Molecular detection and characterisation of Orf5 Gene of porcine reproductive and respiratory syndrome virus from outbreaks in Mizoram

Isolation and molecular characterization of GP5 glycoprotein gene of Betaarterivirus suid 2 from Mizoram, India

“…Since ORF5 is the most variable region containing both neutralizing and non-neutralizing epitopes, N-linked glycosylation sites, apoptotic cell death induced region; so phylogenetic differences are more prominent in this region [ 12 , 14 , 26 ]. The close similarity of Indian strains and their relation with other highly pathogenic strains were also reported by Rajkhowa et al [ 28 ]; Zohlimpuia et al [ 42 ]; Chaithra et al [ 4 ]. If we observe the timeline of the highly pathogenic strain of Betaarterivirus suid 2 outbreaks in the south-east Asian region then it can be assumed that the virus has entered Mizoram from neighboring Myanmar.…”

“…Similarly compared with highly pathogenic 10 HEB-3 strain and 2013 outbreak was 98.33–99.17%. In another observation by Zohlimpuia et al [ 42 ] reported 98.3–100% similarity among the four isolates of the 2016 outbreak in Mizoram, 98.2–99.7% similarity with Meghalaya isolates. He also observed 98–99% sequence similarity with the highly pathogenic strains of the 2011 outbreak in Myanmar.…”

“…Isolation of the virus was performed using archival tissue samples preserved in the Department of Veterinary Microbiology from the previous outbreaks of PRRS in Mizoram, India reported by Zohlimpuia et al [ 42 ]. The archival samples used are lung and spleen tissues of dead piglets which were collected in the 2016 PRRS outbreak from Lawngtlai and Saiha districts of Mizoram.…”

“…Zohlimpuia et al [42]; Chaithra et al [4]. If we observe the timeline of the highly pathogenic strain of Betaarterivirus suid 2 outbreaks in the south-east Asian region then it can be assumed that the virus has entered Mizoram from neighboring Myanmar.…”

“…ORF5 sequence is used for phylogenetic study and virus characterization [ 5 ]. Since its first outbreaks in 2013 in Mizoram, subsequent years in 2015, 2016, and 2018 outbreaks of the disease have been reported [ 29 , 30 , 42 ]. This study was designed to isolate the virus from an outbreak in Mizoram, India, and to study the GP5 gene sequence.…”