Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach

Kujawa, Joanna; Głodek, Marta; Koter, Izabela; Ośmiałowski, Borys; Knozowska, Katarzyna; Al‐Gharabli, Samer; Dumée, Ludovic F.; Kujawski, Wojciech

doi:10.3390/ma14010201

Cited by 15 publications

(3 citation statements)

References 58 publications

(72 reference statements)

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“…After that, the grafted Al 2 O 3 was centrifuged and washed with dichloromethane, aqueous HCl (pH 2-3), a solution of NaHCO 3 , and distilled water (five times in portions of 10 mL). The grafted powder of Al 2 O 3 was dried over 24 h at 52 • C [40].…”

Section: Procedures For Grafting Of Aluminum Oxidementioning

confidence: 99%

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

et al. 2022

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A strategy for the bioconjugation of the enzyme Candida antarctica lipase B onto titania ceramic membranes with varied pore sizes (15, 50, 150, and 300 kDa) was successfully performed. The relationship between the membrane morphology, i.e.,the pore size of the ceramic support, and bioconjugation performance was considered. Owing to the dimension of the enzyme (~33 kDa), the morphology of the ceramics allowed (50, 150, and 300 kDa) or did not allow (15 kDa) the entrance of the enzyme molecules into the porous structure. Such a strategy made it possible to better understand the changes in the material (morphology) and physicochemical features (wettability, adhesiveness, and surface charge) of the samples, which were systematically examined. The silane functionalization and enzyme immobilization were accomplished via the covalent route. The samples were characterized after each stage of the modification, which was very informative from the material point of view. As a consequence of the modification, significant changes in the contact angle, roughness, adhesion, and zeta potential were observed. For instance, for the 50 kDa membrane, the contact angle increased from 29.1 ± 1.5° for the pristine sample to 72.3 ± 1.5° after silane attachment; subsequently, it was reduced to 57.2 ± 1.5° after the enzyme immobilization. Finally, the contact angle of the bioconjugated membrane used in the enzymatic process rose to 92.9 ± 1.5°. By roughness (Sq) controlling, the following amendments were noticed: for the pristine 50 kDa membrane, Sq = 1.87 ± 0.21 µm; after silanization, Sq = 2.33 ± 0.30 µm; after enzyme immobilization, Sq = 2.74 ± 0.26 µm; and eventually, after the enzymatic process, Sq = 2.37 ± 0.27 µm. The adhesion work of the 50 kDa samples was equal to 136.41 ± 2.20 mN m−1 (pristine membrane), 94.93 ± 2.00 mN m−1 (with silane), 112.24 ± 1.90 mN m−1 (with silane and enzyme), and finally, 69.12 ± 1.40 mN m−1 (after the enzymatic process). The materials and physicochemical features changed substantially, particularly after the application of the membrane in the enzymatic process. Moreover, the impact of ceramic material morphology on the zeta potential value is here presented for the first time. With an increase in the ceramic support cut-off, the amount of immobilized lipase rose, but the specific productivity was higher for membranes possessing smaller pores, owing to the higher grafting density. For the enzymatic process, two modes of accomplishment were selected, i.e., stirred-tank and cross-flow. The latter method was characterized by a much higher effectiveness, with a resulting productivity equal to 99.7 and 60.3 µmol h−1 for the 300 and 15 kD membranes, respectively.

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Section: Procedures For Grafting Of Aluminum Oxidementioning

confidence: 99%

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

et al. 2022

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“…2021 yılında işlevselleştirilmiş alümina malzemesi Candida antarctica lipaz B enzimi immobilizasyonu için kullanıldı. Alümina malzemesi üzerine adsorbsiyon yöntemiyle immobilize edilen lipaz B'nin, spesifik enzim aktivitesi >%97 olarak verilmiştir (28).…”

Section: Aktivite Ve Protein Değerleriunclassified

Hypocrea Jecori̇na’dan Elde Edi̇len Β-Galaktozi̇daz’in Hi̇droksi̇apati̇t Ve Alümi̇na Üzeri̇ne İmmobi̇li̇zasyonu

Türkan¹,

Peksel²

2022

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Hypocrea jecorina QM9414 küf mantarından elde edilen, kısmi olarak saflaştırılan β-galaktozidaz'ın alümina ve hidroksiapatit (HA) üzerine adsorbsiyon yöntemiyle immobilize edilmesi ve bu şekilde hareketsizleştirilmiş olan enzimin serbest enzime göre spesifik aktivite ve protein değerlerinin ölçülerek değerlendirilmesidir. Gereç ve Yöntem: Hypocrea jecorina QM9414 küf mantarından elde edilen, kısmi olarak saflaştırılan β-galaktozidaz enzimi alümina ve hidroksiapatit taşıyıcıları üzerine adsorsiyon yöntemiyle fiziksel olarak bağlandı. Bunun için süzme, yıkama ve kurutma işlemleri yapıldı. Ölçümler için UV-spektrofotometre cihazından yararlanıldı. Bulgular: HA üzerine gerçekleştirilen adsorbsiyon sonucunda immobilize olan enzimin verimi spesifik aktivite birimi (U/mg) açısından %88 bulunurken, protein miktarları (mg/ml) göz önüne alınarak hesaplandığında %18,6 sonucunu göstermiştir. Alümina üzerine gerçekleştirilen adsorbsiyon sonucunda immobilize olan enzimin verimi spesifik aktivite birimi (U/mg) açısından %36,8 bulunurken, protein miktarları (mg/ml) göz önüne alınarak hesaplandığında %22,4 sonucunu göstermiştir. Sonuç: β-galaktozidazın, çok basit bir immobilizasyon protokolü aracılığıyla biyouyumlu HA üzerinde verimli bir şekilde immobilize edilebileceği, tekrarlanan enzimatik hidroliz reaksiyonlarını gerçekleştirmek için umut verici bir strateji sunduğu gösterildi. Hypocrea jecorina QM9414 küf mantarından kısmi olarak saflaştırılan β-galaktozidaz'ın alümina ve hidroksiapatit üzerine adsorbsiyon yöntemiyle immobilize edilmesi literatürde ilk defa yer alacak olması çalışmamızı özgün kılmaktadır.

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“…The obtained results indicated that the specific enzyme activity was much higher for those samples which were functionalized with longer modifiers. The enzyme loading efficiency was found to depend on different factors, such as type of spacer molecules, their chemical composition and the length of the chains, along with enzyme interactions with the surfaces of the carriers [128]. However, these approaches are usually more expensive and complicated when compared with the physical techniques [117].…”

Section: Heterogeneous Enzymatic Reactions For the Remediation Of Phenolic Wastewatersmentioning

confidence: 99%

Applications of Biocatalysts for Sustainable Oxidation of Phenolic Pollutants: A Review

et al. 2021

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Phenol and its derivatives are hazardous, teratogenic and mutagenic, and have gained significant attention in recent years due to their high toxicity even at low concentrations. Phenolic compounds appear in petroleum refinery wastewater from several sources, such as the neutralized spent caustic waste streams, the tank water drain, the desalter effluent and the production unit. Therefore, effective treatments of such wastewaters are crucial. Conventional techniques used to treat these wastewaters pose several drawbacks, such as incomplete or low efficient removal of phenols. Recently, biocatalysts have attracted much attention for the sustainable and effective removal of toxic chemicals like phenols from wastewaters. The advantages of biocatalytic processes over the conventional treatment methods are their ability to operate over a wide range of operating conditions, low consumption of oxidants, simpler process control, and no delays or shock loading effects associated with the start-up/shutdown of the plant. Among different biocatalysts, oxidoreductases (i.e., tyrosinase, laccase and horseradish peroxidase) are known as green catalysts with massive potentialities to sustainably tackle phenolic contaminants of high concerns. Such enzymes mainly catalyze the o-hydroxylation of a broad spectrum of environmentally related contaminants into their corresponding o-diphenols. This review covers the latest advancement regarding the exploitation of these enzymes for sustainable oxidation of phenolic compounds in wastewater, and suggests a way forward.

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Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach

Cited by 15 publications

References 58 publications

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

Hypocrea Jecori̇na’dan Elde Edi̇len Β-Galaktozi̇daz’in Hi̇droksi̇apati̇t Ve Alümi̇na Üzeri̇ne İmmobi̇li̇zasyonu

Applications of Biocatalysts for Sustainable Oxidation of Phenolic Pollutants: A Review

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