Molecular detection and identification of piroplasms in sika deer (Cervus nippon) from Jilin Province, China

Abstract

BackgroundPiroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera Theileria and Babesia. Wildlife such as sika deer play an important role as reservoir hosts for several species of Theileria and Babesia. Using blood samples collected from sika deer, we investigated the epidemiology of Theileria spp. and Babesia spp. in sika deer from Jilin Province in China and identified those species that cause pathogenic infections in sika deer.MethodsSixty-eight blood samples of sika deer were collected from three areas of the Jilin Province in Northeast China. Genomic DNA was extracted, and the V4 hypervariable region of the 18S rRNA of the piroplasms was amplified using the nested PCR method. The selected positive samples were sequenced to identify species of Babesia and Theileria.ResultsPCR detection revealed that 24 samples were positive for Theileria and Babesia spp. (35.29 %, 95 % CI = 11.8-46.8). After alignment, a sequenced fragment for Theileria cervi was found to be the most prevalent from the obtained samples (22.06 %, 95 % CI = 11.8-49.6). Six sika deer samples were identified as being infected with a Theileria sp. that was similar to a Theileria sp. found from spotted deer in India. In addition to the results above, for the first time, we identified T. annulata infection from one sample of sika deer and Babesia sp. from two samples, which showed high identity with Babesia motasi found in sheep from China.ConclusionThe present study offers new data on the pathogens of piroplasmosis in sika deer in northeastern China. For the first time, sika deer was confirmed as a reservoir host for the T. annulata of cattle and the B. motasi of sheep, which was found in China.

Molecular detection of Theileria species and Babesia caballi from horses in Nigeria

“…A hemi-nested PCR (the reverse primer being used in each reaction) assay was used to amplify the hypervariable region of the 18S rRNA gene as previously described (Liu et al 2016 ). Briefly, the external PCR reaction was set up in a 19-μl total volume containing 10 μl Sigma 2× Jumpstart Red Taq mix, 7 μl of nuclease-free water, 1 μl of 10 μM RLB-F2, and RLB-R2 primers (Table 2 ) and the processed DNA disc from the FTA card.…”

Molecular survey of piroplasm species from selected areas of China and Pakistan

“…Escherichia coli Trans 5α (TaKaRa, China) was transformed and plasmid DNA from the selected clones was identified using PCR with the set of primers T7 (5'-TAA TAC GAC TCA CTA TAG GG-3') and SP6 (5'-ATT TAG GTG ACA CTA TAG-3') to verify the presence of correct inserts in selected clones before proceeding with the sequencing process. The reaction cycling in the second PCR was optimized with primer annealing at 54 °C for 30 s. The correct inserted clones were shipped to Genescript® (Shanghai, China) for sequencing [ 26 ].…”

“…The reaction cycling in the second PCR was optimized with primer annealing at 54 °C for 30 s. The correct inserted clones were shipped to Genescript® (Shanghai, China) for sequencing [ 26 ].…”

Erratum to: Molecular detection and identification of piroplasms in sika deer (Cervus nippon) from Jilin Province, China

“…Unfortunately, the original version of this article [ 1 ] contained an error. Within the results section, and in Fig.…”