Abstract:Monovalent salt shifts the equilibrium toward the hairpin conformation by slowing the opening transition. In contrast, molecular crowding by PEG accelerates both the opening and closing transitions.The experiments examined the conformational changes of trinucleotide repeat-containing DNA hairpins that occur through formation and melting of intramolecular base pairs. The hairpins can exist in two forms: an open (unpaired) state and a closed (base paired) state ( Figure 1). Single-molecule fluorescence resonance… Show more
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