Molecular design of novel pH/temperature-sensitive hydrogels

Huynh, Dai Phu; Nguyen, Minh Khanh; Kim, Bong Sup; Lee, Sang Soo

doi:10.1016/j.polymer.2009.03.060

Cited by 49 publications

(49 citation statements)

References 18 publications

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“…[82] Thus, it was important to control this shift near physiological conditions for practical applications. [84] The gel window could be tailored by varying the PEG molecular weight, PAE block length, PCL/PEG ratio, and concentration. In addition, the degradation of pentablock copolymers could be controlled by substituting PCLA for the PCL block.…”

Section: Ph/temperature-sensitive Block Copolymer Hydrogelsmentioning

confidence: 99%

Injectable Biodegradable Hydrogels

Nguyen

Lee

2010

Macromolecular Bioscience

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Injectable biodegradable copolymer hydrogels, which exhibit a sol–gel phase transition in response to external stimuli, such as temperature changes or both pH and temperature (pH/temperature) alterations, have found a number of uses in biomedical and pharmaceutical applications, such as drug delivery, cell growth, and tissue engineering. These hydrogels can be used in simple pharmaceutical formulations that can be prepared by mixing the hydrogel with drugs, proteins, or cells. Such formulations are administered in a straightforward manner, through site‐specific control of release behavior, and the hydrogels are compatible with biological systems. This review will provide a summary of recent progress in biodegradable temperature‐sensitive polymers including polyesters, polyphosphazenes, polypeptides, and chitosan, and pH/temperature‐sensitive polymers such as sulfamethazine‐, poly(β‐amino ester)‐, poly(amino urethane)‐, and poly(amidoamine)‐based polymers. The advantages of pH/temperature‐sensitive polymers over simple temperature‐sensitive polymers are also discussed. A perspective on the future of injectable biodegradable hydrogels is offered.magnified image

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Section: Ph/temperature-sensitive Block Copolymer Hydrogelsmentioning

confidence: 99%

Injectable Biodegradable Hydrogels

Nguyen

Lee

2010

Macromolecular Bioscience

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416

343

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“…[18] Briefly, the core triblock (PCL-PEG-PCL) was synthesized by ring-opening polymerization of CL using bis(hydroxyl)PEG as a macroinitiator in the presence of stannous octoate as a catalyst. The hydroxyl groups at both ends of the triblock copolymer were modified in anhydrous chloroform with acryloyl chloride to generate acrylate groups.…”

Section: Synthesis Of Pae-pcl-peg-pcl-pae Copolymermentioning

confidence: 99%

“…The resulting polymer solution was evaporated to remove methylene chloride and the dry residue was dissolved in tetrahydrofuran (THF), filtered through filter paper (5C 100 circles; Toyo Roshi Kaisha, Japan) to remove PAE homopolymer, further purified by precipitation in diethyl ether, and dried under reduced pressure. [18,19] The pentablock copolymer was characterized by using 1 H NMR and gel permeation chromatography (GPC). The composition of the triblock PCL-PEG-PCL and pentablock copolymer obtained by calculating the relative areas of corresponding peaks in 1 H NMR spectrum and GPC, respectively, as our previous report.…”

Section: Synthesis Of Pae-pcl-peg-pcl-pae Copolymermentioning

confidence: 99%

“…[16] The molecular weight of obtained pentablock copolymer was 7 500 (PAE-PCL-PEG-PCL-PAE ¼ 1 300-1 600-1 700-1 600-1 300). [18] The stimuli-responsive phase transition behavior of the copolymer was determined as described in our previous publications. [18][19][20] Cell Culture Human embryonic kidney cells (HEK293), mouse insulinoma cells (Min6), and murine fibroblast (NIH 3T3) were cultured in DMEM supplemented with 10% FBS, streptomycin at 100 mg Á mL À1 , penicillin at 100 IU Á mL À1 , and 2 Â 10 À3 M L-glutamine.…”

Section: Synthesis Of Pae-pcl-peg-pcl-pae Copolymermentioning

confidence: 99%

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pH‐Sensitive Pentablock Copolymer Nanocapsules as Nontoxic and Efficient Gene Carriers

Lee

Jang

Huynh

et al. 2011

Macromolecular Bioscience

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A biodegradable amphiphilic pentablock copolymer PAE-PCL-PEG-PCL-PAE with a pH-sensitive unit was synthesized for use as a nontoxic, biodegradable carrier for gene delivery by forming nanocapsules entrapping nucleic acid drugs. The PAE block can interact with plasmid DNA to form polyelectrolyte complexes in an acidic environment. At physiological pH, the PAE blocks are deprotonated and form an insoluble skin, resulting in the formation of nanocapsules that encapsulate plasmid DNA. The surface charges of the nanocapsules became almost neutral at pH = 7.4, and their size ranged from 210 to 280 nm. The nanocapsule maintained most of its transfection efficiency even in the presence of serum. These nanocapsules are therefore potential carriers for systemic gene therapy.

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“…In recent years, stimuli-sensitive hydrogels which exhibit phase transition behaviors have been widely noticed [9][10][11][12]. For example, Park et al [13] used poly (N-isopropylacrylamide) and its copolymer hydrogels for enzyme immobilization, and found that the catalytic activity of the enzyme could be modulated by the change of temperature.…”

Section: Introductionmentioning

confidence: 99%

Temperature-sensitive molecularly imprinted microgels with esterase activity

2011

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Temperature-sensitive molecularly imprinted microgels (MIGs) exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate (PAD-His) as the functional macromonomer and p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) as well as Co 2+ as the coordination center. The catalytic activity of MIGs was greatly influenced by the amount of the template, and could be modulated by temperature. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The MichaelisMenten constant and maximum velocity were found to be 2.2 × 10 5 mol/L and 2.04 × 10 8 mol/h, respectively. In addition, the MIGs were found to have a high catalytic selectivity to NPA. molecular imprinting, microgels, esterase, temperature sensitivity, dextran

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Molecular design of novel pH/temperature-sensitive hydrogels

Cited by 49 publications

References 18 publications

Injectable Biodegradable Hydrogels

Injectable Biodegradable Hydrogels

pH‐Sensitive Pentablock Copolymer Nanocapsules as Nontoxic and Efficient Gene Carriers

Temperature-sensitive molecularly imprinted microgels with esterase activity

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