Molecular cytogenetic identification of newly synthetic Triticum kiharae with high resistance to stripe rust

Liu, Xiaojuan; Zhang, Minghu; Liu, Xin; Li, Hongyu; Hao, Ming; Ning, Shunzong; Yuan, Zhongwei; Liu, Dengcai; Wu, Bihua; Chen, Xuejiao; Chen, Wenjie; Zhang, Lianquan

doi:10.1007/s10722-018-0649-y

“…(DD, 2n = 14) [ 1 ]. It combines a set of features of both parental species, as positive – resistance to most diseases and pests, high grain protein and gluten content, the presence of flour substances with antimicrobial properties – defensins [ 2 , 3 ], and negative – low grain yield, hard threshing kernels, very strong gluten [ 1 ]. It is known that T. timopheevii Zhuk.…”

Section: Introductionmentioning

confidence: 99%

Molecular cytological analysis of alien introgressions in common wheat lines derived from the cross of TRITICUM AESTIVUM with T. kiharae

Orlovskaya

¹

,

Dubovets

²

,

Solovey

³

et al. 2020

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Background Triticum kiharae (AtAtGGDD, 2n = 42) is of interest for the improvement of bread wheat as a source of high grain protein and gluten content, as well as resistance to many diseases. The use of T. kiharae for the improvement of T. aestivum L. is complicated by the fact that the homology degree of their genomes is low and this leads to an unbalanced set of chromosomes in the gametes of its first generations and the elimination of some genotypes. The aim of this study was to analyze the nature of alien introgressions and their effect on the cytological stability of hybrids obtained from crossing of bread wheat varieties with T. kiharae. Results Using C-banding, the presence of entire chromosomes of T. kiharae in the karyotypes of hybrid lines (intergenomic substitution 2G/2B), chromosome arms (centric translocation Т2AtS:2AL) and large inserts in the form of terminal translocations involving chromosomes of 1st, 3rd and 5th homoeologous groups of B- and G-genomes were found. Molecular markers revealed short introgression of T. kiharae into the genome of common wheat varieties. The highest introgression frequency was shown for 1A, 1B, 2A, 5B, and 6A chromosomes, while no foreign chromatin was detected in 4A and 4B chromosomes. A high level of cytological stability (a meiotic index of 88.18–93.0%) was noted for the majority of introgression lines. An exception was found for the lines containing the structural reorganization of chromosome 5B, affecting the main genes of chromosome synapsis in terms of their functioning. Conclusions During the stabilization of hybrid karyotypes, the introgression of genetic material from T. kiharae into the genome of T. aestivum occurs in the form of short fragments detectable only by molecular markers and in the form of whole chromosomes (intergenomic substitution) and their large fragments (centric and terminal translocations). The level of cytological stability achieved in F10 by the majority of introgression lines ensures the formation of functional gametes sufficient for the successful reproduction of the obtained hybrids.

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“…Plant height, the tiller number per plant, spike length, and seed setting were observed following Ref. 43 . Data from 10 plants were used to compare trait differences with the t test.…”

Section: Methodsmentioning

confidence: 99%

Development and identification of four new synthetic hexaploid wheat lines with solid stems

Dong

¹

,

Zhang

²

,

Liu

³

et al. 2022

Sci Rep

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Stem solidness is an important agronomic trait for increasing the ability of wheat to resist lodging. In this study, four new synthetic hexaploid wheat with solid stems were developed from natural chromosome doubling of F1 hybrids between a solid-stemmed durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, AABB) and four Aegilops tauschii (2n = 2x = 14, DD) accessions. The solid expression of the second internode at the base of the stem was stable for two synthetic hexalpoid wheat Syn-SAU-117 and Syn-SAU-119 grown in both the greenhouse and field. The lodging resistance of four synthetic solid-stem wheats is stronger than that of CS, and Syn-SAU-116 has the strongest lodging resistance, followed by Syn-SAU-119. The paraffin sections of the second internode showed that four synthetic wheat lines had large outer diameters, well-developed mechanical tissues, large number of vascular bundles, and similar anatomical characteristics with solid-stemmed durum wheat. The chromosomal composition of four synthetic hexaploid wheat was identified by FISH (fluorescence in situ hybridization) using Oligo-pSc119.2-1 and Oligo-pTa535-1. At adult stage, all four synthetic hexaploid wheat showed high resistance to mixed physiological races of stripe rust pathogen (CYR31, CYR32, CYR33, CYR34). These synthetic hexaploid wheat lines provide new materials for the improvement of common wheat.

show abstract

“…Plant height, the tiller number per plant, spike length, and seed setting were observed following Ref. 43 .…”

Section: Agronomic Trait Comparisonsmentioning

confidence: 99%

Development and Identification of Four New Synthetic Hexaploid Wheat Lines with Solid Stems

Dong

¹

,

Zhang

²

,

Liu

³

et al. 2021

Preprint

Self Cite

0

View full text Add to dashboard Cite

Stem solidness is an important agronomic trait for increasing the ability of wheat to resist lodging. In this study, four new synthetic hexaploid wheat with solid stems were developed from natural chromosome doubling of F1 hybrids between a solid-stemmed durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, AABB) and four Aegilops tauschii (2n = 2x = 14, DD) accessions. The solid expression of the second internode at the base of the stem was stable for two synthetic hexalpoid wheat Syn-SAU-117 and Syn-SAU-119 grown in both the greenhouse and field. The lodging resistance of four synthetic solid-stem wheats is stronger than that of CS, and Syn-SAU-116 has the strongest lodging resistance, followed by Syn-SAU-119. The paraffin sections of the second internode showed that four synthetic wheat lines had large outer diameters, well-developed mechanical tissues, large number of vascular bundles, and similar anatomical characteristics with solid-stemmed durum wheat. The chromosomal composition of four synthetic hexaploid wheat was identified by FISH (fluorescence in situ hybridization) using Oligo-pSc119.2-1 and Oligo-pTa535-1. At adult stage, all four synthetic hexaploid wheat showed high resistance to mixed physiological races of stripe rust pathogen (CYR31, CYR32, CYR33, CYR34). These synthetic hexaploid wheat lines provide new materials for the improvement of common wheat.

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Molecular cytogenetic identification of newly synthetic Triticum kiharae with high resistance to stripe rust

Cited by 9 publications

References 16 publications

Molecular cytological analysis of alien introgressions in common wheat lines derived from the cross of TRITICUM AESTIVUM with T. kiharae

Molecular cytological analysis of alien introgressions in common wheat lines derived from the cross of TRITICUM AESTIVUM with T. kiharae

Development and identification of four new synthetic hexaploid wheat lines with solid stems

Development and Identification of Four New Synthetic Hexaploid Wheat Lines with Solid Stems

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