2016
DOI: 10.3923/aje.2017.37.44
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Molecular Detection and Genotyping of Toxoplasma gondii from Egyptian Isolates

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Cited by 30 publications
(21 citation statements)
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“…The overall viable T. gondii isolation rate of 38.5% in the current study shows a lower percentage compared to the report of (Berhanu, 2015) who reported 67.6%. However, this study indicates a higher percentage value than the report of (Elfadaly et al, 2017) (Opsteegh et al, 2011) 62.8% from the USA. The differences between these reports may be due to the density of T. gondii in tissues of sheep and goats, the type of tissue sampled and the strain or genotype of T. gondii.…”
Section: Bioassay In Micecontrasting
confidence: 95%
See 1 more Smart Citation
“…The overall viable T. gondii isolation rate of 38.5% in the current study shows a lower percentage compared to the report of (Berhanu, 2015) who reported 67.6%. However, this study indicates a higher percentage value than the report of (Elfadaly et al, 2017) (Opsteegh et al, 2011) 62.8% from the USA. The differences between these reports may be due to the density of T. gondii in tissues of sheep and goats, the type of tissue sampled and the strain or genotype of T. gondii.…”
Section: Bioassay In Micecontrasting
confidence: 95%
“…This study in cattle on the isolation rate of viable T. gondii (21.43%) via bioassay in mice is comparable with the report of Opsteegh et al (2016) who demonstrated 3.3% of viable T. gondii from selected European countries. However, the nding of the current study is higher than the reported isolation rate of 0% from Ethiopia in cattle (Berhanu, 2015), 0% from Egypt in cow (Elfadaly et al, 2017) through bioassay in mice and lower than the reported isolation rate of 100% (18/18) in experimentally infected cattle con rmed via bioassay in mice (Deksne & Kirjušina, 2013). The differences between this report and aforesaid reports may be due to the density of T. gondii in tissues of cattle, the type of tissue sampled, the digestion method used and the strain or genotype of T. gondii (Dubey, 2010).…”
Section: Bioassay In Micecontrasting
confidence: 77%
“…The strain used in the present study to induce chronic toxoplasmosis was the Tehran strain of T. gondii (type II) which kindly gained from Prof. Hossein Keshavarz (Tehran University of Medical Sciences, Tehran, Iran). The parasite was prepared by intraperitoneal inoculation of tissue cysts of T. gondii (15-20 cysts) from brain tissue of infected BALB/c mice every 3 months into new mice based on the technique explained by Elfadaly et al [19]. The mice model of latent toxoplasmosis was established according to the process reported by Mahmoudvand et al [20].…”
Section: Inducement Of Chronic Toxoplasmosis In Micementioning
confidence: 99%
“…Since one of the most important mechanisms of control of toxoplasmosis is the immune system, mainly cellular immunity, we evaluated the mRNA levels of some innate immunity mediators such as IFN-γ and IL12 by quantitative real time PCR [ 33 ]. The results demonstrated that although the mRNA levels of IFN-γ and IL-12 were elevated in all mice of experimental groups, a significant increase was observed in mice treated by MCEO at the doses of Ex2 and Ex3 of MCEO when compared with control groups.…”
Section: Discussionmentioning
confidence: 99%