Molecular detection and antibiogram of Salmonella spp. from apparently healthy Japanese quails of three different quail farms in Mymensingh

Abstract

Objective: The present study was carried out for the isolation, identification and antibiogram study of Salmonella spp. from apparently healthy Japanese quails (Coturnix japonica) at three different quail farms in Mymensingh, Bangladesh. Materials and methods: A total of 75 cloacal swab samples were randomly collected from apparently healthy Japanese quails from three different farms at Mymensingh, Bangladesh. The samples were subjected to a series of cultural and biochemical examination for the isolation of Salmonella followed by molecular detection by polymerase chain reaction (PCR). Motility of the Salmonella was performed by motility test and amplification of speF gene. The antibiogram profile of the isolates was also evaluated against commonly used antimicrobials by disc diffusion method. Results: The overall prevalence of Salmonella spp. in quails was found to be 13.33%(n=10/75). Out of the 10 isolates, seven were found to be motile. Farmwise, the prevalence of Salmonella spp. were 10%(n=3/30), 24%(n=6/25) and 5%(n=1/20) at the quail farms of Bangladesh Agricultural University (BAU), Shikarikanda and Akua, respectively. The antibiogram study revealed that all the isolates were resistant to both Erythromycin and Tetracycline. On the other hand, 100% isolates were sensitive to both Ciprofloxacin and Imipenem. Ninety percent isolates of Salmonella were resistant to Colistin sulphate. Neomycin was found to be sensitive to 80% Salmonella isolates. All the Salmonella isolates were found to be multidrug resistant (MDR). Conclusion:The presence of MDR Salmonella spp. in quails signifies public health importance of the organisms, which may be associated with food-borne illness.

Phenotypic and Genotypic Characterisation of ESBL Producing and Carbapenem-resistant Escherichia coli and Salmonella spp. in Japanese Quail Farms of Wayanad District

“…Out of the 22 quail cloacal samples analysed, two isolates (9.09 per cent) were positive for Salmonella spp. and the findings were similar to the nine per cent,11.11 per cent and 13.3 per cent reported by Awadallah et al, (2013); Palanisamy and Bamaiyi (2015) and Jahan et al, (2018), respectively in the earlier studies.…”

Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons

“…The prevalence of Salmonella spp. in samples taken from seemingly healthy quails from Mymensingh was reported to be 13.33% [35]. Furthermore, a study on 400 pigeon samples was conducted in Egypt and reported 5%, 3.5%, and 4.6% prevalence of Salmonella spp.…”

A Comprehensive Review on the Common Emerging Diseases in Quails.

“…Moreover, it was found that 10 out of 75 (13.33%) quail samples from three farms in Bangladesh were positive for Salmonella spp. of which seven isolates were motile Salmonella (Jahan et al, 2018). Barde (2014) demonstrated that S. Gallinarum in Japanese quails causes septicemic disease with distribution of the organism in major organs, greenish-yellow diarrhea, high mortality, marked drop in egg production and congestion with enlargement of internal organs, which is similar to that in chickens.…”

“…Nowadays, in-ovo inoculation of antibiotics is considered a new trend to prevent the possibility of bacterial pathogen transmission through eggs (Tavakkoli and Gooshki, 2014). In a study by Jahan et al (2018), the in-vitro antibiotic sensitivity test of Salmonella strains isolated from quails showed that 100% of strains were resistant to erythromycin and tetracycline, but were sensitive to ciprofloxacin and imipenem, 90% of strains were resistant to colistin sulfate and 80% were sensitive to neomycin. Also, all Salmonella isolates showed multidrug resistance.…”