Molecular control of vascular development in the zebrafish

Holden, B J; Bratt, D; Chico, Timothy J. A.

doi:10.1002/bdrc.20204

Cited by 8 publications

(4 citation statements)

References 63 publications

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“…Stimulation of VEGF-A signaling has been shown to promote the growth and migration of endothelial cells in ISV development [49]. Further interaction with notch signaling is also required for perfecting proper ISV patterning [50].…”

Section: Discussionmentioning

confidence: 99%

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

McCollum

Conde‐Vancells

Hans

et al. 2017

Reproductive Toxicology

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To identify vascular disruptor compounds (VDCs), this study utilized an in vivo zebrafish embryo vascular model in conjunction with a mouse endothelial cell model to screen a subset of the U.S. Environmental Protection Agency (EPA) ToxCast Phase I chemical inventory. In zebrafish, 161 compounds were screened and 34 were identified by visual inspection as VDCs, of which 28 were confirmed as VDCs by quantitative image analysis. Testing of the zebrafish VDCs for their capacity to inhibit endothelial tube formation in the murine yolk-sac-derived endothelial cell line C166 identified 22 compounds that both disrupted zebrafish vascular development and murine endothelial in vitro tubulogenesis. Putative molecular targets for the VDCs were predicted using EPA’s Toxicological Prioritization Index tool and a VDC signature based on a proposed adverse outcome pathway for developmental vascular toxicity. In conclusion, our screening approach identified 22 novel VDCs, some of which were active at nanomolar concentrations

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Section: Discussionmentioning

confidence: 99%

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

McCollum

Conde‐Vancells

Hans

et al. 2017

Reproductive Toxicology

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“…In our studies, the importance of Pou3f2 in endothelial development in vivo was assessed using the zebrafish model. The availability of transgenics expressing endothelial-specific fluorescent reporters, for example Tg( fli1:EGFP ) y1 , combined with the transparency of the embryo, facilitate visualization of vascular development and blood flow in real time (Baldessari and Mione, 2008; Ellertsdóttir et al, 2010; Holden et al, 2011; Kamei et al, 2010). …”

Section: Resultsmentioning

confidence: 99%

Discovery of novel determinants of endothelial lineage using chimeric heterokaryons

Wong

Matrone

Tian

et al. 2017

eLife

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We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq, it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern of gene expression in the ESCs of the heterokaryons that recapitulated ontogeny, with early mesodermal factors being expressed before mature endothelial genes. A set of transcriptional factors not known to be involved in endothelial development was upregulated, one of which was POU class 3 homeobox 2 (Pou3f2). We confirmed its importance in differentiation to endothelial lineage via loss- and gain-of-function (LOF and GOF). Its role in vascular development was validated in zebrafish embryos using morpholino oligonucleotides. These studies provide a systematic and mechanistic approach for identifying key regulators in directed differentiation of pluripotent stem cells to somatic cell lineages.DOI: http://dx.doi.org/10.7554/eLife.23588.001

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“…Subsequently, and by specific segmentation and rotation processes, the cardiac tube produces the different well-defined heart chambers: a venous sinus, an atrium, a ventricle and the arterial bulb. These four chambers are arranged in series and are separated by narrowing heart valves that ensure unidirectional blood flow [10,12,15,[25][26][27]. At the same time, hemangioblasts differ from the mesoderm, which produces angioblasts and endothelial cells.…”

Section: Cardiovascular Development In Zebrafishmentioning

confidence: 99%

Zebrafish Heart as a Model to Elucidate the Mechanisms of Sudden Cardiac Death

Vargas¹

2020

JICOA

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The heart of zebrafish has been used as a simple, low-cost model to study development, structure and function of the heart from early to late stages of life. Also, it has been established as a model to study different cardiac pathologies generated through different methods. Cardiac pathologies include from functional disorders such as arrhythmia, to structural disorders such as hypertrophic heart disease; in many of them, genetic and molecular aspects have been associated. Noteworthy, some of these genetic and molecular factors have been invoked as causes of sudden cardiac death in humans. This adverse outcome is common in many cardiovascular pathologies and can occur at any age. Unfortunately, etiology and physiopathology of sudden cardiac death are unclear, so extensive research in this area is required. This mini-review highlight three related points: First, the relevance of sudden cardiac death in humans. Second, advances in knowledge of the development, function and pathologies in the zebrafish heart model. Finally, the possibility of using the zebrafish model for the study of sudden cardiac. For this review, a literature search was performed using the PubMed database and the search engine Google Scholar: the words sudden cardiac death, zebrafish, arrhythmias, cardiomyopathies were combined for this search.

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Molecular control of vascular development in the zebrafish

Abstract: The zebrafish is emerging as a novel model for the study of embryonic vascular development. In this review we summarize the advantages of this intriguing experimental system and the advances in our understanding of the molecular control of vascular development it has allowed.

Cited by 8 publications

References 63 publications

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

Discovery of novel determinants of endothelial lineage using chimeric heterokaryons

Zebrafish Heart as a Model to Elucidate the Mechanisms of Sudden Cardiac Death

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