Molecular detection and characterization of potentially new Babesia and Theileria species/variants in wild felids from Kenya

Githaka, Naftaly; Konnai, Satoru; Kariuki, Edward; Kanduma, Esther; Shiro, Motoo; Ohashi, Kazuhiko

doi:10.1016/j.actatropica.2012.06.013

Cited by 16 publications

(20 citation statements)

References 37 publications

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“…Many of these genotypes have been identified in multiple antelope species, making designation according to host specificity difficult. One genotype from this clade has been identified in cheetah and shows a close relationship to a genotype found in giraffes (Githaka et al., 2012). …”

Section: Specificity Of Molecular Assays and The Species Conceptmentioning

confidence: 75%

A review of Theileria diagnostics and epidemiology

Mans

Pienaar

Latif

2015

International Journal for Parasitology: Parasites and Wildlife

215

214

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HighlightsSerological and molecular assays exist for most economic important Theileria species.Molecular assays are constantly being improved with regard to sensitivity and specificity.The concept of what constitute a Theileria species impacts on accurate diagnostics.Analytical specificity of molecular assays are >800 000 parasites/L blood.Parasitemia ranges may determine practical limits of detection.

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Section: Specificity Of Molecular Assays and The Species Conceptmentioning

confidence: 75%

A review of Theileria diagnostics and epidemiology

Mans

Pienaar

Latif

2015

International Journal for Parasitology: Parasites and Wildlife

215

214

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“…Previous studies have reported Babesia in several species of African carnivores including domestic dogs, domestic cats, lions, cheetahs, wild cats (Felis silvestris ), caracal (Felis caracal ), and hyenas (Van den Berge 1937; Barnett and Brocklesby 1968;Collett 2000;Penzhorn et al 2001;Bosman et al 2007;Munson et al 2008; (Lopez-Rebollar et al 1999;Penzhorn et al 2001;Bosman et al 2007;Munson et al 2008;Githaka et al 2012). In the current study, lions were infected with three Babesia species; B. leo and B. felis were the two most common, and both of these Babesia spp.…”

Section: Discussionmentioning

confidence: 99%

“…The reason for differences in Babesia and Hepatozoon prevalence between hosts is unknown, primarily because very little is known about the life cycles, transmission routes, host specificity, or pathogenic potential of these carnivore parasites. Asymptomatic infections with Babesia and Hepatozoon wild African carnivores are common (McCully et al 1975;Averbeck et al 1990;Van Heerden et al 1995;LopezRebollar et al 1999;Penzhorn et al 2001;Penzhorn 2006;Bosman et al 2007;Bosman et al 2010;Githaka et al 2012); however, there are reports of disease in hyenas with Hepatozoon (East et al 2008) and Babesia can cause disease in lions if parasites are present in high numbers or if hosts are immunosuppressed or stressed (Penzhorn 2006;Munson et al 2008). Finally, a combination of morphological and molecular characterization of these parasites would greatly enhance our ability to compare contemporary studies with historical studies.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

et al. 2013

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A molecular survey was conducted for several hemoparasites of domestic dogs and three species of wild carnivores from two sites in Zambia. Three Babesia spp. were detected including Babesia felis and Babesia leo in lions (Panthera leo) and a Babesia sp. (similar to Babesia lengau) in spotted hyenas (Crocuta crocuta) and a single lion. All wild dogs (Lycaon pictus) and domestic dogs were negative for Babesia . High prevalences for Hepatozoon were noted in all three wild carnivores (38-61 %) and in domestic dogs (13 %). Significantly higher prevalences were noted in hyenas and wild dogs compared with domestic dogs and lions. All carnivores were PCR negative for Ehrlichia canis , Ehrlichia ewingii, and Bartonella spp. Overall, high prevalences and diversity of Babesia and Hepatozoon were noted in wild carnivores from Zambia. This study is the first molecular characterization of Babesia from any hyena species and is the first report of a Babesia sp. closely related to B. lengau, a parasite previously only reported from cheetahs (Acinonyx jubatus ), in lions and hyenas. Although usually benign in wild carnivores, these hemoparasites can be pathogenic under certain circumstances. Importantly, data on vectors for these parasites are lacking, so studies are needed to identify vectors as well as determine transmission routes, infection dynamics, and host specificity of these hemoparasites in wildlife in Africa and also the risk of transmission between domestic animals and wildlife.

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“…Babesia spp. have been reported in leopards (Panthera pardus), pampas cat (Leopardus colocolo), pumas, cheetahs, and lions (André et al, 2011;Averbeck et al, 1990;Ayoob et al, 2010;Bosman et al, 2007;Githaka et al, 2012;Kelly et al, 2014;Lopez-Rebollar et al, 1999;Penzhorn et al, 2001;Williams et al, 2014;Yabsley et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Is the free-ranging jaguar (Panthera onca) a reservoir for Cytauxzoon felis in Brazil?

Furtado

Taniwaki

Metzger

et al. 2017

Ticks and Tick-borne Diseases

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This study investigated the occurrence of Cytauxzoon felis and Babesia spp. in free-ranging jaguars (Panthera onca), domestic dogs (Canis lupus familiaris) and domestic cats (Felis catus) from the Cerrado, Amazon and Pantanal biomes of Brazil. Blood samples were collected from 30 jaguars, 129 dogs and 22 cats for detection of the 18S rRNA genes of piroplasmids. All of the jaguars from the Pantanal (n=22) and Cerrado (n=4) and three of four jaguars from the Amazon were positive for C. felis, but no dogs or cats were positive for the agent. All of the jaguars and domestic cats were negative for Babesia spp., while dogs from the Cerrado (7.9%; 5/63) and Amazon (10.6%; 5/47) biomes tested positive for the hemoparasite. Cytauxzoon nucleotide sequences detected were closely related to C. felis; and Babesia nucleotide sequences showed 100% of identity with Babesia vogeli. Although the pathogenicity of Cytauxzoon spp. genotypes that circulate in Brazil is still unknown, free-ranging jaguars probably play an important role in the maintenance of C. felis in nature. In addition, even though there is no evidence of the circulation of Babesia spp. between jaguars and dogs, the presence of this hemoparasite should be monitored in jaguar populations.

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Molecular detection and characterization of potentially new Babesia and Theileria species/variants in wild felids from Kenya

Cited by 16 publications

References 37 publications

A review of Theileria diagnostics and epidemiology

A review of Theileria diagnostics and epidemiology

Prevalence and diversity of Babesia, Hepatozoon, Ehrlichia, and Bartonella in wild and domestic carnivores from Zambia, Africa

Is the free-ranging jaguar (Panthera onca) a reservoir for Cytauxzoon felis in Brazil?

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