Molecular detection and identification of enteroviruses in children admitted to a university hospital in Greece

Siafakas, Nikolaos; Attilakos, Achilleas; Vourli, S.; Stefos, Efstathios; Meletiadis, Joseph; Nikolaidou, Paraskevi; Zerva, Loukia

doi:10.1016/j.mcp.2011.06.001

Cited by 18 publications

(10 citation statements)

References 44 publications

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“…According to previous reports, CV-A2 could exist nearly year-round, and the CV-A2 phylogeny suggests the wide geographic circulation of distinct genotypes [7, 22, 23]. This indicates that systematic knowledge of CV-A2 is essential.…”

Section: Discussionmentioning

confidence: 97%

Two Genotypes of Coxsackievirus A2 Associated with Hand, Foot, and Mouth Disease Circulating in China since 2008

Yang¹,

Zhang²,

Yan³

et al. 2016

PLoS ONE

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Coxsackievirus A2 (CV-A2) has been frequently detected and commonly associated with hand, foot, and mouth disease (HFMD) in China since 2008. However, limited sequences of CV-A2 are currently available. As a result, we have been focusing on the genetic characteristics of CV-A2 in the mainland of China during 2008–2015 based on national HFMD surveillance. In this study, 20 CV-A2 strains were isolated and phylogenetic analyses of the VP1 sequences were performed. Full-length genome sequences of two representative CV-A2 isolates were acquired and similarity plot and bootscanning analyses were performed. The phylogenetic dendrogram indicated that all CV-A2 strains could be divided into four genotypes (Genotypes A–D). The CV-A2 prototype strain (Fleetwood) was the sole member of genotype A. From 2008 to 2015, the CV-A2 strains isolated in China dispersed into two different genotypes (B and D). And the genotype D became the dominant circulating strains in China. Strains isolated in Russia and India from 2005 to 2011 converged into genotype C. Intertypic recombination occurred between the Chinese CV-A2 strains and other enterovirus-A donor sequences. This result reconfirmed that recombination is a common phenomenon among enteroviruses. This study helps expand the numbers of whole virus genome sequence and entire VP1 sequence of CV-A2 in the GenBank database for further researcher.

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Section: Discussionmentioning

confidence: 97%

Two Genotypes of Coxsackievirus A2 Associated with Hand, Foot, and Mouth Disease Circulating in China since 2008

Yang¹,

Zhang²,

Yan³

et al. 2016

PLoS ONE

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“…In Iran, only EV71 of subgenotype C1 was detected from a child with AFP in 2005 (90). In Greece, all EV71 strains detected from children with HFMD, febrile illness, or maculopapular rash belonged to subgenotype C2 in 2009–2010 (109). Coexistence of more than one subgenotype was observed in some other regions (Table 3).…”

Section: Methodsmentioning

confidence: 99%

Human enterovirus 71 epidemics: what's next?

Yip

Lau

Woo

et al. 2013

Emerging Health Threats Journal

148

163

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Human enterovirus 71 (EV71) epidemics have affected various countries in the past 40 years. EV71 commonly causes hand, foot and mouth disease (HFMD) in children, but can result in neurological and cardiorespiratory complications in severe cases. Genotypic changes of EV71 have been observed in different places over time, with the emergence of novel genotypes or subgenotypes giving rise to serious outbreaks. Since the late 1990s, intra- and inter-typic recombination events in EV71 have been increasingly reported in the Asia-Pacific region. In particular, ‘double-recombinant’ EV71 strains belonging to a novel genotype D have been predominant in mainland China and Hong Kong over the last decade, though co-circulating with a minority of other EV71 subgenotypes and coxsackie A viruses. Continuous surveillance and genome studies are important to detect potential novel mutants or recombinants in the near future. Rapid and sensitive molecular detection of EV71 is of paramount importance in anticipating and combating EV71 outbreaks.

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“…Human enteroviruses (EVs) belong to the family Picornaviridae and are among the most common causes of acute viral illness in childhood [11]. A recent study described the identification of strains belonging to human EV species B and A in children and the detection of the emerging pathogen EV71 for the first time in Greece [12]. To evaluate the epidemiological and clinical role of different viruses in sporadic pediatric gastroenteritis patients in Italy, a 1-year study (2008-2009) showed that RV was the pathogen most frequently detected (38.3%), with AdVs detected at 2.6% and EVs not detected at all [1].…”

Section: Figmentioning

confidence: 99%

“…A recent investigation of EV circulation in children identified strains of EV-B and EV-A. All reported EV-A serotypes of that study, were detected for the first time in Greece, and - most notably- six of them were identified as EV71 [12]. …”

Section: Figmentioning

confidence: 99%

Rare Types of Rotaviruses Isolated from Children with Acute Gastroenteritis in Patras, Greece

et al. 2013

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Background: Acute diarrhea remains a major public health issue worldwide, with gastroenteritis agents associated with a high morbidity, especially in infants and young children. A 1-year study (2009-2010) of rotavirus (RV)-caused pediatric diarrhea was performed on hospitalized children admitted with symptoms of acute diarrhea to the University Hospital of Patras, Greece. Methods: Twenty-nine fecal samples were investigated for RVs, adenoviruses (AdVs), and enteroviruses (EVs) in an attempt to characterize these enteric viruses, which have been implicated in hospitalized diarrhea. Results: A 44.8% (13/29) incidence of viral infection was reported for the viral targets. Monoinfections accounted for 31% (9/29), while bi-infections accounted for 13.4% (4/29). Sequencing of positive samples allowed identification of RVs G4P[8] (2/29), G9P[8] (1/29), and interestingly of the rare type G12P[8] (2/29). AdV types 1, 2, and 6 were also identified in 4/29, 1/29, and 1/29 of the samples, respectively. Regarding the EVs, EV71 (2/29), coxsackievirus A4 (1/29), echovirus 11 (1/29), and EV96 (1/29) were typed. Conclusion: The results of the present study, and specifically the detection of rare RV G12 and EV71 strains, address the need for continuous epidemiological surveys to provide epidemiological pictures of pediatric viral infections circulating in the community.

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Molecular detection and identification of enteroviruses in children admitted to a university hospital in Greece

Cited by 18 publications

References 44 publications

Two Genotypes of Coxsackievirus A2 Associated with Hand, Foot, and Mouth Disease Circulating in China since 2008

Two Genotypes of Coxsackievirus A2 Associated with Hand, Foot, and Mouth Disease Circulating in China since 2008

Human enterovirus 71 epidemics: what's next?

Rare Types of Rotaviruses Isolated from Children with Acute Gastroenteritis in Patras, Greece

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