Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia

Vlahakis, Pipina A; Chitanga, Simbarashe; Simuunza, Martin; Simulundu, Edgar; Qiu, Yongjin; Changula, Katendi; Chambaro, Herman M.; Kajihara, Masahiro; Takada, Ayato; Mweene, Aaron S.

doi:10.1016/j.ttbdis.2017.10.010

Cited by 22 publications

(32 citation statements)

References 32 publications

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“…The nine A. platys 16S rRNA gene sequences obtained in this study from two dogs (D25 and D33) (Apla1/16S) were conserved and identical to the A. platys 16S rRNA gene sequences described from dogs in Zambia (LC269820, LC269821 and LC269822) [22] and to the corresponding 1383 nt of the 16S rRNA gene from the recently published genome of A. platys strain S3 [51]. Apla1/16S differed by 5 nt from the A. platys type strain 16S rRNA gene sequence (M82801) published in 1992 [52].…”

Section: S Rrnasupporting

confidence: 61%

“…The Adog2/16S sequences obtained in this study were identical to the Anaplasma sp. ZAM dog sequences previously described in Zambia (LC269823) [22], while the Adog1/16S sequences differed by a single deletion. Adog1/16S and Adog2/16S differed by 4 nt from the Anaplasma sp.…”

Section: S Rrnamentioning

confidence: 95%

“…Four genes (16S rRNA, gltA, msp4 and ankA) known to be useful for phylogenetic analysis of A. phagocytophilum [22,[39][40][41][42] were amplified and sequenced from 32 qPCR-positive samples (cattle = 4; dogs = 11; AFI patients = 4; rodents = 8; ticks = 5 pools; Table S1). Amplicons could not be obtained from the remaining qPCR-positive samples.…”

Section: Characterization Of a Phagocytophilum By Multilocus Gene Sementioning

confidence: 99%

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Anaplasma phagocytophilum and Other Anaplasma spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa

et al. 2020

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DNA samples from 74 patients with non-malarial acute febrile illness (AFI), 282 rodents, 100 cattle, 56 dogs and 160 Rhipicephalus sanguineus ticks were screened for the presence of Anaplasma phagocytophilum DNA using a quantitative PCR (qPCR) assay targeting the msp2 gene. The test detected both A. phagocytophilum and Anaplasma sp. SA/ZAM dog DNA. Microbiome sequencing confirmed the presence of low levels of A. phagocytophilum DNA in the blood of rodents, dogs and cattle, while high levels of A. platys and Anaplasma sp. SA/ZAM dog were detected in dogs. Directed sequencing of the 16S rRNA and gltA genes in selected samples revealed the presence of A. phagocytophilum DNA in humans, dogs and rodents and highlighted its importance as a possible contributing cause of AFI in South Africa. A number of recently described Anaplasma species and A. platys were also detected in the study. Phylogenetic analyses grouped Anaplasma sp. SA/ZAM dog into a distinct clade, with sufficient divergence from other Anaplasma species to warrant classification as a separate species. Until appropriate type-material can be deposited and the species is formally described, we will refer to this novel organism as Anaplasma sp. SA dog.

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Section: S Rrnasupporting

confidence: 61%

Section: S Rrnamentioning

confidence: 95%

Section: Characterization Of a Phagocytophilum By Multilocus Gene Sementioning

confidence: 99%

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Anaplasma phagocytophilum and Other Anaplasma spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa

et al. 2020

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“…However, they did find that 65% of wild dogs and 13% of domestic dogs were infected with Hepatozoon. Recently, Vlahakis et al (2017) described the first molecular evidence of A. platys in domestic dogs in Lusaka.…”

Section: Introductionmentioning

confidence: 99%

Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia

Qiu

Kaneko

Kajihara

et al. 2018

Ticks and Tick-borne Diseases

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“…the clinical signs of these infections are not specific, laboratory support is required in order to establish a confirmatory diagnosis. Studies have shown that molecular diagnostic techniques have high sensitivity and specificity for these infections (LAUZI et al, 2016;AZHAHIANAMBI et al, 2018;VLAHAKIS et al, 2018).…”

Section: Parasitologymentioning

confidence: 99%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

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The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia

Cited by 22 publications

References 32 publications

Anaplasma phagocytophilum and Other Anaplasma spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa

Anaplasma phagocytophilum and Other Anaplasma spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa

Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

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