Molecular Confirmation of Massive Taenia pisiformis Cysticercosis in One Rabbit in Poland

Abstract

The aim of this study was to provide molecular characterization, together with phylogenetic analysis, of Taenia pisiformis cysts isolated from rabbit. On the basis of morphological features and molecular analysis, the cysticerci were identified as T.pisiformis metacestodes. PCR was performed with three different protocols to obtain partial sequences of 12S ribosomal RNA (12S rRNA), NADH dehydrogenase subunit 1 (nad1), and cytochrome oxidase subunit 1 (cox1) of Taenia spp. The products from the PCRs were sequenced. Interpretation of the sequencing results of the obtained amplicons, by comparing them with the GenBank database, proved that the causative agent, in this case, was T. pisiformis. The phylogenetic analysis of the received sequences identified a new haplotype. The received data can be used to supplement the species description. To our knowledge, this is the first molecular confirmation of T. pisiformis metacestodes infection in the rabbit, in Poland.

Molecular Confirmation of Taenia crassiceps Cysticercosis in a Captive Ring-Tailed Lemur (Lemur catta) in Poland

“…The second PCR employed in the present study amplified a partial sequence of cytochrome c oxidase subunit 1 ( cox1 fragment gene) using a protocol designed by Bowles et al [ 44 ] and modified by Casulli et al [ 45 ], which has been described previously [ 43 ] and is reported in Table 1 . The PCR amplified a fragment to about 446 bp using primers CO1F and CO1R ( Table 1 ).…”