Molecular cytogenetic study in Octopus (Amphioctopus) areolatus from Japan

Cytogenetic studies in three octopods, Octopus minor, Amphioctopus fangsiao, and Cistopus chinensis from the coast of China

“…Previous studies have shown that the genome size ( C value) of chickens ( Gallusdomesticus ) was 1.25 pg (Tiersch et al 1989, Adachi and Okumura 2012, Adachi et al 2014). Here we determined C values of octopods using this chicken red blood cells as internal standard, and the standard sample was purchased from BD company (DNA QC Particles).…”

“…The previous chromosome analysis of cephalopods had revealed 2n=60 or 92 in most species studied, including O.minor , A.fangsiao , C.chinensis , two sepiids ( Sepiaesculenta and S.lycidas Gray, 1849) and three loliginids ( Heterololigobleekeri Natsukari, 1984, Sepioteuthislessoniana Blainville, 1824 and Photololigoedulis (Hoyle, 1885)) (Gao and Natsukari 1990, Adachi et al 2014, Wang and Zheng 2017), although Nautilusmacromphalus Sowerby, 1849 had 2n=52 chromosomes (Bonnaud et al 2004), and the studies led by Papan and Jazayeri reported the chromosome number of S.arabica Massy, 1916 and S.pharaonis Ehrenberg, 1831 was 2n=48 (Papan et al 2010, Jazayeri et al 2011). However, there are only a few studies on the cephalopod chromosomes in reported publications, and there are no researches on the band type in these species.…”

“…However, there are only a few studies on the cephalopod chromosomes in reported publications, and there are no researches on the band type in these species. Adachi et al (2014) first tried to use fluorescence in situ hybridization on the cephalopod chromosomes and suggested that the telomere sequence of O.areolatus de Haan, 1839–1841 was (TTAGGG)n, but there was a lack of complete and clear metaphases in the report. Due to the restriction of the embryo acquisition, and the number of cephalopod chromosomes up to 60, it is difficult to obtain an ideal metaphase.…”

“…Although the genome sizes of 281 mollusks have been submitted to the Animal Genome Size Database (http://www.genomesize.com. Accessed December 25, 2017) while there just have been 6 species of cephalopod C values that can be obtained from the database: O.bimaculatus (Hinegardner 1974), O.bimaculoides (Albertin et al 2015), O.vulgaris (Packard and Albergoni 1970), Euprymnascolopes (Adachi et al 2014), Loligoplei (Hinegardner 1974) and Loliginidae sp. (Mirsky and Ris 1951).…”

“…As genomic and transcriptomic sequencing is carried out in cephalopods, more and more cephalopod genome sizes have been revealed by complete genome sequencing such as N.pompilius , Architeuthisdux , Hapalochlaenamaculosa , E.scolopes , Idiosepiusparadoxus , L.pealeii , S.officinalis , etc (Yoshida et al 2011, Albertin et al 2012) (Table 1). Besides, Adachi et al (2014) examined the C values of O.ocellatus and O.vulgaris based on flow cytometry. Although there are many methods to detect C values, we choose flow cytometry for the convenience, rapid analysis and relative accuracy of the sample preparation (Gokhman et al 2017).…”