Molecular Detection and Biological Control of  Human Hair Dandruff Causing Microorganism Staphylococcus aureus

Mas-ud, Md. Atik; Dna, Joarder; Ali, Roushan; Hasan, Sharizal; Islam, Ashikul; Hasan, Md. Faruk; Islam, Md. Asadul; Sikdar, Biswanath

doi:10.22207/jpam.14.1.16

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…To identify the obtained bacteria, all pure isolates were first incubated in R2A broth for 24 h, and then, genomic DNA for each pure isolate was extracted using a DNA extraction kit (Magen Biotech, Guangzhou, China). Subsequently, the 16S rRNA gene was amplified in a 25-μl volume using universal primers 27 F and1492 R ( Mas-Ud et al, 2020 ), and amplification was performed as previously described ( Gu et al, 2018 ). Then, PCR products were sequenced by BGI Tech Solutions Co., Ltd (Guangzhou, China), and 16S rRNA gene sequences were submitted to the NCBI public library of bioinformatics for comparison and identification.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Antibiotic Resistance Genes and Antibiotic-Resistant Bacteria in Full-Scale Drinking Water Treatment System Using Metagenomics and Culturing

Sun

Lin

et al. 2022

Front. Microbiol.

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The contamination of antibiotic resistance genes (ARGs) may directly threaten human health. This study used a metagenomic approach to investigate the ARG profile in a drinking water treatment system (DWTS) in south China. In total, 317 ARG subtypes were detected; specifically, genes encoding bacitracin, multidrug, and sulfonamide were widely detected in the DWTS. Putative ARG hosts included Acidovorax (6.0%), Polynucleobacter (4.3%), Pseudomonas (3.4%), Escherichia (1.7%), and Klebsiella (1.5%) as the enriched biomarkers in the DWTS, which mainly carried bacitracin, beta-lactam, and aminoglycoside ARGs. From a further analysis of ARG-carrying contigs (ACCs), Stenotrophomonas maltophilia and Pseudomonas aeruginosa were the most common pathogens among the 49 ACC pathogens in the DWTS. The metagenomic binning results demonstrated that 33 high-quality metagenome-assembled genomes (MAGs) were discovered in the DWTS; particularly, the MAG identified as S. maltophilia-like (bin.195) harbored the greatest number of ARG subtypes (n = 8), namely, multidrug (n = 6; smeD, semE, multidrug_transporter, mexE, semB, and smeC), beta-lactam (n = 1; metallo-beta-lactamase), and aminoglycoside [n = 1; aph(3’)-IIb]. The strong positive correlation between MGEs and ARG subtypes revealed a high ARG dissemination risk in the DWTS. Based on the pure-culture method, 93 isolates that belong to 30 genera were recovered from the DWTS. Specifically, multidrug-resistant pathogens and opportunistic pathogens such as P. aeruginosa, Bacillus cereus, and S. maltophilia were detected in the DWTS. These insights into the DWTS’s antibiotic resistome indicated the need for more comprehensive ARG monitoring and management in the DWTS. Furthermore, more effective disinfection methods need to be developed to remove ARGs in DWTSs, and these findings could assist governing bodies in the surveillance of antibiotic resistance in DWTSs.

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Section: Methodsmentioning

confidence: 99%

Characteristics of Antibiotic Resistance Genes and Antibiotic-Resistant Bacteria in Full-Scale Drinking Water Treatment System Using Metagenomics and Culturing

Sun

Lin

et al. 2022

Front. Microbiol.

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“…Genomic DNA was extracted from the liquid culture of isolated pure bacterial strains. Genomic DNA was extracted using CTAB (Cetyl Trimethyl Ammonium Bromide) buffer method according to Mas-Ud et al (2020) . Extracted genomic DNA was purified according to the kit instructions (Thermo Fisher Scientific, DNA Purification Kit #K0721).…”

Section: Methodsmentioning

confidence: 99%

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

Hossain

Billah

Ali

et al. 2021

Saudi Journal of Biological Sciences

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Ralstonia solanacearum is a harmful pathogen that causes severe wilt disease in several vegetables. In the present study, we identified R. solanacearum from wilt of papaya by 16S rRNA PCR amplification. Virulence ability of R. solanacearum was determined by amplification of approximately 1500 bp clear band of hrpB gene. Further, in-vitro seed germination assay showed that R. solanacearum reduced the germination rate up to 26.21%, 34% and 33.63% of cucumber, bottle guard and pumpkin seeds, respectively whereas shoot and root growth were also significantly decreased. Moreover, growth inhibition of R. solanacearum was recorded using antibacterial compound from medicinal plant and antagonistic B. subtilis. Petroleum ether root extract of Rauvolfia serpentina showed highest 22 ± 0.04 mm diameter of zone of inhibition where methanolic extract of Cymbopogon citratus and ethanolic extract of Lantana camara exhibited 20 ± 0.06 mm and 20 ± 0.01 mm zone of inhibition against R. solanacearum , respectively . In addition, bioactive compounds of B. subtilis inhibited R. solanacearum growth by generating 17 ± 0.09 mm zone of inhibition. To unveil the inhibition mechanism, we adopted chemical-protein interaction network and molecular docking approaches where we found that, rutin from C. citratus interacts with citrate (Si)-synthase and dihydrolipoyl dehydrogenase of R. solanacearum with binding affinity of −9.7 kcal/mol and −9.5 kcal/mol while quercetin from B. subtillis interacts with the essential protein F0F1 ATP synthase subunit alpha of the R. solancearum with binding affinity of −6.9 kcal/mol and inhibit the growth of R. solanacearum . Our study will give shed light on the development of eco-friendly biological control of wilt disease of papaya.

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“…Furthermore, research in yeast had focused on the ability of cell wall glycoproteins, such as mannoproteins, to adsorb harmful medium-chain fatty acids (MCFAs) and phenolic chemicals [50]. Many attempts have been made to use POME as a medium for microbial production [40,44,[51][52][53][54], but an application employing yeast-bacteria co-culture for CBL synthesis using the oil content in this wastewater as part of a combined biotreatment method has yet to be discovered (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

Fibriana

Upaichit

Cheirsilp

2021

Biomass Conv. Bioref.

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Two bacterial and six yeast strains capable of lipase production were investigated for their extracellular lipase (ECL), cellbound lipase (CBL), and growth profile in basal standard medium (BSM) and the low-cost palm oil mill effluent (POME) media. The yeast Magnusiomyces spicifer AW2 and bacteria Staphylococcus hominis AUP19 were selected and further employed to explore their survivability, lipase activity, and cell biomass production in various POME concentrations. It was revealed that diluted POME 1:1 at pH 7.0 was suitable for yeast and bacterial performance. Furthermore, the development of co-culture between yeast and bacteria was performed by mixing them at a ratio of 1:1. The synthetic co-culture system was successful in improving CBL activity to 3860 U/L. The bioremediation parameter, i.e., oil and grease (O&G) removal, was efficiently achieved 1.1-fold higher than yeast alone and bacterial monoculture at 80.1 ± 1.3%. At the same time, COD removal was highest with the co-culture treatment of 75.9 ± 2.8%, indicating 1.3-fold more effective than yeast monoculture and 1.4-fold better than single bacterial form. The mixed cells of two strains effectively improved biodiesel production from oleic acid of 73.5 ± 3.3% and palm oil of 82.5 ± 0.3% under solvent-free conditions for 72-h reactions at room temperature (30 ± 2 °C), indicating a promising prospect of a combined approach of POME treatment and valorization.

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Molecular Detection and Biological Control of Human Hair Dandruff Causing Microorganism Staphylococcus aureus

Cited by 3 publications

References 18 publications

Characteristics of Antibiotic Resistance Genes and Antibiotic-Resistant Bacteria in Full-Scale Drinking Water Treatment System Using Metagenomics and Culturing

Characteristics of Antibiotic Resistance Genes and Antibiotic-Resistant Bacteria in Full-Scale Drinking Water Treatment System Using Metagenomics and Culturing

Molecular identification and biological control of Ralstonia solanacearum from wilt of papaya by natural compounds and Bacillus subtilis: An integrated experimental and computational study

Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

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