Molecular cytogenetic identification of a wheat‐<i>Aegilops geniculata</i> Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress

Landjeva, S.; Kocheva, Konstantina; Karceva, Tania; Sepsi, Adél; Molnár, István; Schneider, A.; Ganeva, G.; Georgiev, G. I.; Molnár-Láng, Márta

doi:10.1111/j.1439-0523.2011.01918.x

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…1a). The same preparations were then subjected to FISH using the four probes pSc119.2, Afa family repeats, pTa71 and (CTT) 5 following suggestions made elsewhere in the literature [10–12, 23, 24]. Most of the pSc119.2 sites were found to lie near the telomeres, while those recognized by the Afa family repeats probe were limited to chromosomes 2M g , 3M g and 7M g (Fig.…”

Section: Resultsmentioning

confidence: 92%

The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat

Zheng

Ning

et al. 2019

Mol Cytogenet

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Background The annual allotetraploid species Aegilops geniculata harbors a number of traits relevant for wheat improvement. An effective cytogenetic method has yet to be developed to distinguish between each of its 14 chromosomes. Results A fluorescence in situ hybridization (FISH) approach was adopted to describe the karyotype of Ae. geniculata. Each of its 14 chromosomes was unequivocally recognized using a cocktail of three probes, namely pTa-713, (AAC) 5 and pTa71. FISH karyotyping was then used to detect and characterize selections from an Ae. geniculata × bread wheat wide cross of a chromosome 1M g disomic addition line and three 4M g (4B) substitution lines. The identity of the addition line was confirmed by the presence of Glu-M1 , detected both using an SDS-PAGE separation of endosperm proteins and by applying a PCR assay directed at the Glu-M1 locus. The status of the substitution lines was validated by genotyping using a wheat single nucleotide polymorphism chip. Conclusion FISH karyotyping based on pTa-713, (AAC) 5 and pTa71 will be useful for determining the contribution of Ae. geniculata to derivatives of an Ae. geniculata × wheat wide cross. SNP chip-based genotyping is effective for confirming the status of whole chromosome wheat/alien substitution lines. Electronic supplementary material The online version of this article (10.1186/s13039-019-0428-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 92%

The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat

Zheng

Ning

et al. 2019

Mol Cytogenet

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“…While 6E b (6D) lines were generally as tall as Roushan in the field, line 2E b (2B) showed ca.15 cm decreased plant height than Roushan and was as tall as the Tritipyrum parent. The spike of the 2E b (2B) substitution line had a lax base, compact top, and short awns, which are characteristic of the 2M g chromosome addition or substitution with 2A in a Chinese Spring wheat background (Friebe et al 1999, Landjeva et al 2012. DS2E b (2B) headed about 12 d later than Roushan .…”

Section: Discussionmentioning

confidence: 99%

Genome Structure and Salt Stress Response of Some Segregated Lines from Wheat and Tritipyrum Crosses

2013

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SummaryWe evaluated the genome structure and salt stress response of BC 1 F 6 lines derived from a cross between an Iranian salt tolerant bread wheat cultivar Roushan (2n=6x=42, AABBDD) and a synthetic allohexaploid Tritipyrum (2n=6x=42, AABBE b E b ). Sixteen lines (L1 to L16) and their parents were studied for their chromosome composition, K + : Na + ratio, and relative dry mass (RDM) by growing them in nutrient solution containing 0 (control) or 100 mM NaCl (treatment). Among the studied lines, eight wheat Thinopyrum bessarabicum (E b genome) disomic substitution lines were identified using PCR-based Landmark Unique Gene (PLUG) markers and cytogenetic analysis. Ten (37%) of 27 PLUG markers used were informative for E b chromosomes 1, 2, 3, 4, and 6 in a wheat background. PLUG markers also revealed that one line, L9, was a disomic substitution line DS2E b (2B), and that seven lines, L3, L4, L7, L8, L14, L15, and L16, were DS6E b (6D). There were large differences in morphological traits and salinity tolerance even among DS6E b (6D) substitutions or untranslocated lines, due perhaps to unidentified rearrangements or small introgressions.Key words Disomic substitution line, EST-based marker, Thinopyrum bessarabicum, Wheat. Domestication and modern plant breeding have narrowed the genetic base of bread wheat (Triticum aestivum; 2n=6x=42, AABBDD), which could restrict future crop improvement (Reif et al. 2005). Plants are constantly exposed to environmental stresses that limit their growth and productivity. Seven percent of the land in Iran contain salt either in the irrigation water or in soil (Zohary 1973). Wheat (Triticum aestivum L.) is the most important and widely adapted cereal in this country, and breeding for salt tolerance is one of the most efficient ways to increase its yield.To increase the salt tolerance of wheat, most of the attention has been focused on identifying genetic sources with low rates of transport of Na + to leaves, high selectivity for K + over Na + (i.e. Na + exclusion ), and higher performance . Wide hybridization of some halophytic wild species in the Triticeae with wheat has been proposed as a strategy to increase salt tolerance beyond the existing cultivated wheat gene pool (Colmer et al. 2006). Amongst the wild relatives, tall wheatgrass species, e.g. Thinopyrum bessarabicum (Save ex Rayss) A Löve (2n=2x=14,and Lophopyrum elongatum (Host) A. Löve (2n=2x=14, EE), are important sources of genes for agronomically desirable traits, particularly salt tolerance (Dvorak and Ross 1986). Wheat Th. bessarabicum amphiploids (Tritipyrums) have been produced and one such amphiploid was shown to survive in 250 mM NaCl, equivalent to halfstrength sea water (Forster et al. 1988). The salt tolerance potential of Tritipyrum and some of the disomic addition and substitution lines derived from a cross between wheat and Tritipyrum have been evaluated, and the positive contribution of 5E b addition lines has been reported (King et al. 1997a).Cytogenetic methods and molecular markers have been used in s...

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Molecular cytogenetic identification of a wheat‐Aegilops geniculata Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress

Cited by 2 publications

References 32 publications

The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat

The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat

Genome Structure and Salt Stress Response of Some Segregated Lines from Wheat and Tritipyrum Crosses

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