Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, <i>Dibothriocephalus latus</i> (Diphyllobothriidea)

Orosová, Martina; Marková, Anna; Provazníková, Irena; Oros, Mikuláš; Radačovská, Alžbeta; Čadková, Zuzana; Marec, František

doi:10.1017/s0031182021000408

Cited by 7 publications

(11 citation statements)

References 52 publications

(63 reference statements)

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“…The mean and standard deviation were calculated using Microsoft Excel. Staining with silver nitrate (AgNO 3 ) was performed according to the standard protocol commonly used for Cestoda (Orosová et al ., 2021). Fluorescent YOYO-1 staining to visualize the nucleoli was performed according to the previously described procedure (Orosová et al ., 2010 b ).…”

Section: Methodsmentioning

confidence: 99%

“…The collected data on tapeworms indicated the prevalence of chromosome complements with only one locus of rDNA (NOR), which was always interstitially located on a small metacentric chromosome and associated with C-DAPI + heterochromatin (Orosová et al, 2010b(Orosová et al, , 2019(Orosová et al, , 2021Orosová and Oros, 2012). However, in C. fennica, two clusters of different sizes were observed on two chromosome pairs, and in triploid A. huronensis, two clusters were found on one trivalent (Kráľová-Hromadová et al, 2010;Orosová et al, 2010a;Špakulová et al, 2019).…”

Section: Arrangement Of Rdna In Chromosomes Of Caryophyllidean Tapewormsmentioning

confidence: 99%

“…To date, the first cytogenetic review of the Cestoda, including the monozoic order Caryophyllidea (Špakulová et al ., 2011), has been supplemented by several later studies (Orosová and Oros, 2012; Bombarová and Špakulová, 2015; Orosová et al ., 2019, 2021; Špakulová et al ., 2019). However, the karyotypes of taxonomically related species studied within each family showed very inconsistent characteristics.…”

Section: Introductionmentioning

confidence: 99%

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New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest

et al. 2022

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Caryophyllideans are intestinal parasites of freshwater fishes, occupying a basal position among the ‘true’ tapeworms. We performed detailed cytogenetic analyses of the well-known caryophyllidean species Caryophyllaeus laticeps. For comparison, we also examined for the first time the chromosomes of Paracaryophyllaeus gotoi, a specific parasite of loaches in China. Both species showed a diploid chromosome number of 2n = 20, n = 10m. Chromomycin A3 (CMA3)/diamidino-2-phenylindole (DAPI) staining performed for the first time in the class Cestoda revealed CMA3+/DAPI− bands in the pericentromeric regions of the short arms of chromosome pair no. 7 in the karyotype of C. laticeps. Fluorescence in situ hybridization with the 18S rDNA probe confirmed the presence of a single cluster of major rDNA near the centromere on a pair of small chromosomes in both species. These findings support the hypothesis that the ancestral state in the family Caryophyllaeidae is a single interstitial cluster of major rDNA genes and thus one nucleolar organizer region per haploid genome. Our results, which we presented together with literature data plotted on a phylogenetic tree, show stability of caryophyllidean karyotypes at the genus level, but showed differences between genera without a clear phylogenetic signal. The data allowed us to at least formulate a hypothesis about the ancestral haploid chromosome number of n = 10 for the family Caryophyllaeidae and possibly for the sister family Capingentidae. In addition, we compared two populations of C. laticeps from water bodies with different levels of polychlorinated biphenyl contamination, showing a slightly increased incidence of chromosomal abnormalities at the contaminated site.

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Section: Methodsmentioning

confidence: 99%

Section: Arrangement Of Rdna In Chromosomes Of Caryophyllidean Tapewormsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest

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“…The ongoing circulation of D. latus in the natural environment of subalpine lakes has offered biological material for molecular and genetic studies of the parasite (Nicoulaud et al, 2005;Yera et al, 2006Yera et al, , 2008Wicht et al, 2007Wicht et al, , 2010aWicht et al, , 2010bBazsalovicsová et al, 2018). A recent in-depth karyological analysis of 2 D. latus specimens from European perch from Iseo lake (northern Italy) found a very atypical chromosomal structure (Orosová et al, 2021). Both tapeworms contained majority of the cells with the triploid number of chromosomes (3n = 27, 46.7%), but 5.9% of cells displayed diploid (2n = 18) chromosomal set.…”

Section: Introductionmentioning

confidence: 99%

“…The course of meiosis was aberrant and indicated an absence of fully developed spermatic cells and mature functional spermatozoa. Parthenogenetic reproduction was concluded to be the most probable method of reproduction of triploid D. latus from Iseo lake (Orosová et al , 2021).…”

Section: Introductionmentioning

confidence: 99%

Unique genetic structure of the human tapeworm Dibothriocephalus latus from the Alpine lakes region – a successful adaptation?

et al. 2022

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Dibothriocephalus latus is the most frequent causative agent of fish-borne zoonosis (diphyllobothriosis) in Europe, where it is currently circulating mainly in the Alpine lakes region (ALR) and Russia. Three mitochondrial genes (cox1, cob and nad3) and 6 microsatellite loci were analysed to determine how is the recently detected triploidy/parthenogenesis in tapeworms from ALR displayed at the DNA level. A geographically distant population from the Krasnoyarsk Reservoir in Russia (RU-KR) was analysed as a comparative population. One or 2 alleles of each microsatellite locus was detected in plerocercoids from RU-KR, corresponding to the microsatellite pattern of a diploid organism. In contrast, 1–3 alleles were observed in tapeworms from ALR, in accordance with their triploidy. The high diversity of mitochondrial haplotypes in D. latus from RU-KR implied an original and relatively stable population, but the identical structure of mitochondrial genes of tapeworms from ALR was probably a consequence of a bottleneck typical of introduced populations. These results indicated that the diploid/sexually reproducing population from RU-KR was ancestral, located within the centre of the distribution of the species, and the triploid/parthenogenetically reproducing subalpine population was at the margin of the distribution. The current study revealed the allelic structure of the microsatellite loci in the triploid tapeworm for the first time.

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Mitochondrial and microsatellite data show close genetic relationships between Dibothriocephalus latus from South America (Argentina) and Europe (the Alpine lakes region)

2022

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Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)

Abstract: Abstract

Cited by 7 publications

References 52 publications

New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest

New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest

Unique genetic structure of the human tapeworm Dibothriocephalus latus from the Alpine lakes region – a successful adaptation?

Mitochondrial and microsatellite data show close genetic relationships between Dibothriocephalus latus from South America (Argentina) and Europe (the Alpine lakes region)

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