levanov et al, 2016), Germany 2.1% (Balling et al, 2015), Norway 16.4% (Csángó et al, 2004), and Spain 1.7-1.8% (Garcia-Bocanegra et al, 2018). In our previous investigation in Serbia, we identified anti-TBEV IgG antibodies in the sera of 7 dogs (17.5% of the dogs studied) in Vojvodina (Potkonjak et al, 2017). The main vector in Europe is I. ricinus, although Dermacentor reticulatus is important for the transmission of pathogens to dogs (Leschnik, 2020;Chitimia-Dobler et al, 2019).…”

Section: Tick-borne Encephalitis Virusmentioning

confidence: 72%

“…So far, we know Serbia is an endemic area for a large number of tick-borne infections (Potkonjak et al, 2016a). Most of the research has been related to proving the presence of pathogens in ticks (Potkonjak et al, 2017;Potkonjak et al, 2016a;Potkonjak et al, 2016b;Tomanović et al, 2013;Radulović et al, 2011;Savić et al, 2010;Tomanović et al, 2010a;Tomanović et al, 2010b;Milutinović et al, 2008), using molecular biology methods or cultivation in the case of Borrelia species (Čakić et al, 2019). Several research studies on tick-borne pathogens in domestic and wild animals have been published in recent years (Juwaid et al, 2019;Sukara et al, 2018;Vasić et al, 2018).…”

Section: O N L I N E F I R S Tmentioning

confidence: 99%

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Tick-borne infections of dogs in Serbia: A review of research

Potkonjak

Zekic-Stosic

2020

VET GLASNIK

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Increased prevalence of tick-borne disease in humans and animals is an important public health issue. Dogs can be clinically ill, reservoirs of tick-borne pathogens, or sentinel populations for research. Certainly, small-scale research has been conducted in dogs and this review will focus on the recent studies of emergent and re-emergent tick-borne pathogens in dogs in Serbia. Babesiosis stands out as the most common and important tick-borne disease in dogs. In addition to this pathogen, the presence of Hepatozoon canis and ?Candidatus Mycoplasma haematoparvum? in the blood of dogs was proved using molecular methods. Seroreactivity in dogs has been proven for Borrelia burgdorferi sensu lato (s.l.) complex, Ehrlichia canis, Ehrlichia ewingii, Anaplasma phagocytophilum, Anaplasma platys, Rickettsia conorii, and tick-borne encephalitis virus (TBEV). The presence of B. burgdorferi s.l. complex, A. phagocytophilum, Rickettsia spp. from the group of spotted fevers, and TBEV in ticks indicates the geographical distribution and potential for infecting humans, dogs, and other animals in Serbia. Further systematic, comprehensive, well designed, bacteriological, virological, parasitological, epidemiological, clinical (infectological), and acarological research is needed in Serbia.

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Section: Tick-borne Encephalitis Virusmentioning

confidence: 72%

Section: O N L I N E F I R S Tmentioning

confidence: 99%

Tick-borne infections of dogs in Serbia: A review of research

Potkonjak

Zekic-Stosic

2020

VET GLASNIK

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“…Ixodes ricinus is considered the most important vector of many zoonotic pathogens of viral and bacterial origin in Europe (Furness and Furness 2018). In Croatia, tick-borne encephalitis virus (TBEV) was isolated from adult I. ricinus ticks (Jemeršić et al 2014), while in neighboring Serbia, tickborne encephalitis virus (TBEV) was detected in nymphs (Potkonjak et al 2017). In earlier comprehensive faunistical studies of tick fauna in Croatia, I. ricinus was recorded on 24 hosts (Mikačić 1949, 1965, Tovornik 1987, 1990, Tovornik and Brelih 1980.…”

Section: Discussionmentioning

confidence: 99%

Diversity, ecology, and seasonality of hard ticks (Acari: Ixodidae) in eastern Croatia

Krčmar

2019

Journal of Vector Ecology

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The diversity of hard tick fauna was studied in different forest communities in 48 localities of eastern Croatia using the dragging‐flagging method and by hand from ten different vertebrate hosts. A total of 2,225 specimens comprising seven species in three genera were identified. Ixodes ricinus, the most abundant species (72.8% of all collected specimens) was recorded in 44 localities, followed by Dermacentor reticulatus in 19 localities, while the other five species (D. marginatus, H. concinna, H. inermis, I. canisuga, and I. hexagonus) were recorded in fewer localities. The highest ratio (67% of collected ticks) was in the adult stage with the predominance of females. The numbers of collected females, males, and nymphs of I. ricinus and H. concinna differed significantly. Ten new tick‐host associations in Croatia were recorded. Fifty‐three animals were infested with one tick species. Single species infestation with I. ricinus was found in 45.8% of animals, followed by D. reticulatus with 25.4%, D. marginatus with 10.2%, H. inermis and I. hexagonus with 3.4%, and I. canisuga with 1.7%. Coinfestation with two species of ticks were recorded in six animals. Ixodes ricinus, H. inermis, and D. reticulatus showed bimodal seasonal activity; for other species unimodal activity patterns were recorded.

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“…ELISA analysis of the aforementioned sera revealed the presence of TBEV-specific antibodies in 17.5% (7/40) of dogs, 5% (1/20) horses, 12.5% (5/40) wild boars, 2.5% (1/40) cattle and 2.5% (1/40) of roe deer. The presence of TBEV-specific antibodies was not established in any (0/20) of the examined goat blood sera(Potkonjak et al, 2017). Apart from the abovementioned research, TBEV was first established in animals in Serbia in 2017.…”

mentioning

confidence: 91%

“…One of the detected TBEV isolates was sequenced and phylogenetically typed as European (Western) TBEV subtype (Figure 5). In addition, low prevalence of TBEV antibodies was established by ELISA in 0.37% (1/267) of the examined blood sera collected in the same period from patients at the Clinic for Infectious Diseases of the Clinical Centre of Vojvodina(Potkonjak et al, 2017).Some recent data on the presence of TBEV in animals came from serological research that included 200 animal blood sera collected during 2014 and 2015. ELISA analysis of the aforementioned sera revealed the presence of TBEV-specific antibodies in 17.5% (7/40) of dogs, 5% (1/20) horses, 12.5% (5/40) wild boars, 2.5% (1/40) cattle and 2.5% (1/40) of roe deer.…”

mentioning

confidence: 99%

Flaviviruses at the territory of Serbia – present situation and challenges

Šekler¹,

Petrić²,

Vidanović³

et al. 2019

AVM

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The aim of this study is to summarize the data on the occurrence, presence and prevalence of some zoonotic flaviviruses, which have been actively circulating in the territory of the Republic of Serbia during past decade. The genus Flavivirus, family Flaviviridae,encompasses vector-transmitted viruses with zoonotic potential. The genus is comprised of more than 70 viruses transmitted to humans by mosquitoes and ticks. Some of those are associated only with human diseases (such as yellow fever and dengue fever), whereas others can cause diseases in both humans and animals. Animals are major reservoirs and primary hosts for the latter group. The virus transmission to other animal species or humans occurs via diverse vectors – mostly mosquitoes and ticks. A range of these virus species is widely distributed worldwide, especially in tropical and sub-tropical climatic zones. Some representatives of these species have only recently been identified at the territory of Republic of Serbia. This paper gives an evidence on the presence and distribution of West-Nile virus, tick-borne encephalitis (TBE) virus and Usutu virus that were conducted within the study in Serbia. The research pointed out the presence of recently detected West-Nile virus and Usutu virus as well as the current circulation of tick-borne encephalitis virus, for which only limited serological evidence on the presence of virus-specific antibodies in humans was available so far. In the aspect of public health, the full understanding of the importance of identification and prevalence of different flaviviruses still require further comprehensive entomologicalacarological, seroepidemiological, clinical and virological research.

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Molecular Detection and Serological Evidence of Tick-Borne Encephalitis Virus in Serbia

Cited by 28 publications

References 49 publications

Tick-borne infections of dogs in Serbia: A review of research

Tick-borne infections of dogs in Serbia: A review of research

Diversity, ecology, and seasonality of hard ticks (Acari: Ixodidae) in eastern Croatia

Flaviviruses at the territory of Serbia – present situation and challenges

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