Molecular details on gilthead sea bream (Sparus aurata) sensitivity to low water temperatures from 1H NMR metabolomics

Melis, Riccardo; Sanna, Roberta; Braca, Angela; Bonaglini, Elia; Cappuccinelli, Roberto; Slawski, Hanno; Roggio, Tonina; Uzzau, Sergio; Anedda, Roberto

doi:10.1016/j.cbpa.2016.11.010

Cited by 35 publications

(36 citation statements)

References 45 publications

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“…In summary, seasonal and latitudinal changes on the metabolome, ionome and microbiome of wild yellowfin goby were observed and their potential interaction with the estuarine environment such as temperature might play an important role in body homeostasis of wild yellowfin goby. In a previous study 56 , NMR-based metabolic analysis of gilthead sea bream under non-isothermal conditions revealed that muscle lipid production such as cholesterol, phosphocholine, and fatty acids provided energy source for the ectothermic fish in response to sudden ambient temperature change. Our results further demonstrate that symbiotic intestinal microbiota is more sensitive than their host to the environment, such as the temperature.…”

Section: Discussionmentioning

confidence: 97%

Systemic Homeostasis in Metabolome, Ionome, and Microbiome of Wild Yellowfin Goby in Estuarine Ecosystem

Wei

Sakata

Asakura

et al. 2018

Sci Rep

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Data-driven approaches were applied to investigate the temporal and spatial changes of 1,022 individuals of wild yellowfin goby and its potential interaction with the estuarine environment in Japan. Nuclear magnetic resonance (NMR)-based metabolomics revealed that growth stage is a primary factor affecting muscle metabolism. Then, the metabolic, elemental and microbial profiles of the pooled samples generated according to either the same habitat or sampling season as well as the river water and sediment samples from their habitats were measured using NMR spectra, inductively coupled plasma optical emission spectrometry and next-generation 16 S rRNA gene sequencing. Hidden interactions in the integrated datasets such as the potential role of intestinal bacteria in the control of spawning migration, essential amino acids and fatty acids synthesis in wild yellowfin goby were further extracted using correlation clustering and market basket analysis-generated networks. Importantly, our systematic analysis of both the seasonal and latitudinal variations in metabolome, ionome and microbiome of wild yellowfin goby pointed out that the environmental factors such as the temperature play important roles in regulating the body homeostasis of wild fish.

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Section: Discussionmentioning

confidence: 97%

Systemic Homeostasis in Metabolome, Ionome, and Microbiome of Wild Yellowfin Goby in Estuarine Ecosystem

Wei

Sakata

Asakura

et al. 2018

Sci Rep

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“…When environmental temperature decreased to 8°C, however, the energy utilization strategy of sea bream changed to the production of nonpolar and polar lipids, whereas the glycogen content in the livers was not significantly changed. In environments with much lower temperatures, the production of lipids was altered to maintain membrane fluidity, and glycogen storage decreased in the liver of the sea bream (Ibarz et al, 2010a ; Melis et al, 2017 ). Being a tropical and herbivorous species, the milkfish cannot survive in temperatures lower than 15°C and continue to use carbohydrates as their main energy resource (Chiu and Benitez, 1981 ; Benitez, 1983 ; Hu et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Salinity Effects on Strategies of Glycogen Utilization in Livers of Euryhaline Milkfish (Chanos chanos) under Hypothermal Stress

et al. 2018

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The fluctuation of temperature affects many physiological responses in ectothermic organisms, including feed intake, growth, reproduction, and behavior. Changes in environmental temperatures affect the acquisition of energy, whereas hepatic glycogen plays a central role in energy supply for the homeostasis of the entire body. Glycogen phosphorylase (GP), which catalyzes the rate-limiting step in glycogenolysis, is also an indicator of environmental stress. Here, we examined the effects of salinity on glycogen metabolism in milkfish livers under cold stress. A reduction of feed intake was observed in both freshwater (FW) and seawater (SW) milkfish under cold adaptation. At normal temperature (28°C), compared to the FW milkfish, the SW milkfish exhibited greater mRNA abundance of the liver isoform of GP (Ccpygl), higher GP activity, and less glycogen content in the livers. Upon hypothermal (18°C) stress, hepatic Ccpygl mRNA expression of FW milkfish surged at 3 h, declined at 6 and 12 h, increased again at 24 h, and increased significantly after 96 h. Increases in GP protein, GP activity, and the phosphorylation state and the breakdown of glycogen were also found in FW milkfish livers after 12 h of exposure at 18°C. Conversely, the Ccpygl transcript levels in SW milkfish were downregulated after 1 h of exposure at 18°C, whereas the protein abundance of GP, GP activity, and glycogen content were not significantly altered. Taken together, under 18°C cold stress, FW milkfish exhibited an acute response with the breakdown of hepatic glycogen for maintaining energy homeostasis of the entire body, whereas no change was observed in the hepatic glycogen content and GP activity of SW milkfish because of their greater tolerance to cold conditions.

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“…To visualize the data set of P. plecoglossicida samples and identify an overview clustering trend, the principal component analysis (PCA) was performed. First, the significantly changed metabolites were determined by Student's t test (|fold change|≥1.5, FDR≤0.5) (Melis et al, ). Then, SIMCA‐P 12.0 software (Umetrics AB, Umeå, Sweden) was used to perform the partial least squares discriminant analysis (PLS‐DA), in order to explain the status of P. plecoglossicida in different temperatures from all the ions constituting the fingerprint and for validation by performing permutation tests (Kopka et al, ).…”

Section: Methodsmentioning

confidence: 99%

A metabolomic investigation into the temperature‐dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)

Huang

Zuo

Jiang

et al. 2019

Journal of Fish Diseases

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Pseudomonas plecoglossicida is associated with multiple fish diseases, and temperature is one of the most important environmental factors related to its outbreak. To elucidate the influence of temperature variation on the pathogen, the global metabolomics of P. plecoglossicida (NZBD9) were analysed at the virulent (18°C) and avirulent (12°C and 28°C) temperatures. The result showed that the levels of Phosphoric acid, Tyrosine, Spermidine and Sucrose were significantly reduced，while Itaconic acid, Glucaric acid and Isomaltose were increased in P. plecoglossicida at 18°C. These metabolic adjustments assist P. plecoglossicida to survive in adverse environments, proliferate in the host, colonize and resist host immune clearance during the initial steps of infection. The results suggested that L321_03626 and L321_18122 genes played a key role in the regulation of these metabolic adaptions and thus regulated P. plecoglossicida virulence at virulent temperature, which was proved by further gene silencing and artificial infection. The present study, for the first time, determines the P. plecoglossicida metabolomic responses to temperature variation, which is helpful to explore its pathogenic mechanism and provides reference for disease control.

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Molecular details on gilthead sea bream (Sparus aurata) sensitivity to low water temperatures from 1H NMR metabolomics

Cited by 35 publications

References 45 publications

Systemic Homeostasis in Metabolome, Ionome, and Microbiome of Wild Yellowfin Goby in Estuarine Ecosystem

Systemic Homeostasis in Metabolome, Ionome, and Microbiome of Wild Yellowfin Goby in Estuarine Ecosystem

Salinity Effects on Strategies of Glycogen Utilization in Livers of Euryhaline Milkfish (Chanos chanos) under Hypothermal Stress

A metabolomic investigation into the temperature‐dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)

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