Molecular detection and characterisation of Horsegram Yellow Mosaic Virus (HgYMV) infecting Lima bean (Phaseolus lunatus) in India

Abarshi, M. M.; Abubakar, A. L.; Garba, A.; Mada, S. B.; Ibrahim, A. B.; Maruthi, M. N.

doi:10.4314/v33i1.6

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…comparison of nucleotide distances for DNA-B component of HgYMV1 indicated that it was Table 3: Percent nucleotide identities for the complete DNA-B genome and genes of Indian HgYMV with selected begomoviruses Discussion genome organisation of (HgYMV1 and HgYMV2) HgYMV were detected successfully using reported in this study was similar to that of a degenerate primers Deng A/B in the typical bipartite genome reported by previous symptomatic plant leaves samples. The studies (Abarshi et al, 2017;Rienzie et al, 2016; presence of these viruses (HgYMV1 and Abarshi et al, 2017). HgYMV2) was confirmed by PCR.…”

Section: Introductionmentioning

confidence: 86%

Molecular identification and sequence analysis of bipartite Begomovirus infecting Horsegram legume in India

Abubakar¹,

Abarshi²,

Maruthi³

2019

Nig. J. Biotechnol

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The molecular diversity of Begomovirus infecting Horsegram yellow Mosaic viruses (HgYMV1 and HgYMV2), from two main horsegram growing farms near Bangalore, Karnataka State, South India was investigated. The viral DNA was amplified from horsegram plants exhibiting mild and severe symptoms by polymerase chain reaction, and complete genome of the HgYMV were identified by their sequence analysis. Isolates of HgYMV1 and HgYMV2 were found to be associated with severe symptom phenotype from HgYMV. HgYMV was most closely related to Mungbean yellow mosaic indian viruse (MYMIV) and Mungbean yellow mosaic virus (MYMV) at 81.8 to 84.8 % nucleotide identity, based on DNA-A and DNA-B component sequences. HgYMV was distantly related to Dolicos yellow mosaic virus from Asia (DoYMV-Ban and DoYMV-DB) and partially to Cowpea golden mosaic virus from Nigeria (CPGMV-[NG]) at 64 and 62 % DNA nucleotide identity. Analysis of the DNA-B sequence of HgYMV revealed a DNA-B component identical to those of Bean golden yellow mosaic virus BGYMV isolates described. Furthermore, the DNA-B component for extant BGYMV isolates and strains were also the closest relatives for the HgYMV1 DNA-B components at 48.7 % nucleotide identity. Therefore, HgYMV could be considered to be a new species of the genus Begomovirus (family Geminiviridae).

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Section: Introductionmentioning

confidence: 86%

Molecular identification and sequence analysis of bipartite Begomovirus infecting Horsegram legume in India

Abubakar¹,

Abarshi²,

Maruthi³

2019

Nig. J. Biotechnol

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“…For instance, Rienzie et al (2017) detected HgYMV in soybean (Glycine max) plants grown in experimental Hedyotis corymbosa as a weed that harboured HgYMV (Rienzieet al, 2016). Moreover,Abarshi et al (2017) detected the same virus in lima bean (Phaseolus lunatus) in Bangalore, India. Although only a minimum number…”

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confidence: 89%

Transmission and host range of Horsegram yellow mosaic virus (HgYMV) causing common bean (Phaseolus vulgaris L.) yellowing disease in Sri Lanka

Rienzie

Costa

Wickramaarachchi³

2020

J. Natn. Sci. Foundation Sri Lanka

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The common bean (Phaseolus vulgaris L.) yellowing disease (BYD) is caused by the Horsegram yellow mosaic virus (HgYMV), which is a begomovirus transmitted Bemisia tabaci Genn.). It is a serious threat to the common bean cultivation in Sri Lanka. Despite the long time, its etiology and virus vector relationships are not understood well. Therefore, the present work was carried out to identify major aspects of the etiology of the virus. Initially a survey was conducted to determine the prevalence of the virus in representative bean growing locations in Sri Lanka, followed by a host range study using 54 plant species. Characteristics of virus-vector relationships were determined by conducting a series of virus transmission tests under glasshouse conditions. The survey results revealed the incidence of BYD at some time or the other in most of the sampled localities, which are scattered around all four major districts where the common bean is grown. Through the host range study, it was demonstrated that Ageratum sp., Glycine max Merrill., (Lam.) Verdc., and Phaseolus lunatus L. are susceptible to HgYMV, indicating a narrow host range. In addition to the symptomatology, the successful entry of the virus following 96.7 %, which corresponds to 0.24 of the estimated probability could be transmitted through probing by a single viruliferous acquisition access period and inoculation access period were 20 min in each case and the respective disease incidence values could retain the virus in an active state for up to 9 days. : Begomovirus, Bemisia tabaci Genn., common bean yellowing disease, Horsegram yellow mosaic virus (HgYMV), host range, virus transmission.

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Phaseolus lunatus (Lima bean)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

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Molecular detection and characterisation of Horsegram Yellow Mosaic Virus (HgYMV) infecting Lima bean (Phaseolus lunatus) in India

Cited by 3 publications

References 4 publications

Molecular identification and sequence analysis of bipartite Begomovirus infecting Horsegram legume in India

Molecular identification and sequence analysis of bipartite Begomovirus infecting Horsegram legume in India

Transmission and host range of Horsegram yellow mosaic virus (HgYMV) causing common bean (Phaseolus vulgaris L.) yellowing disease in Sri Lanka

Phaseolus lunatus (Lima bean)

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