Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance

Hu, Lijun; Guang-rong, LI; Zeng, Zixian; Chang, Zhijian; Liu, Cheng; Zhou, Jianping; Yang, Zujun

doi:10.1007/s10681-010-0216-x

Cited by 42 publications

(35 citation statements)

References 43 publications

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“…Further, PCR markers based on conserved genetic regions show high levels of collinearity among the cereal genomes, such as rice, Brachypodium, and wheat [Ishikawa et al, 2007]. We previously used PLUG markers to identify chromosomal introgressions from Thinopyrum intermedium [Hu et al, 2011a], T. ponticum [Hu et al, 2011b], and Secale africanum [Lei et al, 2012] and its subsequent assignment to the corresponding linkage group. In the present study, the wheat chromosome group-2 PLUG markers detected polymorphic fragments specific to the D. breviaristatum chromosome in D11-5 ( fig.…”

Section: Discussionmentioning

confidence: 99%

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A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

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The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrumbreviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V^b (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V^b were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V^b of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.

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Section: Discussionmentioning

confidence: 99%

“…The cycling parameters were 94 ° C for 3 min for denaturation, followed by 35 cycles at 94 ° C for 1 min, 55 ° C for 1 min, 72 ° C for 2 min, and a final extension at 72 ° C for 10 min. The amplified products were separated by 8% PAGE as described by Hu et al [2011a].…”

Section: Molecular Marker Analysismentioning

confidence: 99%

A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

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“…We previously used PLUG markers [Ishikawa et al, 2009] to identify chromosomal introgressions from Thinopyrum intermedium (Host) Barkworth & D.R. Dewey [Hu et al, 2011a], Th. ponticum [Hu et al, 2011b], Dasypyrum [Li et al, 2014], and Secale africanum Stapf.…”

Section: Discussionmentioning

confidence: 99%

Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

Wang

Yü

et al. 2018

Cytogenet Genome Res

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Dasypyrum breviaristatum (genome V^bV^b) contains potentially important traits for commercial wheat production. Chromosome 2V^b of D. breviaristatum carries several desirable agronomic characters, including long spike length as well as enhanced resistance to stripe rust, which are expressed in a common wheat background. In this study, wheat-D. breviaristatum 2V^b deletion lines were produced and identified by fluorescence in situ hybridization (FISH), and 74 molecular markers specific to D. breviaristatum chromosome 2V^b were physically localized in 4 distinct chromosomal regions. New wheat-D. breviaristatum 2V^b translocation lines were also characterized by FISH. The breakpoint of the translocation T3AS.3AL-2V^bS was determined by physically mapped molecular markers. Field evaluation revealed that genes affecting plant height and spike length are located on fraction length (FL) 0.65-1.00 of 2V^bS, while the stripe rust resistance gene(s) are located on FL 0.40-1.00 of D. breviaristatum chromosome 2V^bL. The newly characterized wheat-Dasypyrum chromosomal introgressions are of potential value for the improvement of the yield and disease resistance of wheat.

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“…intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation lines, which contained novel agronomically important genes (Hu et al 2011;Li et al 2013Li et al , 2015a. With the aim to precisely trace the 1St#2 chromosomes by marker assisted selection in wheat breeding programs for improving grain quality, the SLAF-seq technology was applied to wheat-Th.…”

Section: Introductionmentioning

confidence: 99%

New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

Guang-rong

Wang

Lang

et al. 2016

Planta

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New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.

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Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance

Abstract: A new wheat-Thinopyrum substitution line AS1677, developed from a cross between wheat line ML-13 and wheat-Thinopyrum intermedium ssp. trichophorum partial amphiploid TE-3, was characterized by fluorescence in situ hybridization (FISH)

Cited by 42 publications

References 43 publications

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

Characterization of New Wheat-<b><i>Dasypyrum breviaristatum</i></b> Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

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