Molecular confirmation of <i>Rickettsia parkeri</i> in humans from Southern Mexico

Torres–Castro, Marco; Sánchez‐Montes, Sokani; Colunga‐Salas, Pablo; Noh-Pech, Henry; Reyes‐Novelo, Enrique; Rodríguez-Vivas, Róger Iván

doi:10.1111/zph.12927

Cited by 5 publications

(5 citation statements)

References 18 publications

(50 reference statements)

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“…This study presents the unusual presence of R. parkeri in synanthropic rodents ( M. musculus ) and domiciled dogs from a rural community in southeastern Mexico. These findings involve, for the first time M. musculus as accidental hosts in the epidemiological cycle of this Rickettsia species endemic to the study region (Peniche‐Lara & Lara‐Perera, 2022; Torres‐Castro, Sánchez‐Montes, et al, 2022), and the case of the dogs, they reinforce their participation in the said epidemiological cycle (Sánchez‐Montes et al, 2021) after the identification of this Rickettsia species in a domiciliated dog from the study region by Ojeda‐Chi et al (2019).…”

Section: Discussionmentioning

confidence: 79%

“…However, these ectoparasites have not been involved in the zoonotic transmission of R. parkeri . Recently, Peniche‐Lara and Lara‐Perera (2022) and Torres‐Castro, Sánchez‐Montes, et al (2022) reported the first cases of R. parkeri in symptomatic and asymptomatic inhabitants of Yucatán (southeastern Mexico). These records are relevant for public health because they show the circulation of this Rickettsia species among the inhabitants of Mexico (Sánchez‐Montes et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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An unusual identification ofRickettsia parkeriin synanthropic rodents and domiciliated dogs of a rural community from Yucatán, Mexico

Arroyo‐Ramírez,

Lugo‐Caballero,

Panti‐May

et al. 2023

Zoonoses and Public Health

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Rickettsia parkeri belongs to the spotted fever group (SFG) of the Rickettsia genus. This bacterium causes mild rickettsiosis in humans and is mainly transmitted by Amblyomma ticks. Its medical importance is emerging in the Americas, including Mexico. Synanthropic rodents and domiciled dogs participate as accidental hosts in epidemiological cycles of Rickettsia of the SFG. The aim is to report the presence of R. parkeri in synanthropic rodents and domiciled dogs from a rural community of Yucatán, Mexico. Rodents were captured, and plasma samples were taken from dogs in 48 households from Ucú, Yucatán, Mexico. A spleen sample (rodents) and plasma (dogs) were used in the propagation of Rickettsia on Vero cells. These infected cells were used in the extraction of genomic DNA. Rickettsia DNA was identified using a semi‐nested PCR (snPCR); some products were sent for sequencing. The recovered sequences were analysed with bioinformatics programs, and a phylogenetic tree was built to determine the Rickettsia species. One hundred animals were sampled: 36 synanthropic rodents and 64 dogs. The snPCR evidenced the presence of Rickettsia DNA in 10 rodents (10/36, 27.8%) and 18 dogs (18/64, 28.1%), which represents a global frequency of 28% (28/100) in this study. The bioinformatics analysis yielded homology to R. parkeri and was demonstrated in the phylogenetic tree. The first evidence of the presence of R. parkeri in synanthropic rodents (Mus musculus) from Mexico is presented; likewise, the participation of domestic dogs in the transmission cycle of this bacterium with potential importance in public health is confirmed.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

An unusual identification ofRickettsia parkeriin synanthropic rodents and domiciliated dogs of a rural community from Yucatán, Mexico

Arroyo‐Ramírez,

Lugo‐Caballero,

Panti‐May

et al. 2023

Zoonoses and Public Health

Self Cite

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“…In all PCRs, the DNA of Rickettsia conorii was used as a positive control. The negative control was the reaction mixture (primers plus reagents) without DNA [ 10 , 18 , 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, studies in a rural locality in the State of Yucatan, Mexico, performed by Torres-Castro et al [ 18 , 19 , 20 ], found that 90% of the people exposed to Rickettsia sp. infection (antibodies) were dog owners, and 70% had more than one dog in their household.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Study of the Occurrence of Typhus Group Rickettsia Natural Infection in Domiciliated Dogs from a Rural Community in South-Eastern Mexico

Torres–Castro

Reyes‐Novelo²,

Bolio-González³

et al. 2022

Animals

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The aim is to describe the Typhus group (TG) Rickettsia infection in dogs and to identify factors associated with this infection. We collected blood samples and gathered exposure and clinical data of 142 dogs from a rural community of Yucatan. The Rickettsia group was determined by semi-nested PCR. Generalized linear models with binomial error distribution were used to model the associated factors from the dog sample for risk ratio (RR) estimation. Thirty-four dogs (23.9%) showed molecular evidence of TG Rickettsia DNA. The multivariate model showed that mixed-breed dogs (RR = 0.06) and dogs that had received antiparasitic treatment (RR = 0.049) had a lower risk of getting infected, taking as reference the purebred group and the non-treated dogs, respectively. Looking at variable interactions, adult dogs without outdoor activities had a lower infection risk than puppies (RR = 0.26). Among dogs with antiparasitic treatment, females had a higher infection risk than male dogs (RR = 26.2). The results showed enzootic TG Rickettsia circulation in dogs of a rural community. The factors outdoor activities, age and previous antiparasitic treatment, as well as the clinical variables signs of hemorrhages and epistaxis, were associated with a less chance of natural infection in the studied dogs. Prevention and control of the enzootic transmission risk of TG Rickettsia should help to reduce the potential zoonotic transmission of this pathogen.

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“…The pathogenicity of R. parkeri for domestic and wild canids remains unknown, whereas this rickettsial species is well known as the second most frequently reported human rickettsial agent in the American continent responsible for eschar-associated spotted fever [ 99 ].…”

Section: Rickettsia Sppmentioning

confidence: 99%

Arthropod-Borne Pathogens in Wild Canids

Ebani

Nardoni

Mancianti

2023

Veterinary Sciences

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Wild canids, as well as other wild animal species, are largely exposed to bites by ticks and other hematophagous vectors where the features favoring their presence and spread are found in wooded and semi-wooded areas. Much of the information about arthropod-borne infections concerns domestic and companion animals, whereas data about these infections in wild canids are not exhaustive. The present study is a narrative review of the literature concerning vector-borne infections in wild canids, highlighting their role in the epidemiology of arthropod-borne bacteria and protozoa.

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Molecular confirmation of Rickettsia parkeri in humans from Southern Mexico

Cited by 5 publications

References 18 publications

An unusual identification ofRickettsia parkeriin synanthropic rodents and domiciliated dogs of a rural community from Yucatán, Mexico

An unusual identification ofRickettsia parkeriin synanthropic rodents and domiciliated dogs of a rural community from Yucatán, Mexico

Epidemiological Study of the Occurrence of Typhus Group Rickettsia Natural Infection in Domiciliated Dogs from a Rural Community in South-Eastern Mexico

Arthropod-Borne Pathogens in Wild Canids

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