Molecular Description of Proclotting Enzyme: A Power Tool for Insect Biological Control of Aedes aegypti L.1

Villanueva-Segura, Olga Karina; Gómez-Govea, Mayra A.; Garza‐Veloz, Idalia; González-Álvarez, Rafael; Carrillo-Gaytán, Diego; Ramírez-Valles, Eda Guadalupe; Martínez-Dávila, Jorge A.; Fierro, Norma Cruz; Trujillo-Murillo, Karina del Carmen; Martínez‐Fierro, Margarita L.; Delgado‐Enciso, Iván; Rodríguez‐Sánchez, Iram P.; Guzmán-Velasco, Antonio

doi:10.3958/059.045.0312

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…The inhibition of histone acetylation using C646 had more severe consequences on gene expression in worker fat bodies compared to the inhibition of histone deacetylation using TSA, shifting the expression of nearly 10 times as many genes. Among the most strongly downregulated genes in ants treated with the chemical inhibitors, we found interesting candidates such as the Indy protein, which has been linked to longevity in Drosophila (Rogina & Helfand, 2013), the enzyme proclotting, which plays a role in the innate immune response (Villanueva-Segura et al, 2020), or a gene associated with juvenile hormone, which is involved in many physiological processes including aging (Yamamoto et al, 2013). We also detected seven downregulated genes associated with the fatty-acid synthesis in the queenless, C646-treated group compared to only one in the queenless control.…”

Section: Discussionmentioning

confidence: 99%

Histone acetylation regulates the expression of genes involved in worker reproduction and lifespan in the ant Temnothorax rugatulus

Choppin

Feldmeyer

Foitzik³

2021

Preprint

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In insect societies, the queen monopolizes reproduction while workers perform tasks such as brood care or foraging. Queen loss leads to ovary development and lifespan extension in workers from many ants. However, the underlying molecular mechanisms of this phenotypic plasticity remain unclear. Recent studies highlight the importance of epigenetics in regulating plastic traits in social insects. We investigated the role of histone acetylation in the regulation of worker reproduction in the ant Temnothorax rugatulus. We removed queens from their colonies to induce worker fecundity, and either fed workers with chemical inhibitors of histone acetylation (C646), deacetylation (Trichostatin A), or the solvent (DMSO) as control. We monitored worker number for six weeks after which we assessed ovary development and sequenced fat body mRNA. Workers survived better in queenless colonies and developed their ovaries after queen removal in control colonies as expected, but not in colonies treated with chemical inhibitors. Both inhibitors affected gene expression, although the inhibition of histone acetylation using C646 influenced the expression of more genes with immunity, fecundity, and longevity functionalities. Interestingly, these C646-treated workers shared many upregulated genes with infertile workers from queenright colonies. We also identified one gene with antioxidant properties commonly downregulated in infertile workers from queenright colonies and both C646 and TSA-treated workers from queenless colonies. Our results indicate that histone acetylation is involved in the molecular regulation of worker reproduction and lifespan, and thus point to an important role of histone modifications in modulating phenotypic plasticity of life history traits in social insects.

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Section: Discussionmentioning

confidence: 99%

Histone acetylation regulates the expression of genes involved in worker reproduction and lifespan in the ant Temnothorax rugatulus

Choppin

Feldmeyer

Foitzik³

2021

Preprint

View full text Add to dashboard Cite

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Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus

2021

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Background In insect societies, queens monopolize reproduction while workers perform tasks such as brood care or foraging. Queen loss leads to ovary development and lifespan extension in workers of many ant species. However, the underlying molecular mechanisms of this phenotypic plasticity remain unclear. Recent studies highlight the importance of epigenetics in regulating plastic traits in social insects. Thus, we investigated the role of histone acetylation in regulating worker reproduction in the ant Temnothorax rugatulus. We removed queens from their colonies to induce worker fecundity, and either fed workers with chemical inhibitors of histone acetylation (C646), deacetylation (TSA), or the solvent (DMSO) as control. We monitored worker number for six weeks after which we assessed ovary development and sequenced fat body mRNA. Results Workers survived better in queenless colonies. They also developed their ovaries after queen removal in control colonies as expected, but not in colonies treated with the chemical inhibitors. Both inhibitors affected gene expression, although the inhibition of histone acetylation using C646 altered the expression of more genes with immunity, fecundity, and longevity functionalities. Interestingly, these C646-treated workers shared many upregulated genes with infertile workers from queenright colonies. We also identified one gene with antioxidant properties commonly downregulated in infertile workers from queenright colonies and both C646 and TSA-treated workers from queenless colonies. Conclusion Our results suggest that histone acetylation is involved in the molecular regulation of worker reproduction, and thus point to an important role of histone modifications in modulating phenotypic plasticity of life history traits in social insects.

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Molecular Description of Proclotting Enzyme: A Power Tool for Insect Biological Control of Aedes aegypti L.1

Cited by 2 publications

References 64 publications

Histone acetylation regulates the expression of genes involved in worker reproduction and lifespan in the ant Temnothorax rugatulus

Histone acetylation regulates the expression of genes involved in worker reproduction and lifespan in the ant Temnothorax rugatulus

Histone acetylation regulates the expression of genes involved in worker reproduction in the ant Temnothorax rugatulus

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