Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil

Comerlato, Juliana; Campos, Fabrício Souza; Oliveira, Martha Trindade; Cibulski, Samuel Paulo; Corrêa, Lilian; Kulmann, Marcos Iuri Roos; Arantes, Thalita Souza; Hentges, Lucas Peretti; Spilki, Fernando Rosado; Roehe, Paulo Michel; Franco, Ana Cláudia

doi:10.1002/jmv.24086

Cited by 17 publications

(15 citation statements)

References 50 publications

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“…The same subtypes of JCPyV (1 and 3) and the subgroup of BKPyV (I) characterized in the water samples were found in a previous study with urine samples collected in the same geographic region (Comerlato et al, 2015), reinforcing that the polyomaviruses detected in urban waters belong to the same subtypes that have been excreted by the local population. This is the first report on the presence and genetic diversity of BKPyV and JCPyV circulating in this region of the South of Brazil.…”

Section: Jcpyv and Bkpyv Characterizationsupporting

confidence: 79%

“…Asymptomatic infections are common in humans. Serological and virological studies show that more than 80% of adults harbor antibodies to BKPyV or JCPyV (Shah et al, 1973;Bodaghi et al, 2009;Egli et al, 2009;Khanna et al, 2009), and both viruses have been found in the urine of up to 53.4% of asymptomatic, immunocompetent individuals (Chehadeh et al, 2013;Comerlato et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

et al. 2016

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The human polyomaviruses JC and BK (JCPyV and BKPyV) are ubiquitous, species-specific viruses that belong to the family Polyomaviridae. These viruses are known to be excreted in human urine, and they are potential indicators of human wastewater contamination. In order to assess the distribution of both JCPyV and BKPyV in urban water samples collected from a sewage treatment plant (STP) and from a canalized water stream of Porto Alegre, Brazil, two nested-PCR assays were optimized and applied to the samples collected. The amplicons obtained were submitted to sequencing, and the sequences were analyzed with sequences of human polyomaviruses previously deposited in GenBank. Twelve out of 30 water samples (40%) were JCPyV positive, whereas six samples (20%) were BKPyV positive. The sequencing results confirmed the presence of JCPyV subtypes 1 and 3, whereas only BKPyV Ia and Ib were found. This study shows for the first time the presence of human polyomaviruses in surface water and in samples collected in a sewage treatment plant in southern Brazil.

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Section: Jcpyv and Bkpyv Characterizationsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

et al. 2016

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“…Особенно тяжелые проблемы ВК вирусы вызывают у пациентов на фоне лекарственного подавления иммунитета, когда происходит реактивация вирус ной инфекции с виремией и вирурией и развитием иногда фатальных осложнений. Особую актуаль ность представляет своевременная лабораторная диагностика ПВИ, направленная на оперативное выявление возбудителя и определение индивиду альной количественной оценки вирусной нагрузки с целью выработки адекватных подходов к лечению [12][13][14].…”

Section: Discussionunclassified

“…развитие криза отторжения, лимфопролифератив ных заболеваний у пациентов с трансплантирован ной почкой [8][9][10][11][12][13][14][15]. H. Shimohata и соавт.…”

Section: оценка вклада герпесвирусной инфекции в развитие и прогрессиunclassified

Оцінка Вкладу Герпесвірусної Інфекції В Розвиток І Прогресування Хронічних Гломерулярних Хвороб У Дітей

Kazyra¹,

Rubanik²,

Sukala³

et al. 2021

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Êë³í³÷íà ïåä³àòð³ÿ / Clinical Pediatrics ® Введение Этиология хронических болезней клубочково го аппарата почки на сегодняшний день до конца не известна. Описано участие ряда факторов, в том числе вирусных инфекций [1-5]. Внимание к роли вирусов гепатита В и С в развитии гломерулонеф рита знаменовало начало изучения вирусассоци ированных нефропатий [2]. По данным литературы прослежена связь между инфекцией, вызванной вирусами семейства герпесвирусов (вирус простого герпеса (ВПГ), Эпштейна-Барр (ВЭБ), цитоме галовирус (ЦМВ)) и развитием как самого заболе вания, так и провокацией обострений [4-7]. Среди приведенных-нефротический синдром, систем ные заболевания соединительной ткани (системная красная волчанка (СКВ)), ревматоидный артрит, системные васкулиты (IgAваскулит Шенлейна-Геноха (ШГ), гранулематоз Вегенера и др.), а также

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“…More recent publications on multiplex PCR for both viruses are based on the methodology described by Fedele et al 19 which can, as shown, generate non-specific results, and also the methodology proposed by Bofil-Mas et al . 20 .…”

mentioning

confidence: 99%

A globally applicable PCR-based detection and discrimination of BK and JC polyomaviruses

Souza¹,

Savassi‐Ribas

Almeida³

et al. 2018

Rev. Inst. Med. trop. S. Paulo

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BKV and JCV belong to the Polyomaviridae family and are opportunistic agents associated with complications in immunocompromised individuals. Although a single screening assay for both viruses would be convenient, the diversity of BKV and JCV serotypes and genotypes is a methodological challenge. In this paper, we developed a PCR method able to detect and segregate BKV and JCV, despite these genetic discrepancies. A duplex semi-nested PCR (duplex snPCR) was designed to target a conserved region (639nt-1516nt) within the VP2 gene. In the first PCR, a primer set common to all BKV and JCV serotypes/ genotypes was used, followed by a semi-nested PCR with internal primers for BKV and JCV segregation. The limit of detection of the duplex snPCR was as low as 10 copies of BKV or JCV plasmids/μL. Specific products were observed when JCV and BKV plasmids were mixed in the same reaction. In field sample testing, the duplex snPCR detected and distinguished both viruses in different biological samples. Results were confirmed by Sanger's sequencing. The geographical complexity of BKV and JCV serotypes and genotypes imposes limits to a simple and universal method that could detect each virus. However, we describe here a sensitive and reliable PCR technique for BKV and JCV diagnosis that overcomes these limitations and could be universally applied.

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Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil

Cited by 17 publications

References 50 publications

Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil

Оцінка Вкладу Герпесвірусної Інфекції В Розвиток І Прогресування Хронічних Гломерулярних Хвороб У Дітей

A globally applicable PCR-based detection and discrimination of BK and JC polyomaviruses

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