Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil

Cândido, Marcelo; Alencar, Anna Luiza Farias; Almeida-Queiroz, Sabrina Ribeiro de; Buzinaro, Maria da Glória; Munin, Flávia Simone; Godoy, Silvia Helena Seraphin de; Livonesi, Márcia Cristina; Fernandes, Andrezza Maria; Sousa, Ricardo Luiz Moro de

doi:10.1007/s00705-015-2400-8

Cited by 16 publications

(23 citation statements)

References 19 publications

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“…To clarify this possibility, a screening of different rodent species would be necessary. Bovine astroviruses are distributed worldwide (De Benedictis et al., ; Tse et al., ; Oem and An, ; Alfred et al., ; Candido et al., ), and two other bovine cases of astrovirus–encephalitis were described, which argues for the link between astroviruses and encephalitis, which is also suggested here. A potential contamination, as it is documented for metagenomics approaches (Naccache et al., ; Rosseel et al., ), is most likely excluded, because the control animals were negative in RT‐qPCR screening as with FISH.…”

Section: Resultssupporting

confidence: 72%

Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis

Schlottau

Schulze²,

Bilk³

et al. 2016

Transbound Emerg Dis

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Encephalitis can be caused by several infectious agents, including bacteria, fungi, parasites and viruses. In many cases, the causative agent cannot be identified, because the pathogens are unknown or detection methods are not routinely available. In our case, a 15-month-old cow developed central nervous disorders and died within 6 days after the onset of clinical signs. The histopathology revealed an acute encephalitis, predominantly in the brain stem, and a ganglionitis of the trigeminal ganglion with massive neuronal necroses in both the brain and the ganglion. However, a relevant panel of bacterial and viral infections of cattle could be routinely excluded. Therefore, a brain sample from the cow was analysed using a metagenomics approach with next-generation sequencing. A novel bovine astrovirus (BoAstV-BH89/14) could be identified using the analysis pipeline RIEMS, and the finding could be confirmed with a specific BoAstV RT-qPCR. The genome of the bovine astrovirus (BoAstV), belonging to the family Astroviridae in the genus Mamastrovirus, has a length of 6478 bp. Sequence identities between 71% to a sheep astrovirus and 69% to two recently described bovine astroviruses from the USA and Switzerland were ascertained. The latter were also connected to encephalitis cases in cattle. Like these, the new virus described here was detected in different brain sections using the specific BoAstV RT-qPCR and fluorescent in situ hybridization. In conclusion, while astroviruses so far were mainly found in relation to gastroenteritis in animals and humans, recently detected astrovirus infections were also related to encephalitis.

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Section: Resultssupporting

confidence: 72%

Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis

Schlottau

Schulze²,

Bilk³

et al. 2016

Transbound Emerg Dis

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“…We reported BAstV in feces of calves with clinical diarrhea (32 %), which clustered with BAstV lineage 1 [27]. Lineage 1 involves one and nine strains of genogroup G2 and G3, respectively [28], in addition to five Korean strains within genogroup G1 [26]. The Egyptian BAstV strains were closely related to isolates from Brazil and Japan.…”

Section: Discussionmentioning

confidence: 90%

Molecular detection of enteric viruses from diarrheic calves in Egypt

et al. 2016

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Neonatal calf diarrhea (NCD) is a major cause of morbidity, mortality and economic losses in the beef and dairy industries. This study was conducted to investigate the existence of enteric viruses in two Egyptian farms with a history of recurrent diarrhea. Fecal samples were collected from 25 diarrheic calves. RNA was extracted and tested by reverse transcription polymerase chain reaction (RT-PCR) for the presence of rotavirus, norovirus, astrovirus, torovirus, coronavirus and bovine viral diarrhea virus. Overall, 76 % (19/25) of samples tested positive for one or more viruses. Rota-, noro- and astroviruses were detected in 48 %, 24 % and 32 % of tested samples, respectively. About 37 % (7/19) of positive samples had two different viruses. One-month-old calves were the group most vulnerable to infections. Based on phylogenetic analysis, bovine rotaviruses were of genotypes G6 and G10, bovine noroviruses were in GIII.2, and bovine astroviruses were in the BAstV lineage 1. Astrovirus sequences showed a high level nucleotide sequence similarity with the Brazilian BAstV sequences available in GenBank. We believe this is the first report of bovine norovirus and bovine astrovirus circulating among calves in Egypt. Further epidemiological studies are recommended to investigate their presence on a wider scale, to predict their association with NCD, and to design appropriate diagnostic and control methods.

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“…Almost all BoAstVs clustered with BoAstV lineage 1, including Chinese BoAstVs, while three Japanese strains and one American strain clustered with lineage 2. Recently, Korean and Brazilian BoAstVs were phylogenetically analyzed [21,22]. These studies employed partial genome sequences corresponding to the ORF1b and/or ORF2 genome regions.…”

Section: Discussionmentioning

confidence: 99%

Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus

et al. 2015

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A viral metagenomics approach was used to investigate fecal samples of Japanese calves with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly complete astrovirus-related RNA sequences in 15 of the 146 fecal samples collected in three distinct areas (Hokkaido, Ishikawa, and Kagoshima Prefectures) between 2009 and 2015. Due to the lack of genetic information about bovine astroviruses (BoAstVs) in Japan, these sequences were analyzed in this study. Nine of the 15 Japanese BoAstVs were closely related to Chinese BoAstVs and clustered into a lineage (tentatively named lineage 1) in all phylogenetic trees. Three of 15 strains were phylogenetically separate from lineage 1, showing low sequence identities, and clustered instead with an American strain isolated from cattle with respiratory disease (tentatively named lineage 2). Interestingly, two of 15 strains clustered with lineage 1 in the open reading frame (ORF)1a and ORF1b regions, while they clustered with lineage 2 in the ORF2 region. Remarkably, one of 15 strains exhibited low amino acid sequence similarity to other BoAstVs and was clustered separately with porcine astrovirus type 5 in all trees, and ovine astrovirus in the ORF2 region, suggesting past interspecies transmission.

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Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil

Cited by 16 publications

References 19 publications

Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis

Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis

Molecular detection of enteric viruses from diarrheic calves in Egypt

Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus

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