Molecular Cytogenetics in Domestic Bovids: A Review

Iannuzzi, Alessandra; Iannuzzi, L.; Parma, P.

doi:10.3390/ani13050944

Cited by 4 publications

(5 citation statements)

References 296 publications

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“…Traditionally, FISH and banding technologies have been central in identifying chromosomes involved in chromosomal anomalies [25]. However, both techniques have required high-quality chromosomal preparations, which are only feasible with fresh blood material.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Robertsonian and Reciprocal Translocations in Cattle through NGS

Iannuzzi,

Pistucci,

Perucatti

et al. 2023

Animals

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This study presents a novel approach that combines next-generation sequencing (NGS) and cytogenetic technologies for identifying chromosomes involved in chromosomal anomalies. This research focuses on a chromosome anomaly discovered in male Alpine Grey cattle, as well as two previously reported cases of reciprocal translocations (rcps), namely rcp(9;11) and rcp(4;7). Abnormal chromosomes from Alpine Grey cattle were microdissected from conventional preparations, and the amplified products were sequenced using NGS. The sequencing reads were then mapped to the reference genome, and the leverage effect was calculated to identify abnormal reads/Mb values. The result revealed the presence of rob(26;29), which was further confirmed through traditional cytogenetic analyses such as Giemsa staining, CBA-banding, RBA-banding, and FISH techniques. Furthermore, the feasibility of this approach on preserved metaphases was demonstrated through analysis of old slides from previously characterized cases. The study highlights the challenges involved in identifying and characterizing chromosomal aberrations in bovine species and offers a potential solution for analyzing historical anomalies when fresh blood material is unavailable. The combination of NGS and cytogenetic techniques provides a cost-effective and reliable approach for characterizing chromosomal anomalies in various species, including those identified before the availability of modern banding technologies and FISH mapping using specific molecular markers.

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Section: Discussionmentioning

confidence: 99%

Characterization of Robertsonian and Reciprocal Translocations in Cattle through NGS

Iannuzzi,

Pistucci,

Perucatti

et al. 2023

Animals

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“…[100,101] qPCR Sperm DNA: TL greater in good quality semen samples than in low quality. [102,103] Bos taurus beef Densitometry Breed differences detected.…”

Section: Telomere Research In Livestockmentioning

confidence: 99%

“…The TL of DNA in the semen of Holstein bulls was suggested as a marker for bull semen quality [102,103]. Bulls with "suitable" semen quality had higher volume and motility, increased viability, and a lower incidence of anomalies than bulls in the "unsuitable" category.…”

Section: Dairy Cattlementioning

confidence: 99%

Telomere Dynamics in Livestock

Zhang,

Baker,

Welsh

et al. 2023

Biology

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Telomeres are repeated sequences of nucleotides at the end of chromosomes. They deteriorate across mitotic divisions of a cell. In Homo sapiens this process of lifetime reduction has been shown to correspond with aspects of organismal aging and exposure to stress or other insults. The early impetus to characterize telomere dynamics in livestock related to the concern that aged donor DNA would result in earlier cell senescence and overall aging in cloned animals. Telomere length investigations in dairy cows included breed effects, estimates of additive genetic control (heritability 0.12 to 0.46), and effects of external stressors on telomere degradation across animal life. Evaluation of telomeres with respect to aging has also been conducted in pigs and horses, and there are fewer reports of telomere biology in beef cattle, sheep, and goats. There were minimal associations of telomere length with animal productivity measures. Most, but not all, work in livestock has documented an inverse relationship between peripheral blood cell telomere length and age; that is, a longer telomere length was associated with younger age. Because livestock longevity affects productivity and profitability, the role of tissue-specific telomere attrition in aging may present alternative improvement strategies for genetic improvement while also providing translational biomedical knowledge.

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“…Vozdova et al identified a specific chromosomal marker for rob(1;29) that allows detection only in bovine spermatozoa 16 . However, this detection technology involves more complex fluorescence in situ hybridization (FISH) analysis compared to classic cytogenetic methods 17 .…”

Section: Introductionmentioning

confidence: 99%

A genomic biomarker for the rapid identification of the rob(1;29) translocation in beef cattle breeds

Iannuzzi,

Demyda-Peyrás,

Pistucci

et al. 2024

Sci Rep

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Robertsonian translocations, specifically rob(1;29) translocation, have reportedly been the most prevalent chromosomal abnormalities in cattle, affecting various breeds and leading to a decrease in fertility and reproductive value. Currently, the identification of rob(1;29) carriers relies on cytogenetic analysis that has limitations in terms of accessibility, cost, and sample requirements. To address these limitations, a novel genomic biomarker was developed in this study for the rapid and precise identification of rob(1;29) carriers. Using q-PCR, a specific copy number variation associated with translocation was targeted, which effectively distinguished between wild-type, homozygous and heterozygous carriers. Crucially, the biomarker can be applied to DNA extracted from various biological matrices, such as semen, embryos, oocytes, milk, saliva, coat, and muscle, and it is compatible with fresh, refrigerated, or frozen samples. Furthermore, this approach offers significant reductions in cost compared to those associated with traditional cytogenetic analysis and provides results within a short turnaround time. The successful development of this genomic biomarker has considerable potential for widespread adoption in screening programs. It facilitates timely identification and management of rob(1;29) carriers while mitigating economic losses and preserving genetic integrity in bovine populations.

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Molecular Cytogenetics in Domestic Bovids: A Review

Cited by 4 publications

References 296 publications

Characterization of Robertsonian and Reciprocal Translocations in Cattle through NGS

Characterization of Robertsonian and Reciprocal Translocations in Cattle through NGS

Telomere Dynamics in Livestock

A genomic biomarker for the rapid identification of the rob(1;29) translocation in beef cattle breeds

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