Molecular control of microsporogenesis in Arabidopsis

Chang, Fang Rong; Wang, Yingxiang; Wang, Shuangshuang; Mā, Hong

doi:10.1016/j.pbi.2010.11.001

Cited by 89 publications

(82 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the transcription factor genes DYT1, MYB35/TDF, AMS, MS188/MYB103/MYB80, and MS1 are required for normal anther development in a DYT1-MYB35/ TDF1-AMS-MS188/MYB103/MYB80-MS1 regulatory pathway (Ge et al, 2010;Chang et al, 2011;Zhu et al, 2011) and AMS was reported to act as a master regulator coordinating pollen wall development , we further examined the expression levels of genes encoding these five transcription factors in DN-CLE19 and CLE19-OX anthers using quantitative realtime PCR analyses. The expression levels of AMS, MS188/MYB103/MYB80, and MS1 showed over 2-fold increases in DN-CLE19 but over 2-fold decreases in CLE19-OX anthers (Fig.…”

Section: The Expression Of Ams and Downstream Transcription Factor Gementioning

confidence: 99%

“…Anther development is precisely regulated temporally by transcriptional networks, receptor-like protein kinase-mediated signal pathways, and other pathways (Ma, 2005;Ge et al, 2010;Chang et al, 2011). The basic helix-loop-helix (bHLH) transcriptional factor DYSFUNCTIONAL TAPETUM1 (DYT1) is among the earliest acting male-specific regulators and is required for the normal development of tapetum by regulating the normal expression of more than 1,000 anther genes, and dyt1 mutants form abnormal prematurely vacuolated tapetal cells and lack mature pollen (Zhang et al, 2006;Feng et al, 2012;Gu et al, 2014;Zhu et al, 2015).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Wang

Song

et al. 2017

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding the possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN) and overexpression (OX) transgenic plants of the CLE19 gene, exhibited significantly reduced anther size and pollen grain number and abnormal pollen wall formation in Arabidopsis (Arabidopsis thaliana). Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface, but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae. With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated and swollen aborted microspores (ams)-like tapetal cells, lacked lipidic tapetosomes and elaioplasts, and had abnormal pollen primexine without obvious accumulation of sporopollenin precursors. Moreover, CLE19 is important for the normal expression of more than 1,000 genes, including the transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat and pollen exine formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(2/2) plants exhibited the ams anther phenotype and ams(+/2) partially suppressed the DN-CLE19 transgeneinduced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.Pollen grains are generated in the male reproductive organ anther of flowering plants and are essential for plant fertility. In the model plant Arabidopsis (Arabidopsis thaliana), pollen grains are ellipsoidal, and the pollen surface is covered by a reticulate exine. In contrast, rice (Oryza sativa) pollen grains are globular and have a smooth surface without the reticulate structure. A well-organized pollen wall structure is essential for the physical and chemical stability of the mature pollen by providing protection for pollen grains from environmental stresses, such as desiccation and microbial attacks (Scott et al., 2004). The pollen wall is composed mainly of three layers: the intine layer, the exine layer, and the pollen coat (Zinkl et al., 1999;Edlund et al., 2004;Blackmore et al., 2007). The intine is the innermost layer of the pollen wall immediately adjacent to the plasma membrane of the pollen vegetative cell and is composed mainly of pectin, cellulose, hemicellulose, hydrolytic enzymes, and hydrophobic proteins (Scott et al., 2004). Both the exine layer and the pollen coat layer are basically of a lipidic nature. The exine is largely formed from acyl lipid and phenylpropanoid precursors, which together form the mixed stable biopolymer known as sporopollenin (Gui...

show abstract

Section: The Expression Of Ams and Downstream Transcription Factor Gementioning

confidence: 99%

mentioning

confidence: 99%

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Wang

Song

et al. 2017

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Extensive studies have shown that the finely tuned process of male gametophytic development requires contributions by and collaborations from the surrounding sporophytic cells (Wilson and Zhang, 2009;Parish and Li, 2010;Chang et al, 2011). Microsporocytes in anther locules are surrounded by four somatic cell layers, the epidermis, endothecium, middle layer, and tapetum, during stages 1 to 5 in Arabidopsis thaliana (Sanders et al, 1999) or stages 1 to 6 in rice (Oryza sativa) .…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Production of Reactive Oxygen Species by NADPH Oxidase Is Critical for Tapetal Programmed Cell Death and Pollen Development in Arabidopsis

2014

View full text Add to dashboard Cite

Male sterility in angiosperms has wide applications in agriculture, particularly in hybrid crop breeding and gene flow control. Microspores develop adjacent to the tapetum, a layer of cells that provides nutrients for pollen development and materials for pollen wall formation. Proper pollen development requires programmed cell death (PCD) of the tapetum, which requires transcriptional cascades and proteolytic enzymes. Reactive oxygen species (ROS) also affect tapetal PCD, and failures in ROS scavenging cause male sterility. However, many aspects of tapetal PCD remain unclear, including what sources generate ROS, whether ROS production has a temporal pattern, and how the ROS-producing system interacts with the tapetal transcriptional network. We report here that stage-specific expression of NADPH oxidases in the Arabidopsis thaliana tapetum contributes to a temporal peak of ROS production. Genetic interference with the temporal ROS pattern, by manipulating RESPIRATORY-BURST OXIDASE HOMOLOG (RBOH) genes, affected the timing of tapetal PCD and resulted in aborted male gametophytes. We further show that the tapetal transcriptional network regulates RBOH expression, indicating that the temporal pattern of ROS production intimately connects to other signaling pathways regulated by the tapetal transcriptional network to ensure the proper timing of tapetal PCD.

show abstract

“…The regulation of stamen development has been intensively investigated because, in addition the key role that stamens play in completion of the plant life cycle, male sterility caused by defects in stamen development can be exploited for the generation of crop plants that exhibit heterosis (Goldberg et al, 1993;Ma, 2005;Chang et al, 2011;Kelliher et al, 2014;Walbot and Egger, 2016).…”

mentioning

confidence: 99%

Evidence of Human-to-Swine Transmission of the Pandemic (H1N1) 2009 Influenza Virus in South Korea

et al. 2010

View full text Add to dashboard Cite

This study aimed at elucidating regulatory components behind floral organ identity determination and tissue development. It remains unclear how organ identity proteins facilitate development of organ primordia into tissues with a determined identity, even though it has long been accepted that floral organ identity is genetically determined by interaction of identity genes according to the ABC model. Using the chromatin immunoprecipitation sequencing technique, we identified OsTGA10, encoding a bZIP transcription factor, as a target of the MADS box protein OsMADS8, which is annotated as an E-class organ identity protein. We characterized the function of OsTGA10 using genetic and molecular analyses. OsTGA10 was preferentially expressed during stamen development, and mutation of OsTGA10 resulted in male sterility. OsTGA10 was required for tapetum development and functioned by interacting with known tapetum genes. In addition, in ostga10 stamens, the hallmark cell wall thickening of the endothecium was defective. Our findings suggest that OsTGA10 plays a mediator role between organ identity determination and tapetum development in rice stamen development, between tapetum development and microspore development, and between various regulatory components required for tapetum development. Furthermore, the defective endothecium in ostga10 implies that cell wall thickening of endothecium is dependent on tapetum development.The ABC model for the genetic control of floral organ identity determination is the most influential theory in plant developmental biology in the last three decades (Coen and Meyerowitz, 1991). This model proposes that transcription factors encoded by three classes of genes, namely A, B, and C, determine organ identities, acting either alone or in conjunction with one another, for sepals, petals, stamens, and carpels, which constitute the four whorls of floral organs. Following initial description of the ABC model, it was demonstrated to facilitate flower development in a wide range of plant species, albeit with a number of species-specific modi-

show abstract

Molecular control of microsporogenesis in Arabidopsis

Cited by 89 publications

References 61 publications

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

Spatiotemporal Production of Reactive Oxygen Species by NADPH Oxidase Is Critical for Tapetal Programmed Cell Death and Pollen Development in Arabidopsis

Evidence of Human-to-Swine Transmission of the Pandemic (H1N1) 2009 Influenza Virus in South Korea

Contact Info

Product

Resources

About