Molecular detection and identification of tick-borne bacteria and protozoans in goats and wild Siberian roe deer (Capreolus pygargus) from Heilongjiang Province, northeastern China

Wang, Haoning; Yang, Jifei; Mukhtar, Muhammad Uzair; Liu, Zhijie; Zhang, Minghai; Wang, Xiaolong

doi:10.1186/s13071-019-3553-1

Cited by 24 publications

(17 citation statements)

References 31 publications

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“…In China, Babesia motasi, B. ovis (9,10), Theileria ovis, T. uilenbergi, T. luwenshuni (4)(5)(6), Anaplasma phagocytophilum, A. marginale, A. ovis, A. bovis, A. capra, and A. platys (11,12) are the causative agents of ovine tick-borne disease transmitted by ticks or small ruminant that have been reported.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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Babesia, Theileria, and Anaplasma are important causative agents of tick-borne diseases that severely affect sheep. However, there is paucity in the occurrence genetic diversity of the infections of tick-borne diseases in sheep in border regions, northwestern China. In this study, nested polymerase chain reaction (nPCR) assays and gene sequencing were used to identify tick-borne Babesia spp., Theileria spp., and Anaplasma spp. infections in border regions, northwestern China. Out of 323 samples tested in this study, 225 (69.7%) sheep were infected with Babesia spp., Theileria spp., and Anaplasma spp. Two hundred six (63.8%), 60 (18.6%), 54 (16.7%), 51 (15.8%), 32 (9.9%), 19 (5.9%), and 16 (5.0%) were positive for A. ovis, B. motasi-like, A. bovis, T. uilenbergi, A. phagocytophilum, T. luwenshuni, and B. motasi-like Xinjiang, respectively. The most common dual infection was with A. ovis and B. motasi-like while the most frequent triple coinfection was A. ovis, B. motasi-like, and T. uilenbergi with coinfection rates of 17.0% (55/323) and 5.0% (16/323), respectively. Sequencing analysis indicated that A. ovis MSP4, A. phagocytophilum epank1, A. bovis 16S rRNA, B. motasi-like rap1-b, B. motasi-like Xinjiang rap1-a, T. luwenshuni 18S rRNA, and T. uilenbergi 18S rRNA from border regions, northwestern China, showed 99-100% identity with documented isolates from other countries. To the best of the authors' knowledge, this is the first report of T. uilenbergi and T. luwenshuni infections of sheep in border regions, northwestern China. Furthermore, these findings provide important data for understanding the distribution of Babesia, Theileria, and Anaplasma in sheep between border countries and China.

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Section: Introductionmentioning

confidence: 99%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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“…cDNA was synthesized using GoScript Reverse Transcription System and 5'-UTR of BDV was ampli ed according to Access RT-PCR System (Promega, USA) (Liu et al, 2019). The DNA samples were detected for the presence of the genus Anaplasma by PCRbased ampli cation of the 16S rRNA gene for A. bovis, A. phagocytophilum, A. centrale and A. platys, the msp4 gene for A. ovis and A. marginale, and the citrate synthase (gltA) gene and 16S rRNA gene for A. capra, respectively, as previously described (Li et al, 2015;Torina et al, 2012;Wang et al, 2019;Yang et al, 2016). For piroplasm (Theileria spp.…”

Section: Nucleic Acid Extraction and Pcr Ampli Cationmentioning

confidence: 99%

“…and Babesia spp.) detection, all samples were screened using nested PCR assays targeting the 18S rRNA gene (Wang et al, 2019). Other vector-borne bacteria including Rickettsia spp.…”

Section: Nucleic Acid Extraction and Pcr Ampli Cationmentioning

confidence: 99%

Vector-Borne Pathogens With Veterinary and Public Health Significance in Melophagus Ovinus (Sheep Ked) From the Qinghai-Tibet Plateau

Zhang

Wang

Liu

et al. 2020

Preprint

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BackgroundMelophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmission cycle of pathogenic organisms. MethodsHerein, we investigated the presence and molecular characterization of vector-borne pathogens in M. ovinus from Qinghai-Tibet Plateau, China. ResultsA total of 92 M. ovinus pools (n=276) collected from Qinghai province of China were screened for the presence of selected vector-borne pathogens. The overall prevalence of A. ovis, A. bovis, A. phagocytophilum, and T. ovis in M. ovinus was 39.1%, 17.4%, 9.8%, and 89.1%, respectively. All of the samples were negative for BDV, other Anaplasma species, Babesia spp., Rickettsia spp., and Borrelia spp. Co-infection of different Anaplasma species and T. ovis occurred in 51.2% of all samples with T. ovis. The positive rates of A. ovis, A. bovis, and A. phagocytophilum in different region and altitude of the sampling sites were significantly different. Sequence and phylogenetic analysis of target genes confirmed their identity with corresponding pathogens. ConclusionOur results elucidate the occurrence and genetic diversity of Anaplasma spp. and Theileria spp. in M. ovinus, which could act as potential zoonotic reservoirs. To the best of our knowledge, this is the first report of the detection of A. bovis and A. phagocytophilum DNA in M. ovinus. This study gives the first extensive molecular survey of vector-borne pathogens with veterinary and public health significance in M. ovinus from the Qinghai-Tibet Plateau, China.

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“…Over the last few decades, members of the genus Anaplasma have been found in unexpected hosts, for example, in several domestic livestock species (sheep, cattle, and goats), and wild ruminants ( Snorre et al, 2013 ). Some of have also been found to occur in humans, such as Anaplasma phagocytophilum ( Dumler et al, 2005 ) , A.ovis ( Chochlakis et al, 2010 ) , and A. capra ( Hao et al, 2015 ) have been reported in roe deer ( Capreolus pygargus ), red deer ( Cervus elaphus ) and sika deer in China ( Li et al, 2016 ; Wang et al, 2019 ). A. bovis was found as the causative agent for anaplasmosis, detected in red deer and roe deer in southern Norway ( Razanske et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, few studies have investigated Anaplasma spp. infections in deer in China ( Wang et al, 2019 ; Yang et al, 2017b ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Anaplasma spp. in Tian Shan wapiti deer (Cervus elaphus songaricus) in Xinjiang, China

Cui

Xiao

et al. 2021

International Journal for Parasitology: Parasites and Wildlife

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Anaplasma spp. are important zoonotic tick-borne pathogens that impact on human health. There are few reports on the prevalence and molecular genetic characteristics of Cervidae species in China. The purpose of this study, therefore, was to investigate the presence of Anaplasma spp. in blood samples of Tian Shan wapiti ( Cervus elaphus songaricus ) in the Xinjiang Uygur Autonomous Region of China, and conduct phylogenetic analyses. A total of 50 blood samples (wild deer n = 26, and captive deer n = 24) were collected from the deer. PCR was used to detect Anaplasma spp. in the blood samples. Forty percent (20) of the samples were found to contain Anaplasma spp. Three Anaplasma species DNA were detected in deer blood samples: A. bovis (n = 13), A. ovis (n = 18), and A. phagocytophilum (n = 11). Among the 20 Anaplasma spp. positive samples, 14 were mixed infection of two or three pathogens. The prevalence of Anaplasma species in wild deer was significantly higher than that of captive deer, 73.1% (19) vs 4.2% (1) respectively, ( p < 0.01). Two A. ovis sequence types (AB1, and AB2), three A. ovis sequence types (AO1-AO3), and one A. phagocytophilum sequence type (AP1) were obtained in this study. The sequences of AO1 shared 100% identity with a human isolate from Cyprus. Our results suggest that wild deer are more likely to become infected with Anaplasma spp. than captive individuals, and thus, could potentially transmit pathogens to humans.

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Molecular detection and identification of tick-borne bacteria and protozoans in goats and wild Siberian roe deer (Capreolus pygargus) from Heilongjiang Province, northeastern China

Cited by 24 publications

References 31 publications

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Vector-Borne Pathogens With Veterinary and Public Health Significance in Melophagus Ovinus (Sheep Ked) From the Qinghai-Tibet Plateau

Identification of Anaplasma spp. in Tian Shan wapiti deer (Cervus elaphus songaricus) in Xinjiang, China

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