Molecular Cytogenetic Definition of the Chicken Genome: The First Complete Avian Karyotype

Masabanda, Julio S.; Burt, David W.; O’Brien, Patricia C.; Vignal, Alain; Fillon, Valérie; Walsh, Philippa S; Cox, Helen; Tempest, Helen G.; Smith, Jacqueline; Habermann, Felix A.; Schmid, Michael; Matsuda, Yoichi; Ferguson‐Smith, Malcolm A.; Crooijmans, R.P.M.A.; Groenen, Martien A. M.; Griffin, Darren K.

doi:10.1534/genetics.166.3.1367

Cited by 124 publications

(133 citation statements)

References 43 publications

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“…Chicken (Gallus gallus, GGA) chromosome-specific DNA probes of chromosome 1-9 and Z (GGA1-9 and GGAZ) were used for comparative chromosome painting , Masabanda et al 2004. Each probe was amplified by DOP-PCR following Carter et al (1992) and hybridized to the metaphase spreads.…”

Section: Dna Probesmentioning

confidence: 99%

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The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

et al. 2007

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Palaeognathous birds (Struthioniformes and Tinamiformes) have morphologically conserved karyotypes and less differentiated ZW sex chromosomes. To delineate interspecific chromosome orthologies in palaeognathous birds we conducted comparative chromosome painting with chicken (Gallus gallus, GGA) chromosome 1-9 and Z chromosome paints (GGA1-9 and GGAZ) for emu, double-wattled cassowary, ostrich, greater rhea, lesser rhea and elegant crested tinamou. All six species showed the same painting patterns: each probe was hybridized to a single pair of chromosomes with the exception that the GGA4 was hybridized to the fourth largest chromosome and a single pair of microchromosomes. The GGAZ was also hybridized to the entire region of the W chromosome, indicating that extensive homology remains between the Z and W chromosomes on the molecular level. Comparative FISH mapping of four Z-and/or W-linked markers, the ACO1/IREBP, ZOV3 and CHD1 genes and the EE0.6 sequence, revealed the presence of a small deletion in the proximal region of the long arm of the W chromosome in greater rhea and lesser rhea. These results suggest that the karyotypes and sex chromosomes of palaeognathous birds are highly conserved not only morphologically, but also at the molecular level; moreover, palaeognathous birds appear to retain the ancestral lineage of avian karyotypes.2

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Section: Dna Probesmentioning

confidence: 99%

“…Karyotypes can be subdivided into large macrochromosomes including ZW-type sex chromosomes (7-10 pairs) and a large number of almost indistinguishable microchromosomes (Takagi & Sasaki 1974, Belterman & de Boer 1984 or into groups A-D (Masabanda et al 2004). …”

Section: Introductionmentioning

confidence: 99%

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

et al. 2007

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“…and information it provides for future research (Antin and Konieczka 2005). Together with chromosome paints, BAC clones (BPRC) have been used to define cytogenetically all chicken chromosomes (Masabanda et al 2004). Because of the nearly identical sizes of the microchromosomes in mitotic chromosome spreads, this was not previously feasible.…”

Section: New Tools For Genome Analysismentioning

confidence: 99%

Chicken genome: Current status and future opportunities

Burt¹

2005

Genome Res.

144

101

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The chicken genome sequence is important for several reasons. First, the chicken shared a common ancestor with mammals ∼310 million years ago (Mya) at a phylogenetic distance not previously covered by other genome sequences. It therefore fills a gap in our knowledge and understanding of the evolution and conservation of genes, regulatory sequences, genomes, and karyotypes. The chicken is also a major source of protein in the world, with billions of birds used in meat and egg production each year. It is the first livestock species to be sequenced and so leads the way for others. The sequence and the 2.8 million genetic polymorphisms defined in a parallel project are expected to benefit agriculture and cast new light on animal domestication. Also, as the first bird to be sequenced, it is a model for the 9600 avian species thought to exist today. Many of the features of the chicken genome and its biology make it an ideal organism for studies in development and evolution, along with applications in agriculture and medicine.

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“…In this study, 10 duck loci were assigned to duck macrochromosomes, 10 loci to small macrochromosomes, and 4 loci to microchromosomes using chicken BAC clones. Much research has shown strong chromosome homology between macrochromosomes in different bird species (Masabanda et al 2004). In our research, 19 orthologous loci (CAUD023, CAUD069, AMU182, APH12, CAUD049, CAUD138, CAUD091, CAUD128, CAUD109, AMU6, CAUD111, CAUD022, APH20, CAUD038, AMU52, AMU174, CAUD044, CAUD080, and CAUD123) were assigned to the same macrochromosome or small macrochromosome in duck and chicken, respectively, 1 orthologous locus (CAUD021) was located on duck chromosome 4, but uncertain in the chicken genome.…”

Section: Tablementioning

confidence: 99%

A Genetic and Cytogenetic Map for the Duck (Anas platyrhynchos)

et al. 2006

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A genetic linkage map for the duck (Anas platyrhynchos) was developed within a cross between two extreme Peking duck lines by linkage analysis of 155 polymorphic microsatellite markers, including 84 novel markers reported in this study. A total of 115 microsatellite markers were placed into 19 linkage groups. The sexaveraged map spans 1353.3 cM, with an average interval distance of 15.04 cM. The male map covers 1415 cM, whereas the female map covers only 1387.6 cM. All of the flanking sequences of the 155 polymorphic loci-44 monomorphic loci and a further 41 reported microsatellite loci for duck-were blasted against the chicken genomic sequence, and corresponding orthologs were found for 49. To integrate the genetic and cytogenetic map of the duck genome, 28 BAC clones were screened from a chicken BAC library using the specific PCR primers and localized to duck chromosomes by FISH, respectively. Of 28 BAC clones, 24 were detected definitely on duck chromosomes. Thus, 11 of 19 linkage groups were localized to 10 duck chromosomes. This genetic and cytogenetic map will be helpful for the mapping QTL in duck for breeding applications and for conducting genomic comparisons between chicken and duck.

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Molecular Cytogenetic Definition of the Chicken Genome: The First Complete Avian Karyotype

Cited by 124 publications

References 43 publications

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

Chicken genome: Current status and future opportunities

A Genetic and Cytogenetic Map for the Duck (Anas platyrhynchos)

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