Molecular detection and identification of Leishmania DNA and blood meal analysis in Phlebotomus (Larroussius) species

Remadi, Latifa; Chargui, Najla; Jiménez, Maribel; Molina, Ricardo; Haouas, Najoua; González, Estela; Chaabane-Banaouas, Raja; Salah, Eya Ben; Haddaji, Mohsen; Chaabouni, Yassine; Babba, Hamouda

doi:10.1371/journal.pntd.0008077

Cited by 28 publications

(21 citation statements)

References 71 publications

(100 reference statements)

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“…Our results showed that P. perniciosus was highly susceptible for all three L. (S.) tarentolae strains studied. Although several studies have reported the broad host range of P. perniciosus [54][55][56], its willingness to feed on geckos has not been recorded yet. Leishmania promastigotes isolated from P. perniciosus, even in females collected in Mediterranean sites where Se.…”

Section: Discussionmentioning

confidence: 99%

Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species

et al. 2021

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Leishmania (Sauroleishmania) tarentolae is transmitted by reptile-biting sand flies of the genus Sergentomyia, but the role of Phlebotomus sand flies in circulation of this parasite is unknown. Here, we compared the development of L. (S.) tarentolae strains in three Phlebotomus species: P. papatasi, P. sergenti, and P. perniciosus. Laboratory-bred sand flies were membrane-fed on blood with parasite suspension and dissected on days 1 and 7 post blood meal. Parasites were measured on Giemsa-stained gut smears and five morphological forms were distinguished. In all parasite-vector combinations, promastigotes were found in Malpighian tubules, often in high numbers, which suggests that this tissue is a typical location for L. (S.) tarentolae development in sand flies. All three studied strains colonized the hindgut, but also migrated anteriorly to both parts of the midgut and colonized the stomodeal valve. Significant differences were demonstrated between sand fly species: highest infection rates, high parasite loads, and the most frequent anterior migration with colonization of the stomodeal valve were found in P. perniciosus, while all these parameters were lowest in P. sergenti. In conclusion, the peripylarian type of development was demonstrated for three L. (S.) tarentolae strains in three Phlebotomus sand flies. We suggest paying more attention to Phlebotomus species, particularly P. perniciosus and P. papatasi, as potential secondary vectors of Sauroleishmania.

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Section: Discussionmentioning

confidence: 99%

Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species

et al. 2021

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“…The identification of bloodmeals from phlebotomines using partial sequences from PCR products of the mtDNA cyt b gene is suitable and widely used for this purpose ( Jiménez et al 2013 , Carvalho et al 2017 , Pereira Junior et al 2019 , Doe et al 2020 , Leão et al 2020 , Remadi et al 2020 , Lozano-Sardaneta et al 2021 , Rodrigues et al 2021 , Torchitte et al 2020 ). Six vertebrate species were found to be blood feeding sources of Ny.…”

Section: Discussionmentioning

confidence: 99%

Blood Feeding Sources of Nyssomyia antunesi (Diptera: Psychodidae): A Suspected Vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon

Pimentel

Uzcátegui

Lima

et al. 2022

Journal of Medical Entomology

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Present work aimed to identify blood feeding sources and attempt to detect Leishmania DNA in Nyssomyia antunesi, suspected vector of Leishmania sp., from a park in the urban center of Belém, the capital of Pará State, in the Brazilian Amazon. Entire bodies and gut contents of Ny. antunesi engorged females, previously captured in the urban park with Centers for Disease Control (CDC) light traps and aspiration on tree bases, were subjected to Leishmania and vertebrate DNA detection through amplification of the Leishmania mini-exon and vertebrate cytochrome b (cyt b) gene regions, respectively. The quality of DNA extraction from entire bodies was ensured through amplification of the dipteran cyt b region. The vertebrate cyt b amplicons were sequenced and compared with those available on GenBank. A maximum likelihood phylogenetic tree was constructed to assess the clustering patterns of these sequences. Leishmania DNA was not detected. The sequences of 13 vertebrate cyt b amplicons were considered informative, exhibiting similarity and clustering with the following six vertebrate species: Dasyprocta leporina (1), Cuniculus paca (1), Tamandua tetradactyla (4), Choloepus didactylus (4), Pteroglossus aracari aracari (2), Homo sapiens (1). The samples of D. leporina and C. paca were obtained from the CDC canopy, whereas the others were by aspiration from tree bases. The present results revealed the eclectic and opportunist blood-feeding behavior of Ny. antunesi, with birds and mammals, these last ones acting as potential reservoirs for Leishmania species, distributed throughout the vertical forest strata.

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“…This was the case of the emerging VL foci located in the arid bioclimatic zone of Central Tunisia that evidenced a high mobilization of water resources recent years [ 12 , 14 ]. In these emerging foci of high VL incidence, several entomological surveys have been carried out showing L. infantum infection of several Laroussius species, notably P. perniciosus , P. perfiliewi , and P. longicuspis [ 15 , 16 , 17 ], but also showed L. infantum infection of Phlebotomus papatasi [ 15 , 17 ] and Sergentomyia minuta [ 15 ]. These studies have also highlighted the role of P. perfiliewi in these particular foci [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In these emerging foci of high VL incidence, several entomological surveys have been carried out showing L. infantum infection of several Laroussius species, notably P. perniciosus , P. perfiliewi , and P. longicuspis [ 15 , 16 , 17 ], but also showed L. infantum infection of Phlebotomus papatasi [ 15 , 17 ] and Sergentomyia minuta [ 15 ]. These studies have also highlighted the role of P. perfiliewi in these particular foci [ 15 , 16 ]. To our knowledge, no information is available on L. infantum infection rate of phlebotomine sandflies in Northern VL endemic areas of low incidence where P. perniciosus and P. perfiliewi are known to be sympatric species [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity, Abundance and Leishmania infantum Infection Rate of Phlebotomine Sandflies in an Area with Low Incidence of Visceral Leishmaniasis in Northern Tunisia

et al. 2022

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We report the study of sandfly Leishmania infection in an area of low incidence of visceral leishmaniasis in Tunisia. Sandflies were collected monthly using CDC light-traps set in houses and animal shelters during May–November 2016 and 2017. All males were identified at the species level. A sample of 878 females including all gravid specimens was subjected to kDNA qPCR for Leishmania detection and parasite load estimation. Leishmania species were determined by ITS1 PCR sequencing, and species identification of infected sandflies was performed by DNA barcoding. Phlebotomus perfiliewi and P. perniciosus were the dominant species during the two-year period. However, comparison of their relative abundances showed that P. perniciosus was more abundant during peaks of 2017 with longer activity duration. Real-time kDNA PCR did not detect Leishmania infection in 2016, although it identified four positive specimens (0.7%) in 2017. All four infected specimens were identified as P. perniciosus. ITS1 PCR sequencing allowed L. infantum identification in one kDNA qPCR-positive specimen. This was a P. perniciosus gravid female with a high parasite load caught during the long-lasting peak of 2017. This work highlights the usefulness of multi-seasonal studies of sandfly dynamics and kDNA qPCR in screening Leishmania infection and determining L. infantum vectors in hypo-endemic foci of human leishmaniasis.

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Molecular detection and identification of Leishmania DNA and blood meal analysis in Phlebotomus (Larroussius) species

Cited by 28 publications

References 71 publications

Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species

Development of Various Leishmania (Sauroleishmania) tarentolae Strains in Three Phlebotomus Species

Blood Feeding Sources of Nyssomyia antunesi (Diptera: Psychodidae): A Suspected Vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon

Diversity, Abundance and Leishmania infantum Infection Rate of Phlebotomine Sandflies in an Area with Low Incidence of Visceral Leishmaniasis in Northern Tunisia

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